Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,690,412 G→A 6.1% T140M (ACG→ATG) ‡ ydcS ← polyamine ABC transporter, periplasmic polyamine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,690,4120GA 6.1% 194.2 / 2.1 65T140M (ACG→ATG) ‡ydcSpolyamine ABC transporter, periplasmic polyamine‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (32/29);  new base A (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

GATCGGCCCGTCATAGGCTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATGCATCGCCC  >  NC_002947/1690264‑1690549
                                                                                                                                                    |                                                                                                                                         
gATCGGCCCGTCATAGGCTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCg                                                                                                                                           >  8:136093/1‑149 (MQ=255)
        cGTCATAGGCTTGCACGCGGCCCTTGTTGGGCTTGCCGGCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCACGCCGTac                                                                                                                                   <  2:140425/149‑1 (MQ=255)
          tCATAGGCTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCACGCCGTacca                                                                                                                                 <  1:155363/149‑3 (MQ=255)
            aTAGGCTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCt                                                                                                                               <  7:136093/149‑1 (MQ=255)
            aTAGGCTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGATCAAGCTGTTCGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGACGTacacat                                                                                                                               >  7:143198/1‑147 (MQ=255)
                gCTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCtt                                                                                                                           >  8:135214/1‑149 (MQ=255)
                 cTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGGGCGTGGTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGCTACGGCGTGCCGTACACCTGCTTg                                                                                                                          <  1:36546/149‑1 (MQ=255)
                        gcgGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACca                                                                                                                   <  7:135214/149‑1 (MQ=255)
                           gCCCTTTTTTGGCTTGCCTTCGGGCATGTTCTGCTGCTGGTTTACCACTCTCTAGCTGGTTGGCGCCTGCTTGTAGGTATCGGTGTTGTACAGCAGCACGTTTGGGCGCCTCTTGTTCGGCGTTCCGTACACCTGCTTGTCGACcactt                                                                                                                <  2:343521/149‑3 (MQ=255)
                             ccTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACAtcc                                                                                                              >  8:175948/1‑147 (MQ=255)
                             ccTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATAc                                                                                                              >  1:202229/1‑149 (MQ=255)
                             ccTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATAc                                                                                                              >  7:1727/1‑149 (MQ=255)
                               ttGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCa                                                                                                            >  4:1084/1‑149 (MQ=255)
                                 gTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCa                                                                                                            <  3:88837/147‑1 (MQ=255)
                                 gTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCa                                                                                                            <  3:378744/147‑1 (MQ=255)
                                  tttGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGc                                                                                                          >  2:69996/1‑148 (MQ=255)
                                  tttGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGc                                                                                                          <  2:202229/148‑1 (MQ=255)
                                          gCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGtt                                                                                                   <  2:207580/147‑1 (MQ=255)
                                          gCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTc                                                                                                  <  3:269083/148‑1 (MQ=255)
                                          gCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCt                                                                                                 >  3:175502/1‑149 (MQ=255)
                                              tCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAg                                                                                             >  4:245520/1‑149 (MQ=255)
                                                  gCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGAACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGACGACGACATACCAGCTACCGTTCTGAAGgcgc                                                                                         >  3:230528/1‑149 (MQ=255)
                                                    aGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCgg                                                                                       <  7:154240/149‑1 (MQ=255)
                                                     ggTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCggg                                                                                      <  1:69996/149‑1 (MQ=255)
                                                            cGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATg                                                                                <  3:1084/148‑1 (MQ=255)
                                                                 cGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTc                                                                          <  3:245520/149‑1 (MQ=255)
                                                                 cGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTc                                                                          >  6:201979/1‑149 (MQ=255)
                                                                  gAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGctct                                                                            >  3:42218/1‑146 (MQ=255)
                                                                  gAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGctct                                                                            <  4:42218/146‑1 (MQ=255)
                                                                  gAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTc                                                                          >  7:379607/1‑148 (MQ=255)
                                                                  gAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTc                                                                          <  8:379607/148‑1 (MQ=255)
                                                                    acacCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGAta                                                                                >  1:192255/1‑139 (MQ=255)
                                                                    acacCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGAta                                                                                <  2:192255/140‑2 (MQ=255)
                                                                        cacGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTgg                                                                   >  3:54144/1‑149 (MQ=255)
                                                                        cacGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTgg                                                                   <  5:201979/149‑1 (MQ=255)
                                                                        cacGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTgg                                                                   >  5:65145/1‑149 (MQ=255)
                                                                        cacGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTT‑CAGTTggg                                                                  >  3:359698/1‑149 (MQ=255)
                                                                             tCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGAt                                                               >  6:233597/1‑148 (MQ=255)
                                                                                aGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAgg                                                           >  4:380537/1‑149 (MQ=255)
                                                                                 gCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAggg                                                          <  6:65145/149‑1 (MQ=255)
                                                                                       gggCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAgtgt                                                    >  6:90525/1‑149 (MQ=255)
                                                                                        ggggcCTGCTTGTCGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAgtgt                                                    <  4:230528/145‑1 (MQ=255)
                                                                                        ggCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGtt                                                   <  4:175502/149‑1 (MQ=255)
                                                                                         gcgcCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGtt                                                   >  2:114137/1‑148 (MQ=255)
                                                                                             cTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCg                                              >  4:113588/1‑149 (MQ=255)
                                                                                                 ttGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTg                                          >  7:366607/1‑149 (MQ=255)
                                                                                                    aaGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTAc                                       <  3:113588/149‑1 (MQ=255)
                                                                                                      ggTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTAcgc                                     >  5:317947/1‑149 (MQ=255)
                                                                                                             gtgtTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGcc                              >  3:15657/1‑149 (MQ=255)
                                                                                                              ggttgtACAGCAGCACGTTTGGCCCACACTGGTACGGCGTGACGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATTCTCTTCCATTTGGGGATCAGGGAAGTGTTGATCGGCTGTACGCGCtt                                 <  8:143198/144‑1 (MQ=255)
                                                                                                                   tACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTAcgc                                     >  5:33843/1‑136 (MQ=255)
                                                                                                                   tACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTAcgc                                     <  6:33843/136‑1 (MQ=255)
                                                                                                                   tACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTATTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCGGCAAt                        >  4:214550/1‑149 (MQ=255)
                                                                                                                      agcagcACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATAAGGGCAGTGTTGa                                                 <  4:99038/121‑1 (MQ=255)
                                                                                                                      agcagcACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATAAGGGCAGTGTTGa                                                 >  3:99038/1‑121 (MQ=255)
                                                                                                                        cagcaCGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTT‑CAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCg                  <  4:359698/149‑1 (MQ=255)
                                                                                                                                  ggCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATg          <  4:15657/148‑1 (MQ=255)
                                                                                                                                   gCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGATCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATGCa        >  1:109574/1‑149 (MQ=255)
                                                                                                                                     ccccACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAgtgt                                                    <  2:99255/103‑1 (MQ=255)
                                                                                                                                     ccccACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAgtgt                                                    >  1:99255/1‑103 (MQ=255)
                                                                                                                                     ccccACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATGCATc      <  8:1727/149‑1 (MQ=255)
                                                                                                                                       ccACTGGTACGGCGTGCCGTACGCATGATTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATGCATCGc    >  7:61222/1‑149 (MQ=255)
                                                                                                                                           gtgTACGGCACGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATGCATCGccc  >  2:347816/3‑147 (MQ=255)
                                                                                                                                           gtgTACGGCACGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATGCATCGccc  >  2:105277/3‑147 (MQ=255)
                                                                                                                                                cGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGAt           <  6:349386/133‑1 (MQ=255)
                                                                                                                                                cGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGAt           >  5:349386/1‑133 (MQ=255)
                                                                                                                                                    |                                                                                                                                         
GATCGGCCCGTCATAGGCTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATGCATCGCCC  >  NC_002947/1690264‑1690549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑