Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,677,428 G→C 6.3% A411P (GCG→CCG) ‡ thrC → threonine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,677,4280GC 6.3% 291.7 / 6.2 90A411P (GCG→CCG) ‡thrCthreonine synthase
Reads supporting (aligned to +/- strand):  ref base G (43/41);  new base C (4/2);  total (47/43)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

CGTCAGCGACGAGCAAACCTGCGAGACCATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCA  >  NC_002947/1677283‑1677575
                                                                                                                                                 |                                                                                                                                                   
cGTCAGCGACGAGCAAACCTGCGAGACCATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACcgcg                                                                                                                                                   >  3:347131/1‑148 (MQ=255)
    aGCGACGAGCAAACCTGCGAGACCATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCAccc                                                                                                                                              <  4:347131/149‑1 (MQ=255)
          gaGCAAACCTGCGAGACCATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCAGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTc                                                                                                                                          >  3:158553/1‑147 (MQ=255)
              aaaCCTGCGAGACCATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTc                                                                                                                                    <  3:28456/149‑1 (MQ=255)
                aCCTGCGAGACCATCGCAGAAGTGTTCGCCGCCACCTGTGAAGTGCTCGACCCGCATACCGGAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTccc                                                                                                                                  <  2:242532/149‑1 (MQ=255)
                aCCTGCGAGACCATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTccc                                                                                                                                  >  4:99929/1‑149 (MQ=255)
                aCCTGCGAGACCATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTccc                                                                                                                                  <  8:193467/149‑1 (MQ=255)
                   tGCGAGACCATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCGCGGTACCCAGCAccac                                                                                                                                             <  1:200239/135‑3 (MQ=255)
                   tGCGAGACCATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCGCGGTACCCAGCAccac                                                                                                                                             >  2:200239/1‑133 (MQ=255)
                    gCGAGACCATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCgg                                                                                                                                >  2:317645/1‑147 (MQ=255)
                     cGAGACCATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGaa                                                                                                                              <  3:155004/148‑1 (MQ=255)
                        gaCCATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCgg                                                                                                                          >  5:148645/1‑149 (MQ=255)
                          ccATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTg                                                                                                                        <  1:203680/149‑1 (MQ=255)
                           cATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTgg                                                                                                                       <  1:181076/149‑1 (MQ=255)
                           cATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTgg                                                                                                                       <  3:288467/149‑1 (MQ=255)
                             tCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGgag                                                                                                                     <  1:221387/149‑1 (MQ=255)
                               gCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGaa                                                                                                                   <  5:142588/149‑1 (MQ=255)
                                   aaGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGcc                                                                                                               >  2:236468/1‑149 (MQ=255)
                                   aaGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCAGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCCGTCAAGTTCCCGGAAGCGGTGGAGAAGGcc                                                                                                               >  5:128285/1‑149 (MQ=255)
                                      tgtTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGgt                                                                                                            <  3:99929/149‑1 (MQ=255)
                                        ttagccgccACCGGTGAAGTGCTCGACCCCCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTCCCGCCGCAGTCTGGACACGCCAAAGGTGGTGCTGGGTACCCCGCACCCGGTCAAGTTCCCCGAAGCGGTCGAGAAGGCCgcagt                                                                                                          >  6:242794/4‑145 (MQ=255)
                                           gccgccACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTggg                                                                                                       <  7:280170/149‑1 (MQ=255)
                                           gccgccACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTggg                                                                                                       >  8:280170/1‑149 (MQ=255)
                                            ccgccACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGc                                                                                                      <  7:384301/149‑1 (MQ=255)
                                              gccACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGc                                                                                                      >  2:89396/1‑147 (MQ=255)
                                              gccACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGc                                                                                                      >  8:233302/1‑147 (MQ=255)
                                              gccACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCaa                                                                                                    <  2:279899/149‑1 (MQ=255)
                                              gccACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCaa                                                                                                    <  7:150107/149‑1 (MQ=255)
                                                cACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGc                                                                                                                            <  7:133258/123‑1 (MQ=255)
                                                cACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGc                                                                                                                            >  8:133258/1‑123 (MQ=255)
                                                     gTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTg                                                                                             <  2:116398/149‑1 (MQ=255)
                                                     gTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCAGGTGTGGGCAAGGCGCTg                                                                                             >  1:372388/1‑149 (MQ=255)
                                                          gTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCt                                                                                        <  2:232879/149‑1 (MQ=255)
                                                           tGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTa                                                                                       >  2:186976/1‑149 (MQ=255)
                                                            gCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTAc                                                                                      >  5:320737/1‑149 (MQ=255)
                                                             cTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCt                                                                                              >  4:299245/1‑140 (MQ=255)
                                                             cTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCt                                                                                              <  3:299245/140‑1 (MQ=255)
                                                             cTCGACCCGCATACCGCAATAGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACAGGCCAATGGTGGTGCTGGGTACAGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCACGGCGCTGGAGCTAcc                                                                                     >  4:162163/1‑149 (MQ=255)
                                                              tCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGa                                                                                           <  6:76126/142‑1 (MQ=255)
                                                              tCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGa                                                                                           >  5:76126/1‑142 (MQ=255)
                                                                gACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCcgc                                                                                  >  8:130585/1‑149 (MQ=255)
                                                                 aCCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCcgcg                                                                                 >  6:190420/1‑149 (MQ=255)
                                                                          ccGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGc                                                                        <  5:190420/149‑1 (MQ=255)
                                                                           cGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCGCGGTACCCAGCAc                                                                                                                                                <  4:240961/76‑1 (MQ=255)
                                                                           cGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCGCGGTACCCAGCAc                                                                                                                                                >  3:240961/1‑76 (MQ=255)
                                                                           cGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCAGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCACGGAAGCGGTGGTGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGc                                                                        >  8:377309/1‑148 (MQ=255)
                                                                            gCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGa                                                                      >  8:101399/1‑149 (MQ=255)
                                                                             cAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGAc                                                                     <  7:130585/149‑1 (MQ=255)
                                                                                tCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGAcc                                                                    >  7:31544/1‑147 (MQ=255)
                                                                                tCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGAcc                                                                    <  8:31544/147‑1 (MQ=255)
                                                                                    cGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCa                                                              <  1:236468/149‑1 (MQ=255)
                                                                                     gTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGAcc                                                                    <  3:284738/142‑1 (MQ=255)
                                                                                     gTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGAcc                                                                    >  4:284738/1‑142 (MQ=255)
                                                                                       cAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGcc                                                           >  2:323222/1‑149 (MQ=255)
                                                                                        aaGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGGCATTGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGTAGCGGTGGAGAAGGCCGGGGGGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGcc                                                           <  5:242794/148‑1 (MQ=255)
                                                                                        aaGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAg                                                             <  1:232829/146‑1 (MQ=255)
                                                                                        aaGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAg                                                             >  2:232829/1‑146 (MQ=255)
                                                                                           gCTGCCCGCGTGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTg                                                        >  4:398482/1‑148 (MQ=255)
                                                                                           gCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGtt                                                                <  5:3639/140‑1 (MQ=255)
                                                                                           gCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGtt                                                                >  6:3639/1‑140 (MQ=255)
                                                                                               cccGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAaga                                                    <  7:101399/148‑1 (MQ=255)
                                                                                                ccGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGc                                                  >  2:225479/1‑149 (MQ=255)
                                                                                                ccGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGc                                                  >  2:391360/1‑149 (MQ=255)
                                                                                                   cgAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGtt                                               <  1:317645/149‑1 (MQ=255)
                                                                                                    gAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTg                                              >  6:172672/1‑149 (MQ=255)
                                                                                                       tGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCAc                                           >  4:227199/1‑149 (MQ=255)
                                                                                                         ccgccgCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACg                                          <  1:323222/148‑1 (MQ=255)
                                                                                                               cAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACGCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTgg                                   <  4:158553/149‑1 (MQ=255)
                                                                                                               cAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTgg                                   >  3:17575/1‑149 (MQ=255)
                                                                                                                      gACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGa                            >  8:210793/1‑149 (MQ=255)
                                                                                                                       acacGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCTGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGAc                           <  4:17575/149‑1 (MQ=255)
                                                                                                                           gCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGc                                             <  6:367163/127‑1 (MQ=255)
                                                                                                                           gCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGc                                             >  5:367163/1‑127 (MQ=255)
                                                                                                                           gCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCAGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTg                        >  1:116904/1‑148 (MQ=255)
                                                                                                                            ccAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGTGAATGCCGGTGTGGGGAAGGCGGTTGTGCTACCCGGGCACCTAAGCGTCCTTTTCAGCCGTGAAGATCGTTGCACGGTCCTGGCCAACGACCTGaa                      <  6:148645/149‑1 (MQ=255)
                                                                                                                            ccAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTg                        >  4:60962/1‑147 (MQ=255)
                                                                                                                             ttgaccgggtgCGCGGTACCCAGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAg                     >  4:262986/8‑149 (MQ=255)
                                                                                                                             cAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCaa                               >  3:14152/1‑139 (MQ=255)
                                                                                                                             cAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCaa                               <  4:14152/139‑1 (MQ=255)
                                                                                                                             cAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAg                     >  7:144618/1‑149 (MQ=255)
                                                                                                                              aaTGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAgg                    <  5:172672/149‑1 (MQ=255)
                                                                                                                                tggtggTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCt                  >  3:29747/1‑149 (MQ=255)
                                                                                                                                  gtggtgCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTg                 <  3:398482/148‑1 (MQ=255)
                                                                                                                                      tgCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAg            <  7:323962/149‑1 (MQ=255)
                                                                                                                                      tgCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAg            >  6:177985/1‑149 (MQ=255)
                                                                                                                                        cTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAggg          >  2:85685/1‑149 (MQ=255)
                                                                                                                                          gggTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCt        >  8:33440/1‑149 (MQ=255)
                                                                                                                                             tACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTg     <  7:136116/149‑1 (MQ=255)
                                                                                                                                             tACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTg     >  6:89164/1‑149 (MQ=255)
                                                                                                                                              aCCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGa                                                       <  6:384421/98‑1 (MQ=255)
                                                                                                                                              aCCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGa                                                       >  5:384421/1‑98 (MQ=255)
                                                                                                                                                cgcgCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTca  <  2:372388/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGTCAGCGACGAGCAAACCTGCGAGACCATCGCAGAAGTGTTCGCCGCCACCGGTGAAGTGCTCGACCCGCATACCGCAATCGGCGTCAAGGCTGCCCGCGAGTGCCGCCGCAGTCTGGACACGCCAATGGTGGTGCTGGGTACCGCGCACCCGGTCAAGTTCCCGGAAGCGGTGGAGAAGGCCGGTGTGGGCAAGGCGCTGGAGCTACCCGCGCACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCA  >  NC_002947/1677283‑1677575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑