Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,517,283 T→G 8.8% V158G (GTG→GGG) ‡ murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,517,2830TG 8.8% 216.6 / 9.7 77V158G (GTG→GGG) ‡murEUDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base T (33/37);  new base G (3/4);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

CGGCAAGACAAGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGACCACCTCGACTAT  >  NC_002947/1517143‑1517431
                                                                                                                                            |                                                                                                                                                    
cGGCAAGACAAGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCACGGCAATCgg                                                                                                                                              <  1:409270/149‑2 (MQ=255)
   cAAGACAAGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGAccc                                                                                                                                           <  3:240047/149‑1 (MQ=255)
    aaGACAAGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCACGGCAATCgggtcg                                                                                                                                          <  8:351613/149‑6 (MQ=255)
        cAAGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAAc                                                                                                                                      <  1:227770/149‑1 (MQ=255)
         aaGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAAc                                                                                                                                      >  1:150967/1‑148 (MQ=255)
           gtgtTACCCAACTGGTCGCCCGGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCACGGCAATCgggt                                                                                                                                            <  7:84234/140‑4 (MQ=255)
           gtgtTACCCAACTGGTCGCCCGGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGACTGACCACGCCCGACCCGATTGCACGGCAATccgga                                                                                                                                            >  8:84234/1‑136 (MQ=255)
           gtgaTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGAc                                                                                                                                   <  6:24106/149‑1 (MQ=255)
            tgtTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGAc                                                                                                                                   >  4:17508/1‑148 (MQ=255)
              gctttcAACTGGTCGCCCAGGGGCGCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGGCGTGGAATCGACCCTCAACGACCTc                                                                                                                                <  6:224630/144‑1 (MQ=255)
                aCCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGa                                                                                                                                              <  1:60495/133‑1 (MQ=255)
                aCCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGa                                                                                                                                              >  2:60495/1‑133 (MQ=255)
                   cAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGa                                                                                                                            >  7:64671/1‑148 (MQ=255)
                      cTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAgg                                                                                                                        <  6:48042/149‑1 (MQ=255)
                         gTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAggcgg                                                                                                                     >  8:263723/1‑149 (MQ=255)
                          tCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAggcggc                                                                                                                    >  7:206127/1‑149 (MQ=255)
                          tCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAggcggc                                                                                                                    >  7:337896/1‑149 (MQ=255)
                          tCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAggcggc                                                                                                                    >  8:99007/1‑149 (MQ=255)
                                    gcTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCg                                                                                                          >  2:18479/1‑149 (MQ=255)
                                    gcTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCg                                                                                                          >  8:115004/1‑149 (MQ=255)
                                    gcTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCg                                                                                                          >  8:45444/1‑149 (MQ=255)
                                     cTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCTGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGt                                                                                                         <  3:339017/149‑1 (MQ=255)
                                             gCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATg                                                                                                  >  5:346885/1‑148 (MQ=255)
                                              cTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCAttg                                                                                                 <  7:263723/148‑3 (MQ=255)
                                              cTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATgg                                                                                                 <  2:81567/148‑1 (MQ=255)
                                              cTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATg                                                                                                  <  8:64671/147‑1 (MQ=255)
                                                 ggCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGaa                                                                                               <  2:150967/147‑1 (MQ=255)
                                                             ggCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGc                                                                                 <  5:255105/149‑1 (MQ=255)
                                                              gCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGACCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACACTCAACGACCTCAAGAAAGGCGGCGCCATGGCCGTGGCCATGGAAGTGTCTTCCCATGc                                                                                 >  7:27945/1‑148 (MQ=255)
                                                               ccTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGccc                                                                               >  4:378484/1‑149 (MQ=255)
                                                                cTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCt                                                                              <  1:6587/149‑1 (MQ=255)
                                                                cTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAACGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCt                                                                              >  8:171781/1‑149 (MQ=255)
                                                                   aTTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCTTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTg                                                                             <  1:338575/147‑1 (MQ=255)
                                                                   aTTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTg                                                                             >  8:334266/1‑147 (MQ=255)
                                                                   aTTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTg                                                                             <  1:37676/147‑1 (MQ=255)
                                                                    ttGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAg                                                                          <  1:18479/149‑1 (MQ=255)
                                                                       gctcgcTGGGTACGGGTTTCTAGGGCGAGTTGCAGAGCGGCCGGCTGACGACGACCGACCCGTTTGCCGTGCAATCGACCCTCTACGACCTCATGAAAGGCGGCGCCAGGGCCGTGGCCATGTAAGTGTCTTCCCATGCGCTGGAGCaa                                                                       <  8:27945/144‑1 (MQ=255)
                                                                       gCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGc                                                                         >  2:98891/1‑147 (MQ=255)
                                                                        cACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAg                                                                      <  6:346885/149‑1 (MQ=255)
                                                                         aCCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAgg                                                                     <  7:334266/149‑1 (MQ=255)
                                                                               ggTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCgg                                                                 <  7:99007/147‑1 (MQ=255)
                                                                                gTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGTCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGtt                                                              >  1:84230/1‑149 (MQ=255)
                                                                                  aCCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCtt                                                                                        >  3:286622/1‑121 (MQ=255)
                                                                                  aCCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCtt                                                                                        <  4:286622/121‑1 (MQ=255)
                                                                                      gTTTCTACGGCGAGTTGAAGATCGGCCTCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGACATTGAAGTGTCTTCCCATGCCCTGTAGCAAGGCAGGGTTGCCGc                                                         >  8:194453/1‑148 (MQ=255)
                                                                                               gCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTc                                               <  1:98891/149‑1 (MQ=255)
                                                                                               gCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTc                                               <  8:337896/149‑1 (MQ=255)
                                                                                                      gCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCACTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATcg                                        >  6:211498/1‑149 (MQ=255)
                                                                                                       cAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAAc                                                                                                                                      <  5:243928/54‑1 (MQ=255)
                                                                                                       cAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAAc                                                                                                                                      >  6:243928/1‑54 (MQ=255)
                                                                                                        agagCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGCCATcgcg                                      >  3:247438/1‑149 (MQ=255)
                                                                                                         gagCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCgg                                     >  2:406064/1‑149 (MQ=255)
                                                                                                               ccgccTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCgg                                     <  2:365256/143‑1 (MQ=255)
                                                                                                               ccgccTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCgg                                     >  1:365256/1‑143 (MQ=255)
                                                                                                               ccgccTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGtgatga                               <  7:171781/149‑1 (MQ=255)
                                                                                                               ccgccTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGtgatga                               <  3:17508/149‑1 (MQ=255)
                                                                                                                  ccTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGAGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTCGAATTCGACATCGCGGTGATGAcc                             >  4:254865/1‑148 (MQ=255)
                                                                                                                   cTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCaa                           <  1:406064/149‑1 (MQ=255)
                                                                                                                      aCCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAAc                          <  7:115004/147‑1 (MQ=255)
                                                                                                                        cACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGt                      >  6:175995/1‑149 (MQ=255)
                                                                                                                        cACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGt                      >  6:169822/1‑149 (MQ=255)
                                                                                                                         aCGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTc                                               >  7:378656/1‑123 (MQ=255)
                                                                                                                         aCGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTc                                               <  8:378656/123‑1 (MQ=255)
                                                                                                                             ccgacccgaTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGtgatg                                <  8:254341/134‑1 (MQ=255)
                                                                                                                             ccgacccgaTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGtgatg                                >  7:254341/1‑134 (MQ=255)
                                                                                                                                acccgaTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGac              <  6:145253/149‑1 (MQ=255)
                                                                                                                                 cccgaTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGacc             <  7:45444/149‑1 (MQ=255)
                                                                                                                                 cccgaTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATCCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGacc             >  8:369034/1‑149 (MQ=255)
                                                                                                                                  tcgaTTGCACGGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACt                                                      <  4:392043/106‑1 (MQ=255)
                                                                                                                                  tcgaTTGCACGGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACt                                                      >  3:392043/2‑107 (MQ=255)
                                                                                                                                  tcgaTTGCACGGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGacca            >  2:103902/2‑149 (MQ=255)
                                                                                                                                  ccgaTTGCCCTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCCCCAGGGCCGTGGCCATAGAAATGTCTTCCCATGCCCTCCAGCAAGGCCCGGTTGCCGCACTGCAATTCGACATCGCGGTGATGACCAACCAGTCCCGccacca            >  8:358058/1‑149 (MQ=255)
                                                                                                                                   cgaTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGaccac           <  4:247438/149‑1 (MQ=255)
                                                                                                                                     aTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGACCACCt         <  5:175995/149‑1 (MQ=255)
                                                                                                                                     aTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGACCACCt         <  5:169822/149‑1 (MQ=255)
                                                                                                                                        gCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGACCACCTCg       <  3:378484/148‑1 (MQ=255)
                                                                                                                                         ccGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGACCCCCTCGAc     >  4:6665/1‑149 (MQ=255)
                                                                                                                                            tGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGACCACCTCGACTAt  <  2:84230/149‑1 (MQ=255)
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CGGCAAGACAAGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGACCACCTCGACTAT  >  NC_002947/1517143‑1517431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑