Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,706,340	C→A	8.7%	A135A (GCC→GCA) ‡	PP_1500 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,706,340	0	C	A	8.7%	206.8 / 9.1	69	A135A (GCC→GCA) ‡	PP_1500	lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (32/31);  new base A (2/4);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01

ACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCGGCCA  >  NC_002947/1706198‑1706488                                                                                                                                               |                                                                                                                                                     aCCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGGCTg                                                                                                                                                <  6:189785/149‑1 (MQ=255)   cTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTgca                                                                                                                                              >  4:281606/1‑149 (MQ=255)   cTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTg                                                                                                                                                >  5:301823/1‑147 (MQ=255)   cTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGGCTgca                                                                                                                                              <  1:239236/149‑1 (MQ=255)    tGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTgcag                                                                                                                                             >  6:7255/1‑149 (MQ=255)    tGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTgcag                                                                                                                                             >  6:33732/1‑149 (MQ=255)    tGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACAGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGAAATTGCTGCAGCCATGGCCTGGCTgctg                                                                                                                                             >  3:246955/1‑147 (MQ=255)     gCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTgcag                                                                                                                                             >  1:396654/1‑148 (MQ=255)      ccAGGGCACCTGGGGGGTAGCTACTGGGTGCCGGAGCAGGGCTACCAGGTGCTGATGATGGATTACCGCGGCTATTGCTTGTCTCAGGGCCAGCCGAGCCTGACGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTgcagca                                                                                                                                           <  1:231701/149‑1 (MQ=255)      ccAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGTCTgcag                                                                                                                                             <  8:256883/147‑1 (MQ=255)                  gggggTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCCGCAGGCGACCGAgg                                                                                                                                >  4:48077/1‑148 (MQ=255)                  gggggTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAgg                                                                                                                                <  3:129381/148‑1 (MQ=255)                  gggggTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAg                                                                                                                                 <  4:335654/147‑1 (MQ=255)                  gggggTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGt                                                                                                                               <  6:278590/149‑1 (MQ=255)                      gTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAg                                                                                                                           <  5:234452/149‑1 (MQ=255)                      gTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAg                                                                                                                           <  3:281606/149‑1 (MQ=255)                         gCTACTGGCTTCCGGAGCAGTGCTACCATGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGAAATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGc                                                                                                                        <  4:246955/149‑1 (MQ=255)                            aCTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAg                                                                                                                     >  3:144515/1‑149 (MQ=255)                                    ccGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTgg                                                                                                              >  7:147594/1‑148 (MQ=255)                                     cGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTa                                                                                                            <  5:33732/149‑1 (MQ=255)                                      ggAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGGTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGc                                                                                                                    <  8:22259/140‑1 (MQ=255)                                      ggAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGc                                                                                                                    >  7:22259/1‑140 (MQ=255)                                       gAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTAct                                                                                                          >  6:9535/1‑149 (MQ=255)                                        aGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTActg                                                                                                         <  5:7255/149‑1 (MQ=255)                                              gCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTgggg                                                                                                   <  5:135487/149‑1 (MQ=255)                                                     ggTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGcc                                                                                            >  8:468/1‑149 (MQ=255)                                                     ggTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAACCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGcc                                                                                            >  1:286943/1‑149 (MQ=255)                                                      gTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCt                                                                                           >  8:262744/1‑149 (MQ=255)                                                        gCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTgg                                                                                         >  3:136897/1‑149 (MQ=255)                                                         cTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTggg                                                                                        <  5:9535/149‑1 (MQ=255)                                                         cTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTgg                                                                                         <  4:144515/148‑1 (MQ=255)                                                            atgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCgg                                                                                     >  5:177649/1‑149 (MQ=255)                                                              gatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTg                                                                                   >  3:114197/1‑149 (MQ=255)                                                                   aTTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATg                                                                              >  5:137937/1‑149 (MQ=255)                                                                      aCCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATg                                                                              >  5:129118/1‑146 (MQ=255)                                                                      aCCGCGGATATGGCTGGTCTCAGGTCCAGGCGAGCCTGCCGGAGGTGTACCTGGACATTGCTGCAGCCATGGGCTGGCTGCAGCAGGCGGCCGAGGTCAAGGGCAAGGCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATg                                                                              <  6:129118/146‑1 (MQ=255)                                                                       ccGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCca                                                                          >  2:376518/1‑149 (MQ=255)                                                                         gcgGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcatt                                                                        <  4:39891/149‑1 (MQ=255)                                                                            gCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcattcat                                                                     <  7:468/149‑1 (MQ=255)                                                                                      gTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTggcg                                                            <  2:286943/148‑1 (MQ=255)                                                                                       tctcAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTggcggc                                                          >  5:371911/1‑149 (MQ=255)                                                                                       tctcAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTggcggc                                                          >  2:390656/1‑149 (MQ=255)                                                                                         tcAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGccc                                                        >  2:261578/1‑149 (MQ=255)                                                                                         tcAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGcc                                                         >  4:71440/1‑148 (MQ=255)                                                                                              gCCAGCCGAGCCTGCCGGAGGTGTACCAGGACAGTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCAcccc                                                   <  3:48077/149‑1 (MQ=255)                                                                                                cAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGa                                                 >  5:384994/1‑149 (MQ=255)                                                                                                cAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGa                                                 <  4:357538/149‑1 (MQ=255)                                                                                                 aGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCTag                                                >  4:28505/1‑149 (MQ=255)                                                                                                 aGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGag                                                >  1:57836/1‑149 (MQ=255)                                                                                                  gCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGagc                                               <  2:57836/149‑1 (MQ=255)                                                                                                   ccGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGagca                                              <  1:390656/149‑1 (MQ=255)                                                                                                   ccGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTACTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGag                                                >  6:354831/1‑147 (MQ=255)                                                                                                        ccTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTc                                         >  7:358172/1‑149 (MQ=255)                                                                                                            ccGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcattcatt                                                                    >  4:62708/1‑118 (MQ=255)                                                                                                            ccGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcattcatt                                                                    <  3:62708/118‑1 (MQ=255)                                                                                                            ccGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAgcg                                     <  3:71440/149‑1 (MQ=255)                                                                                                            ccGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAg                                       <  1:376518/147‑1 (MQ=255)                                                                                                                   tgtACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAgc                              <  6:371911/149‑1 (MQ=255)                                                                                                                     tACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAgcgc                            <  8:229283/149‑1 (MQ=255)                                                                                                                       ccAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGcc                           <  8:147594/148‑1 (MQ=255)                                                                                                                       ccAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGGCAAGGGCAAGCCTCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGcc                           <  4:206485/148‑1 (MQ=255)                                                                                                                         atgACATTGATGCAGCCATGGCCTGGCTTCAGAAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGTGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGTCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTg                        <  2:396654/147‑1 (MQ=255)                                                                                                                              cctgctgcAGCCAGGCCATGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGgtgt                    >  8:350836/3‑148 (MQ=255)                                                                                                                              cctgctgcAGCCAGGCCATGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGtt                   <  7:350836/147‑1 (MQ=255)                                                                                                                                  ctgcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGAt               <  6:384994/149‑1 (MQ=255)                                                                                                                                  ctgcAGCCAGGCCATGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGAt               >  6:210733/1‑149 (MQ=255)                                                                                                                                   tgcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg              >  3:399882/1‑149 (MQ=255)                                                                                                                                        ccATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGtgtg         >  3:23526/1‑149 (MQ=255)                                                                                                                                         cATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGc        <  6:137937/149‑1 (MQ=255)                                                                                                                                               cTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCGGCCa  <  6:177649/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                     ACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCGGCCA  >  NC_002947/1706198‑1706488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑