Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,690,413 T→C 6.1% T140A (ACG→GCG) ‡ ydcS ← polyamine ABC transporter, periplasmic polyamine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,690,4130TC 6.1% 190.6 / 2.3 65T140A (ACG→GCG) ‡ydcSpolyamine ABC transporter, periplasmic polyamine‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (32/29);  new base C (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

CGTCATAGGCTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATGCATCGCCC  >  NC_002947/1690272‑1690549
                                                                                                                                             |                                                                                                                                        
cGTCATAGGCTTGCACGCGGCCCTTGTTGGGCTTGCCGGCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCACGCCGTac                                                                                                                                   <  2:140425/149‑1 (MQ=255)
  tCATAGGCTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCACGCCGTacca                                                                                                                                 <  1:155363/149‑3 (MQ=255)
    aTAGGCTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCt                                                                                                                               <  7:136093/149‑1 (MQ=255)
    aTAGGCTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGATCAAGCTGTTCGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGACGTacacat                                                                                                                               >  7:143198/1‑147 (MQ=255)
        gCTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCtt                                                                                                                           >  8:135214/1‑149 (MQ=255)
         cTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGGGCGTGGTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGCTACGGCGTGCCGTACACCTGCTTg                                                                                                                          <  1:36546/149‑1 (MQ=255)
                gcgGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACca                                                                                                                   <  7:135214/149‑1 (MQ=255)
                   gCCCTTTTTTGGCTTGCCTTCGGGCATGTTCTGCTGCTGGTTTACCACTCTCTAGCTGGTTGGCGCCTGCTTGTAGGTATCGGTGTTGTACAGCAGCACGTTTGGGCGCCTCTTGTTCGGCGTTCCGTACACCTGCTTGTCGACcactt                                                                                                                <  2:343521/149‑3 (MQ=255)
                     ccTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACAtcc                                                                                                              >  8:175948/1‑147 (MQ=255)
                     ccTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATAc                                                                                                              >  1:202229/1‑149 (MQ=255)
                     ccTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATAc                                                                                                              >  7:1727/1‑149 (MQ=255)
                       ttGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCa                                                                                                            >  4:1084/1‑149 (MQ=255)
                         gTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCa                                                                                                            <  3:88837/147‑1 (MQ=255)
                         gTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCa                                                                                                            <  3:378744/147‑1 (MQ=255)
                          tttGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGc                                                                                                          <  2:202229/148‑1 (MQ=255)
                          tttGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGc                                                                                                          >  2:69996/1‑148 (MQ=255)
                                  gCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGtt                                                                                                   <  2:207580/147‑1 (MQ=255)
                                  gCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTc                                                                                                  <  3:269083/148‑1 (MQ=255)
                                  gCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCt                                                                                                 >  3:175502/1‑149 (MQ=255)
                                      tCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAg                                                                                             >  4:245520/1‑149 (MQ=255)
                                          gCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGAACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGACGACGACATACCAGCTACCGTTCTGAAGgcgc                                                                                         >  3:230528/1‑149 (MQ=255)
                                            aGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCgg                                                                                       <  7:154240/149‑1 (MQ=255)
                                             ggTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCggg                                                                                      <  1:69996/149‑1 (MQ=255)
                                                    cGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATg                                                                                <  3:1084/148‑1 (MQ=255)
                                                         cGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTc                                                                          >  6:201979/1‑149 (MQ=255)
                                                         cGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTc                                                                          <  3:245520/149‑1 (MQ=255)
                                                          gAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGctct                                                                            <  4:42218/146‑1 (MQ=255)
                                                          gAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGctct                                                                            >  3:42218/1‑146 (MQ=255)
                                                          gAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTc                                                                          >  7:379607/1‑148 (MQ=255)
                                                          gAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTc                                                                          <  8:379607/148‑1 (MQ=255)
                                                            acacCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGAta                                                                                >  1:192255/1‑139 (MQ=255)
                                                            acacCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGAta                                                                                <  2:192255/140‑2 (MQ=255)
                                                                cacGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTgg                                                                   >  3:54144/1‑149 (MQ=255)
                                                                cacGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTgg                                                                   <  5:201979/149‑1 (MQ=255)
                                                                cacGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTgg                                                                   >  5:65145/1‑149 (MQ=255)
                                                                cacGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTT‑CAGTTggg                                                                  >  3:359698/1‑149 (MQ=255)
                                                                     tCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGAt                                                               >  6:233597/1‑148 (MQ=255)
                                                                        aGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAgg                                                           >  4:380537/1‑149 (MQ=255)
                                                                         gCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAggg                                                          <  6:65145/149‑1 (MQ=255)
                                                                               gggCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAgtgt                                                    >  6:90525/1‑149 (MQ=255)
                                                                                ggggcCTGCTTGTCGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAgtgt                                                    <  4:230528/145‑1 (MQ=255)
                                                                                ggCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGtt                                                   <  4:175502/149‑1 (MQ=255)
                                                                                 gcgcCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGtt                                                   >  2:114137/1‑148 (MQ=255)
                                                                                     cTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCg                                              >  4:113588/1‑149 (MQ=255)
                                                                                         ttGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTg                                          >  7:366607/1‑149 (MQ=255)
                                                                                            aaGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTAc                                       <  3:113588/149‑1 (MQ=255)
                                                                                              ggTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTAcgc                                     >  5:317947/1‑149 (MQ=255)
                                                                                                     gtgtTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGcc                              >  3:15657/1‑149 (MQ=255)
                                                                                                      ggttgtACAGCAGCACGTTTGGCCCACACTGGTACGGCGTGACGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATTCTCTTCCATTTGGGGATCAGGGAAGTGTTGATCGGCTGTACGCGCtt                                 <  8:143198/144‑1 (MQ=255)
                                                                                                           tACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTAcgc                                     >  5:33843/1‑136 (MQ=255)
                                                                                                           tACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTAcgc                                     <  6:33843/136‑1 (MQ=255)
                                                                                                           tACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTATTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCGGCAAt                        >  4:214550/1‑149 (MQ=255)
                                                                                                              agcagcACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATAAGGGCAGTGTTGa                                                 >  3:99038/1‑121 (MQ=255)
                                                                                                              agcagcACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATAAGGGCAGTGTTGa                                                 <  4:99038/121‑1 (MQ=255)
                                                                                                                cagcaCGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTT‑CAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCg                  <  4:359698/149‑1 (MQ=255)
                                                                                                                          ggCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATg          <  4:15657/148‑1 (MQ=255)
                                                                                                                           gCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGATCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATGCa        >  1:109574/1‑149 (MQ=255)
                                                                                                                             ccccACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAgtgt                                                    >  1:99255/1‑103 (MQ=255)
                                                                                                                             ccccACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAgtgt                                                    <  2:99255/103‑1 (MQ=255)
                                                                                                                             ccccACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATGCATc      <  8:1727/149‑1 (MQ=255)
                                                                                                                               ccACTGGTACGGCGTGCCGTACGCATGATTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATGCATCGc    >  7:61222/1‑149 (MQ=255)
                                                                                                                                   gtgTACGGCACGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATGCATCGccc  >  2:347816/3‑147 (MQ=255)
                                                                                                                                   gtgTACGGCACGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATGCATCGccc  >  2:105277/3‑147 (MQ=255)
                                                                                                                                        cGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGAt           <  6:349386/133‑1 (MQ=255)
                                                                                                                                        cGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGAt           >  5:349386/1‑133 (MQ=255)
                                                                                                                                             |                                                                                                                                        
CGTCATAGGCTTGCACGCGGCCCTTGTTTGGCTTGCCGTCGGGCAGGTTCTGCGGCTCGAACACCACGCTCCAGCTGGTGGGCGCCTGCTTGAAGGTATCGGTGTTGTACAGCAGCACGTTTGGCCCCCACTGGTACGGCGTGCCGTACACCTGCTTGTCGACCACATACCAGCTACCGTTCTGCAGGCGCGGGTCGATGCTCTTCCAGTTGGGGATCAGGGCAGTGTTGATCGGCTGTACGCGCTTGCCTGCAATCAGCCGCAGCGATGCATCGCCC  >  NC_002947/1690272‑1690549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑