| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,521,248 | C→A | 8.7% | Q169K (CAG→AAG) | murD → | UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,521,248 | 0 | C | A | 8.7% | 202.0 / 8.9 | 69 | Q169K (CAG→AAG) | murD | UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase |
| Reads supporting (aligned to +/- strand): ref base C (29/33); new base A (3/3); total (32/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01 | |||||||||||
GCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGGTCAACC > NC_002947/1521104‑1521395 | gCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTtccagcttccagct < 2:77271/149‑1 (MQ=255) cAAGAGCTCCGGCTCGACGCTGGTCGGTGAAATGGCCGCGATGGCCGGCAATCGCGTGGCGGTGGGCGGTAACCTGGGTTCCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGGTTCCAGCTgg < 2:384103/149‑1 (MQ=255) cAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTgg < 2:2499/149‑1 (MQ=255) cAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTg > 8:150198/1‑148 (MQ=255) cAAGAGCACCGTCACGACGCTGGTCGGTGAAAAGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCATCCAGCTg > 1:384103/1‑148 (MQ=255) agCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAAc < 4:102840/149‑1 (MQ=255) cGTCACGACTCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTGGAAGCTGGaaagctcca < 2:51899/149‑7 (MQ=255) cGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACt > 2:130107/1‑148 (MQ=255) cGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACt > 2:33562/1‑148 (MQ=255) tCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGAc < 7:117161/149‑1 (MQ=255) gacgCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGc < 5:259246/149‑1 (MQ=255) aatgcccaTGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTATCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCg < 1:176016/142‑1 (MQ=255) gTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGa‑‑a < 7:150198/149‑1 (MQ=255) aaaTGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTCCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTg < 2:361291/149‑1 (MQ=255) aaTGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTgg > 1:212730/1‑149 (MQ=255) gccAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGt > 4:34766/1‑149 (MQ=255) cAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTc > 3:1281/1‑149 (MQ=255) aaGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCt < 4:1281/149‑1 (MQ=255) gCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGaa < 8:287067/149‑1 (MQ=255) gCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACt > 8:305798/1‑110 (MQ=255) gCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACt < 7:305798/110‑1 (MQ=255) aaCGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGc < 5:107100/149‑1 (MQ=255) gcgTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCg > 5:102585/1‑147 (MQ=255) gTGGCCGTGGGCGGTAACCTCGGAACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGa < 7:325137/149‑1 (MQ=255) tGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGAc < 7:7308/149‑1 (MQ=255) gCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACca < 3:34766/149‑1 (MQ=255) cGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACcaca > 7:180557/1‑149 (MQ=255) tGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATg > 5:388964/1‑149 (MQ=255) ggCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATgg < 8:152692/148‑1 (MQ=255) gCGTTAACCTCGGGACCCCTGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGAc < 4:236752/149‑1 (MQ=255) gCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGTTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCTTTCTGAACATCAGCGAAGACCACATGGAc < 8:364519/149‑1 (MQ=255) tAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGc > 1:188849/1‑148 (MQ=255) aCCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCtat < 7:260116/149‑1 (MQ=255) cTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAg > 3:4346/1‑149 (MQ=255) ccccGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCtt < 5:394404/149‑1 (MQ=255) cccGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTc > 2:51388/1‑149 (MQ=255) cccGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTc > 7:137584/1‑149 (MQ=255) aCTAGACCTGATGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑T‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCt < 4:118891/149‑1 (MQ=255) cTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCt > 4:45319/1‑101 (MQ=255) cTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCt < 3:45319/101‑1 (MQ=255) cTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTa < 6:102585/149‑1 (MQ=255) gACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTacca > 1:257791/1‑149 (MQ=255) gACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTac < 1:33562/147‑1 (MQ=255) aCCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTaccac < 7:135664/149‑1 (MQ=255) aCCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTaccac < 1:153153/149‑1 (MQ=255) ccGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCa > 4:240860/1‑96 (MQ=255) ccGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCa < 3:240860/96‑1 (MQ=255) cGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGc > 1:272369/1‑124 (MQ=255) cGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGc < 2:272369/124‑1 (MQ=255) cGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCaaa > 3:251470/1‑148 (MQ=255) gATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAaca > 2:276694/1‑149 (MQ=255) cATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCgg < 2:188849/148‑1 (MQ=255) aTCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTg < 5:179007/135‑1 (MQ=255) aTCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTg > 6:179007/1‑135 (MQ=255) ttcGTGCTGGTGCTTTTGAGCTTCGAGCTGGTTAAAACTGATCAGCTCAACTCCGATTAGGTGCCCACCGTACTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCcg < 1:130107/147‑1 (MQ=255) tACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCgg > 1:270666/1‑149 (MQ=255) tACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCgg > 8:206438/1‑149 (MQ=255) tGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGcc > 2:282882/1‑149 (MQ=255) cagtggtttCCAGCTGGAAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCgg > 5:103605/7‑142 (MQ=255) cagtggtttCCAGCTGGAAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCgg < 6:103605/136‑1 (MQ=255) ggAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGc > 5:129194/1‑148 (MQ=255) gAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGc < 8:180557/147‑1 (MQ=255) gAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGc > 4:273171/1‑147 (MQ=255) gAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCa < 2:270666/149‑1 (MQ=255) aGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGTGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGc > 1:9075/1‑98 (MQ=255) aGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGTGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGc < 2:9075/98‑1 (MQ=255) gCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAgg > 8:99114/1‑149 (MQ=255) gtttCCAGCTGGAAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCTGTGCCCGCAAggg > 6:283401/2‑148 (MQ=255) tttCCAGCTGGAAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAac < 4:204793/120‑1 (MQ=255) tttCCAGCTGGAAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAac > 3:204793/1‑120 (MQ=255) ttccagctggaagcTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtgg > 6:136243/1‑149 (MQ=255) tccagcttccagctGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtggt > 6:284877/1‑149 (MQ=255) tccagctggaagctGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtgg < 5:136243/148‑1 (MQ=255) cagcttccagctGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtggtgg < 7:99114/149‑1 (MQ=255) cagcttccagctGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtggtg < 4:4346/148‑1 (MQ=255) agcttccagctGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtggtggt < 7:206438/149‑1 (MQ=255) cttccagctGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGGTc > 6:54197/1‑148 (MQ=255) ttccagctGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGAttt > 3:338799/1‑121 (MQ=255) ttccagctGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGAttt < 4:338799/121‑1 (MQ=255) ccagctGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGGTCAAcc > 4:125856/1‑149 (MQ=255) cagctGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGGTCAAcc < 2:257791/148‑1 (MQ=255) | GCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGA‑‑A‑GTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGGTCAACC > NC_002947/1521104‑1521395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |