Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,625,207 G→T 7.1% intergenic (+30/+27) PP_1425 → / ← nadB hypothetical protein/L‑aspartate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,625,2070GT 7.1% 178.7 / 4.4 68intergenic (+30/+27)PP_1425/nadBhypothetical protein/L‑aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base G (37/26);  new base T (3/2);  total (40/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.52e-01

GGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACT  >  NC_002947/1625066‑1625354
                                                                                                                                             |                                                                                                                                                   
gggggAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCcgc                                                                                                                                              >  8:195870/1‑149 (MQ=255)
    gAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAg                                                                                                                                          >  6:352341/1‑149 (MQ=255)
      aaGCCAGTGTATACCTCAGGAGGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGGTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCCGGCTCGCCCGCGAAgaa                                                                                                                                        <  3:279027/149‑3 (MQ=255)
       aGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGTGCATAGATCGAGACCTGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGcc                                                                                                                                       >  2:383818/1‑149 (MQ=255)
       aGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGc                                                                                                                                        >  8:365214/1‑148 (MQ=255)
        gCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCa                                                                                                                                      <  7:365214/149‑1 (MQ=255)
        gCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCa                                                                                                                                      >  3:175753/1‑149 (MQ=255)
            gtgtATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCAGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCacgac                                                                                                                                  >  8:14088/1‑149 (MQ=255)
                ataCCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGa                                                                                                                                                    <  1:385765/127‑1 (MQ=255)
                ataCCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGa                                                                                                                                                    >  2:385765/1‑127 (MQ=255)
                ataCCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGcc                                                                                                                               >  5:241661/1‑148 (MQ=255)
                   ccTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGata                                                                                                                           >  6:92215/1‑149 (MQ=255)
                   ccTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGat                                                                                                                            <  5:401406/148‑1 (MQ=255)
                    ctaaGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCTGTTGAAGTTCAGCCGGCTGCGCTGGGCTGCGGAGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGaa                                                                                                                                           >  7:257835/4‑132 (MQ=255)
                    cTCAGGATGCGGTAGGGGCTGACGAGCATCGGCTCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGaa                                                                                                                                           <  8:257835/132‑1 (MQ=255)
                    cTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCGGCGAAGGCCCCGCCGCCGattg                                                                                                                          >  2:364758/1‑147 (MQ=255)
                    cTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGatat                                                                                                                          >  6:372927/1‑149 (MQ=255)
                    cTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGatat                                                                                                                          >  7:308940/1‑149 (MQ=255)
                    cTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGata                                                                                                                           >  8:235772/1‑148 (MQ=255)
                           tGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCg                                                                                                                   >  5:4258/1‑149 (MQ=255)
                           tGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCg                                                                                                                   >  1:32350/1‑149 (MQ=255)
                               cTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGtt                                                                                                               <  5:92215/149‑1 (MQ=255)
                                      cTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATg                                                                                                        >  7:26438/1‑149 (MQ=255)
                                      cTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCAGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATg                                                                                                        <  7:14088/149‑1 (MQ=255)
                                          ctaGCTTCGGGGCTTGTGGGTGCGTTTGAAGTTCAGGCGGCGGCGCTGGGCTGCGGTGTGCCGGGAATATATCTAGAGGGGGTTTGTTTCTTCGGGGGCGAGCCGGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGgtg                                                                                                     <  2:32350/146‑1 (MQ=255)
                                           gAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCTCGCCCGCGAAga                                                                                                                                         >  3:366956/1‑110 (MQ=255)
                                           gAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCTCGCCCGCGAAga                                                                                                                                         <  4:366956/111‑2 (MQ=255)
                                               aTCGGCGCTTGCGGGTGCGGTTTAAGTTCAGCCGGCGGCGCTGGGCTGGGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTg                                                                                               <  8:55196/149‑1 (MQ=255)
                                                gcGGCGCTTGCGGGTGGGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGCCATAGATCGAGCCCGGGTTATCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTgg                                                                                              <  2:383339/148‑1 (MQ=255)
                                                             gTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCAt                                                                                 >  2:9231/1‑149 (MQ=255)
                                                                   ttGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTgg                                                                           <  5:193631/149‑1 (MQ=255)
                                                                      aaGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGAt                                                                         >  7:5410/1‑148 (MQ=255)
                                                                         ttCCGCCGGGGGCGCTGGGGTGCGGTGAGCGGGGCATAGATCGATACGGGTTTAGGTTGTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATc                                                                     <  4:175753/149‑1 (MQ=255)
                                                                         ttCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATc                                                                     <  5:352341/149‑1 (MQ=255)
                                                                            aGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCa                                                                   <  1:9231/148‑1 (MQ=255)
                                                                            aGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAg                                                                  <  7:235772/149‑1 (MQ=255)
                                                                             gcgggcggcGGTGGGCTGCGGTGAGCCGGGAATAGATCGAGACGGGTTTAGCTTCTGCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAgt                                                                 <  8:26438/146‑1 (MQ=255)
                                                                                  cggggCTGGGGTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTa                                                            <  1:364758/149‑1 (MQ=255)
                                                                                   ggcgCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTaa                                                           >  2:247000/1‑149 (MQ=255)
                                                                                            cTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGa                                                                          >  3:1531/1‑125 (MQ=255)
                                                                                                   gAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGTCCACGACGCCGATATCAGAGCGGGGTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCttt                                           >  6:8422/1‑149 (MQ=255)
                                                                                                   gAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCttt                                           >  6:14272/1‑149 (MQ=255)
                                                                                                    aGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTc                                          <  6:4258/149‑1 (MQ=255)
                                                                                                       cGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCtt                                       <  7:195870/149‑1 (MQ=255)
                                                                                                        ctatctAGAGCGAGACCGTGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCtt                                       <  1:255913/143‑1 (MQ=255)
                                                                                                           cATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTgcgc                                   >  7:205257/1‑149 (MQ=255)
                                                                                                            aTAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCt                                  >  1:340220/1‑149 (MQ=255)
                                                                                                             tAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTg                                 <  1:247000/149‑1 (MQ=255)
                                                                                                              aGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTg                                 >  6:105862/1‑148 (MQ=255)
                                                                                                               gATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCtt                                       >  2:255913/1‑141 (MQ=255)
                                                                                                                 tCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATg                             >  7:94178/1‑149 (MQ=255)
                                                                                                                   gagaCCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTgc                                     <  6:161269/139‑1 (MQ=255)
                                                                                                                   gagaCCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTgc                                     >  5:161269/1‑139 (MQ=255)
                                                                                                                   gagaCCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATgg                            <  8:94178/148‑1 (MQ=255)
                                                                                                                   gagaCCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGc                           <  8:308940/149‑1 (MQ=255)
                                                                                                                    agaCCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCTAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCt                          >  6:110135/1‑149 (MQ=255)
                                                                                                                      aCCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGa                        >  2:288220/1‑149 (MQ=255)
                                                                                                                        cGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACa                      <  5:105862/149‑1 (MQ=255)
                                                                                                                               tggccttcGCGGGCTCGCCCGCGAAGACCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGatcat               >  1:230872/5‑149 (MQ=255)
                                                                                                                               gCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGatcat               <  8:5410/149‑1 (MQ=255)
                                                                                                                                cttcttCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGatcat               >  4:335282/1‑148 (MQ=255)
                                                                                                                                 ttcttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGatcat               >  5:317307/1‑147 (MQ=255)
                                                                                                                                  tcttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAg            >  3:183773/1‑149 (MQ=255)
                                                                                                                                   cttcGCGGGCTCGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTCCGCTGCATGGCTGACAGGATCATAAGc           >  8:224834/1‑149 (MQ=255)
                                                                                                                                   cttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGc           >  1:51962/1‑149 (MQ=255)
                                                                                                                                       gcgGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCTAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCg        <  5:110135/148‑1 (MQ=255)
                                                                                                                                           gCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCAc   >  1:273478/1‑149 (MQ=255)
                                                                                                                                            cGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACt  >  2:284776/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
GGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACT  >  NC_002947/1625066‑1625354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑