Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,639,586 A→G 9.4% intergenic (‑176/‑135) czcR‑III ← / → PP_1439 response regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,639,5860AG 9.4% 144.8 / 5.3 53intergenic (‑176/‑135)czcR‑III/PP_1439response regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (18/30);  new base G (2/3);  total (20/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

GATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCA  >  NC_002947/1639438‑1639733
                                                                                                                                                    |                                                                                                                                                    
gATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGaa                                                                                                                                                      >  4:97702/1‑149 (MQ=255)
 aTTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAAc                                                                                                                                                     <  1:256991/149‑1 (MQ=255)
 aTTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAAc                                                                                                                                                     <  4:391469/149‑1 (MQ=255)
  ttCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTTGGAACg                                                                                                                                                    <  6:169694/149‑1 (MQ=255)
       gACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACggg                                                                                                                                                  <  8:100864/146‑1 (MQ=255)
       gACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACggg                                                                                                                                                  <  4:319376/146‑1 (MQ=255)
       gACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACggg                                                                                                                                                  <  3:177515/146‑1 (MQ=255)
        aCCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAc                                                                                                                                             <  7:45845/149‑1 (MQ=255)
              tAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCcgc                                                                                                                                         <  6:91703/148‑1 (MQ=255)
                   aCATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAagag                                                                                                                                   <  8:192301/149‑1 (MQ=255)
                   aCATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAaga                                                                                                                                    <  6:57110/148‑1 (MQ=255)
                     aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGc                                                                                                                                 <  5:115306/149‑1 (MQ=255)
                     aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGc                                                                                                                                 <  7:341675/149‑1 (MQ=255)
                      tCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACggg                                                                                                                                                  >  7:180358/1‑131 (MQ=255)
                      tCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACggg                                                                                                                                                  <  8:180358/131‑1 (MQ=255)
                        aGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCGGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGccc                                                                                                                               <  6:230/148‑1 (MQ=255)
                          ggTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGGCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGaa                                                                                                                            <  4:117114/149‑1 (MQ=255)
                            tCAAGCGCCCCAATGTTCCTGCATGAAAACAGGCCTGCGGTGCAGATAACGCCTTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAAGAGGCCCGAACa                                                                                                                          <  5:229596/149‑1 (MQ=18)
                            tCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGcc                                                                                                                                >  7:109085/1‑143 (MQ=255)
                            tCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGcc                                                                                                                                <  8:109085/143‑1 (MQ=255)
                            tCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACa                                                                                                                          <  3:97702/149‑1 (MQ=255)
                                    cccAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTAct                                                                                                                                            >  4:202167/1‑122 (MQ=25)
                                    cccAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTAct                                                                                                                                            <  3:202167/123‑2 (MQ=25)
                                    cccAATGTCACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCcaca                                                                                                                   >  4:185659/1‑148 (MQ=255)
                                          gTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGc                                                                                                             >  6:137917/1‑148 (MQ=255)
                                                gCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAcc                                                                                                                                            <  6:111148/111‑1 (MQ=255)
                                                gCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAcc                                                                                                                                            >  5:111148/1‑111 (MQ=255)
                                                            gCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAAt                                                                                          >  4:134713/1‑149 (MQ=255)
                                                             ccTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTTTTCGGGCCTATTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCTAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATc                                                                                         <  4:126206/149‑1 (MQ=255)
                                                             ccTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATc                                                                                         >  6:124675/1‑149 (MQ=255)
                                                                            aCGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACaa                                                                                                                <  6:334179/111‑1 (MQ=255)
                                                                            aCGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACaa                                                                                                                >  5:334179/1‑111 (MQ=255)
                                                                             cGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGcc                                                                         >  2:240838/1‑149 (MQ=255)
                                                                                    gTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCTAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTagc                                                                   <  3:185659/148‑1 (MQ=255)
                                                                                                ttATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGAAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTa                                                       <  1:240838/148‑1 (MQ=255)
                                                                                                      ggCTGCCTGTCCGGGCCTCTTCGCGCGTAAACCCGCGCCTGTAGCAACGGGTTTACCCGCGAAGCGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGc                                               <  8:256346/149‑1 (MQ=255)
                                                                                                              gtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCt                                        >  2:371082/1‑148 (MQ=255)
                                                                                                                  gggCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATgg                                                >  2:242270/1‑136 (MQ=255)
                                                                                                                  gggCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATgg                                                <  1:242270/136‑1 (MQ=255)
                                                                                                                    gCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGt                                 >  5:388123/1‑149 (MQ=255)
                                                                                                                      ctctTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGtta                               >  3:351357/1‑149 (MQ=255)
                                                                                                                      ctctTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGtta                               >  2:398554/1‑149 (MQ=255)
                                                                                                                           cgcgAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGttatta                            <  5:137917/147‑1 (MQ=255)
                                                                                                                           cgcgAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGTTATTAc                           >  8:182752/1‑148 (MQ=255)
                                                                                                                            gcgAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGTTATTACGa                         <  3:134713/149‑1 (MQ=255)
                                                                                                                              gggTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGTTATTACGa                         >  4:323065/3‑147 (MQ=37)
                                                                                                                              gggTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGTTATTACGa                         <  3:323065/145‑1 (MQ=37)
                                                                                                                              gAGTAAACCCGCGCCTGTAGGAACGGGTTTGCCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCGTCGCTAGG‑ATGGCCGTGTCTGATCGGTTATTACTAg                        >  1:143770/1‑148 (MQ=255)
                                                                                                                                    aCCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGTTATTACGAGGCAAaca                 <  5:124675/149‑1 (MQ=255)
                                                                                                                                      ccGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAACGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCCTTAGCCAGCCCTCGCTACG‑ATGGCCGGGTCGGATCGGTTATTACGAGGCATacac                >  8:372007/1‑148 (MQ=255)
                                                                                                                                       cgcgCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACTTTGCAGGAGTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGa              <  7:372007/149‑1 (MQ=255)
                                                                                                                                        gcgcCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGAt             >  7:260253/1‑149 (MQ=255)
                                                                                                                                        gcgcCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGAt             <  1:371082/149‑1 (MQ=255)
                                                                                                                                                 ggAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGa     <  4:351357/148‑1 (MQ=255)
                                                                                                                                                    aCGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGAAAAAAAGTGAGTGATCGGTTATTACGAGGCAAACCCGATGAACCTGccc   >  1:136090/1‑148 (MQ=255)
                                                                                                                                                    aCGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCa  >  7:41403/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGG‑ATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCA  >  NC_002947/1639438‑1639733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑