Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 186,766 T→C 6.2% T51A (ACC→GCC)  PP_0163 ← transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947186,7660TC 6.2% 183.9 / 2.0 66T51A (ACC→GCC) PP_0163transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (34/26);  new base C (2/2);  total (37/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

ACGCGAAGGGCATAGGTGTCCACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGCACATTGCTCAATTTGATTTTCTTGGC  >  NC_002947/186621‑186913
                                                                                                                                                 |                                                                                                                                                   
aCGCGAAGGGCATAGGTGTCCACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGc                                                                                                                                                  <  3:141954/149‑1 (MQ=255)
  gcgAAGGGCATAGGTGTCCACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGa                                                                                                                                                <  2:45082/149‑1 (MQ=255)
        ggCATAGGTGTCCACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCtt                                                                                                                                          <  5:165989/149‑1 (MQ=255)
          cATAGGTGTCCACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCttgt                                                                                                                                        <  1:192849/149‑1 (MQ=255)
          cATAGGTGTCCACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCttgt                                                                                                                                        >  5:96401/1‑149 (MQ=255)
          cATAGGTGTCCACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGAAGCCGTACAGGGAGGCGAGCTCttgt                                                                                                                                        >  4:87308/1‑149 (MQ=255)
             aGGTGTCCACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTgg                                                                                                                                     >  6:111958/1‑149 (MQ=255)
             aGGTGTCCACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTgg                                                                                                                                     >  2:357195/1‑149 (MQ=255)
               gtgtCCACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTgg                                                                                                                                     >  8:34789/1‑147 (MQ=255)
               gtgtCCACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTgg                                                                                                                                     <  7:34789/147‑1 (MQ=255)
               gtgtCCACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGCGGCGAGCTCTTGTTGGc                                                                                                                                    >  5:15428/1‑148 (MQ=255)
               gtgtCCACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGCGGCGAGCTCTTGTTGGc                                                                                                                                    >  1:360642/1‑148 (MQ=255)
               gtgtCAACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGCGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCGCTTGTTGGc                                                                                                                                    >  7:141868/1‑148 (MQ=255)
                        gCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCttgtt                                                                                                                                       <  6:47894/136‑1 (MQ=255)
                        gCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGAGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCttgtt                                                                                                                                       >  5:47894/1‑136 (MQ=255)
                           tCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTAc                                                                                                                        >  8:253669/1‑148 (MQ=255)
                            ccTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTAc                                                                                                                        >  7:79356/1‑147 (MQ=255)
                               cGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAggg                                                                                                                   >  3:2120/1‑149 (MQ=255)
                                ggcgttCAGCGGTGCAACGACGGCGCGCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGGATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAgggg                                                                                                                  <  6:10848/144‑1 (MQ=255)
                                gCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAgggg                                                                                                                  <  5:111958/149‑1 (MQ=255)
                                gCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAggg                                                                                                                   <  6:115932/148‑1 (MQ=255)
                                 ccGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAggggg                                                                                                                 <  8:79356/149‑1 (MQ=255)
                                    aTCAGCGGTGCAACGACGGCGCCCTTGTACATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCaa                                                                                                              <  2:164316/149‑1 (MQ=255)
                                           gTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCa                                                                                                       >  2:133508/1‑149 (MQ=255)
                                           gTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCa                                                                                                       >  7:360002/1‑149 (MQ=255)
                                              cAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGCGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGGTGGCGAACGGCAGTACCAGGCGGCAAGCGACCATc                                                                                                     >  6:287207/1‑148 (MQ=255)
                                               aaCGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCg                                                                                                    <  4:170984/148‑1 (MQ=255)
                                                  gacgGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAAc                                                                                                                                <  7:340079/117‑1 (MQ=255)
                                                  gacgGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAAc                                                                                                                                >  8:340079/1‑117 (MQ=255)
                                                       cgcCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGCCTGACGCCGTACAGGGTGGCGAGCTCTGGTTGGCGAACGGCCGTCCCAGGGGGCAAGCGACCAGCGAGCATGgcg                                                                                           >  8:83912/1‑149 (MQ=255)
                                                       cgcCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGgcg                                                                                           >  8:20221/1‑149 (MQ=255)
                                                              gtgCATCTCCAATTTCAACAGGGATTGAGCTTCGAGCTGGCGCAGGCCTTCGCGCACGGGCATGCAACTGACGCCGTACAGGATTGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGTGACCATCTAGAATGGCGATgag                                                                                      >  5:220624/1‑145 (MQ=255)
                                                                   tctcCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGt                                                                               >  1:280227/1‑149 (MQ=255)
                                                                     tcCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGttc                                                                             >  2:278533/1‑149 (MQ=255)
                                                                      ccACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGttct                                                                            >  4:331776/1‑149 (MQ=255)
                                                                       cACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGtt                                                                              <  8:360002/146‑1 (MQ=255)
                                                                                cAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCg                                                                         >  2:302143/1‑142 (MQ=255)
                                                                                cAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCg                                                                         <  1:302143/142‑1 (MQ=255)
                                                                                     aTTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATcacggcacgg                                                             >  1:364469/1‑149 (MQ=255)
                                                                                         cgcTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCt                                                         <  1:133508/149‑1 (MQ=255)
                                                                                            ttCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGAt                                                      >  4:64681/1‑149 (MQ=255)
                                                                                               gAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATgcg                                                   >  3:400472/1‑149 (MQ=255)
                                                                                                aGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGCGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCTCGGGCCTGATgcgg                                                  <  2:360642/149‑1 (MQ=255)
                                                                                                aGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGCGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATgcgg                                                  <  6:15428/149‑1 (MQ=255)
                                                                                                     gcgcAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCggcggc                                              <  2:364469/148‑1 (MQ=255)
                                                                                                     gcgcAGGGCTTCGCGCACGGGCATGCGACTGACGCCATACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCggcggc                                              >  1:271613/1‑148 (MQ=255)
                                                                                                            gCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTc                                      <  1:278533/149‑1 (MQ=255)
                                                                                                             cTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCg                                     >  7:97693/1‑149 (MQ=255)
                                                                                                                  cgcACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGc                                <  1:357195/149‑1 (MQ=255)
                                                                                                                  cgcACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGc                                <  4:400472/149‑1 (MQ=255)
                                                                                                                   gcACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGcc                               <  7:253669/149‑1 (MQ=255)
                                                                                                                       gggCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGca                           >  8:241739/1‑149 (MQ=255)
                                                                                                                         gCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTg                                  <  8:237657/140‑1 (MQ=255)
                                                                                                                         gCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTg                                  >  7:237657/1‑140 (MQ=255)
                                                                                                                         gCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGca                           <  3:331776/147‑1 (MQ=255)
                                                                                                                          cATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGCACAt                        <  7:20221/149‑1 (MQ=255)
                                                                                                                             gCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGCACATTGc                     >  5:166729/1‑149 (MQ=255)
                                                                                                                                  tGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGCACATTGCTCAAt                >  7:301745/1‑149 (MQ=255)
                                                                                                                                  tGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGCACATTGCTCAAt                >  6:279911/1‑149 (MQ=255)
                                                                                                                                    ccGCCGTACCGGGGGGCGAGCTCTTGTGGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGCGCATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGCACATTGCTCAAttt              <  7:83912/148‑1 (MQ=255)
                                                                                                                                      gCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGCACATTGCTCaa                 >  8:329433/1‑144 (MQ=255)
                                                                                                                                      gCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGCACATTGCTCaa                 <  7:329433/144‑1 (MQ=255)
                                                                                                                                      gCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGCACATTGCTCAATTTGa            >  4:171295/1‑149 (MQ=255)
                                                                                                                                      gCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGCACATTGCTCAATTTGa            <  5:279911/149‑1 (MQ=255)
                                                                                                                                              gggTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGCACATTGCTCAATTTGATTTTCTTg    >  1:405618/1‑149 (MQ=255)
                                                                                                                                              gggTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGCACATTGCTCAATTTGATTTTCTTg    >  7:30837/1‑149 (MQ=255)
                                                                                                                                                 tGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGCACATTGCTCAATTTGATTTTCTTGGc  >  4:110272/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ACGCGAAGGGCATAGGTGTCCACTGCATCCTCGCCGATCAGCGGTGCAACGACGGCGCCCTTGTGCATCTCCACTTTCAACAGGGATTGCGCTTCGAGCTGGCGCAGGGCTTCGCGCACGGGCATGCGACTGACGCCGTACAGGGTGGCGAGCTCTTGTTGGCGAACGGCAGTACCAGGGGGCAAGCGACCATCGAGAATGGCGCTGCGCAGGCGTTCTTCGATCACGGCACGGGCCTGATGCGGCGGCAACTGCTCGCTGGCCAGCACATTGCTCAATTTGATTTTCTTGGC  >  NC_002947/186621‑186913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑