Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,710,161	T→A	9.2%	A260A (GCT→GCA)	ppc →	phosphoenolpyruvate carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,710,161	0	T	A	9.2%	173.8 / 6.1	62	A260A (GCT→GCA)	ppc	phosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base T (30/26);  new base A (2/4);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

ACTGTGGCATGCCATCCCCAGCCACCTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑TGCGCAAGCAGGTTGGCGACAGTGCCGAACCCTACC  >  NC_002947/1710014‑1710309                                                                                                                                                    |                                                                                                                                                       aCTGTGGCATGCCATCCCCAGCCACCTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTg                                                                                                                                                       >  3:75389/1‑149 (MQ=255)     tgGCATGCCATCCCCAGCCACCTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGt                                                                                                                                                   >  8:9032/1‑149 (MQ=255)         aTGCCATCCCCAGCCACCTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCAGCCGTCAca                                                                                                                                               >  4:35387/1‑148 (MQ=255)            ccATCCCCAGCCACCTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCAGCCGTCAcattg                                                                                                                                            <  3:35387/149‑5 (MQ=255)            ccATCCCCAGCCACCTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCAGCCGTCAcatt                                                                                                                                             <  5:132199/148‑4 (MQ=255)              aTCCCCAGCCACCTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCAGCCGTCAcattggg                                                                                                                                          <  8:30284/149‑7 (MQ=255)                  ccAGCCACCTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTa                                                                                                                                      >  8:282485/1‑149 (MQ=255)                   cAGCCACCTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTa                                                                                                                                      >  8:59210/1‑148 (MQ=255)                   cAGCCACCTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTAc                                                                                                                                     >  5:16355/1‑149 (MQ=255)                   cAGCCACCTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCAGCCGTCAcattg                                                                                                                                            >  4:155098/1‑138 (MQ=255)                   cAGCCACCTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCAGCCGTCAcattg                                                                                                                                            <  3:155098/142‑5 (MQ=255)                       cACCTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCct                                                                                                                                 <  3:70936/149‑1 (MQ=255)                         ccTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCctgc                                                                                                                               >  1:29901/1‑149 (MQ=255)                            gcgcAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTgg                                                                                                                            >  8:362931/1‑149 (MQ=255)                                      gACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGa                                                                                                                   <  5:128624/148‑1 (MQ=255)                                      gACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGAt                                                                                                                  <  6:249046/149‑1 (MQ=255)                                       aCAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATg                                                                                                                 <  7:9032/149‑1 (MQ=255)                                           ggCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGgccg                                                                                                             >  2:39874/1‑149 (MQ=255)                                                  ctgGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGAcc                                                                                                       <  4:96612/148‑1 (MQ=255)                                                  ctgGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGAc                                                                                                        <  7:362931/147‑1 (MQ=255)                                                  ctgGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGAc                                                                                                        <  2:29901/147‑1 (MQ=255)                                                  ctgGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGAc                                                                                                        <  3:327846/147‑1 (MQ=255)                                                      aGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTc                                                                                                 <  1:165460/149‑1 (MQ=255)                                                            cTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTgcgc                                                                                           <  4:75389/149‑1 (MQ=255)                                                                cctgcgcctgcCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACa                                                                                       >  6:47337/1‑149 (MQ=255)                                                                 ctgcgcctgcCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGGGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGAc                                                                                                        >  1:333784/1‑132 (MQ=255)                                                                 ctgcgcctgcCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGAc                                                                                                        <  2:333784/132‑1 (MQ=255)                                                                      cctgcCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATg                                                                                 >  1:218105/1‑149 (MQ=255)                                                                      cctgcCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATg                                                                                 <  3:119506/149‑1 (MQ=255)                                                                       ctgcCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATg                                                                                 >  1:68143/1‑148 (MQ=255)                                                                       ctgcCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATg                                                                                 >  3:45335/1‑148 (MQ=255)                                                                                   gCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGa                                                                    <  1:39874/149‑1 (MQ=255)                                                                                    ccGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAg                                                                   >  6:402015/1‑149 (MQ=255)                                                                                        gcCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTg                                                                            <  2:140304/136‑1 (MQ=255)                                                                                        gcCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTg                                                                            >  1:140304/1‑136 (MQ=255)                                                                                           gATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCa                                                            <  7:59210/149‑1 (MQ=255)                                                                                                  tttGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATgcagca                                                     <  4:45335/149‑1 (MQ=255)                                                                                                              aTGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGacac                                         >  1:285988/1‑149 (MQ=255)                                                                                                              aTGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGacac                                         >  1:280352/1‑149 (MQ=255)                                                                                                                  gtgggGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑Tg                                    >  8:188268/1‑149 (MQ=255)                                                                                                                   tgggGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑Tgc                                   >  7:32244/1‑149 (MQ=255)                                                                                                                    ggggACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑Tgcg                                  >  6:343235/1‑149 (MQ=255)                                                                                                                       gACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑Tgcgc                                 >  1:201804/1‑147 (MQ=255)                                                                                                                           gTGATGGCAACCCCAATGTGACGGCTGCCGTCCCCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TCTTCCTGCGCGACATCGATGCACTGGCCGCCGAGATGTCCATGCAGCAGGCCAACGACACCC‑TGCGCAAGCAg                           >  7:212618/1‑149 (MQ=255)                                                                                                                            tGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGAGCGACATCGATGCACTGGCAGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑TGCGCAAGCAgg                          >  7:256013/1‑149 (MQ=255)                                                                                                                             gATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑Tgcgc                                 <  8:156966/141‑1 (MQ=255)                                                                                                                             gATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑Tgcgc                                 >  7:156966/1‑141 (MQ=255)                                                                                                                              aTGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGAt                                                                                                                  >  2:347902/1‑61 (MQ=255)                                                                                                                              aTGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGAt                                                                                                                  <  1:347902/61‑1 (MQ=255)                                                                                                                               tGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑TGCGCAAGCAGGTTg                       <  6:132156/149‑1 (MQ=255)                                                                                                                                  cAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑TGCGCAAGCAGGTTGGc                     >  3:1487/1‑148 (MQ=255)                                                                                                                                     ccccAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑TGCGCAAGCAGGTTGGCGACa                 <  2:52988/149‑1 (MQ=255)                                                                                                                                      cccAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCTCTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑TGCGCAAGCAGGTTg                       >  7:325290/1‑142 (MQ=255)                                                                                                                                      cccAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCTCTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑TGCGCAAGCAGGTTg                       <  8:325290/142‑1 (MQ=255)                                                                                                                                      cccAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑TGCGCAAGCAGGTTGGCGACAg                >  4:288391/1‑149 (MQ=255)                                                                                                                                        cAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACCAGGTTACTGCGCGACATCGATGCACTGGCCGCCGAGTTGTACATGCCGCACGCCAAAGCCACCC‑AGCGCAAGCCGGTTGGCGACact               >  1:320482/1‑147 (MQ=255)                                                                                                                                        cAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGa                                                                    <  7:143648/96‑1 (MQ=255)                                                                                                                                        cAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCCACATCGATGCACTGGCCGCCGa                                                                    >  8:143648/1‑96 (MQ=255)                                                                                                                                                cGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCCTTGCGCAAGCAGGTTGGCGACAGTGCCGAAcc       <  1:35932/149‑1 (MQ=255)                                                                                                                                                cGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑TGCGCAAGCAGGTTGGCGACAGTGCCGAAccc      <  3:385991/149‑1 (MQ=255)                                                                                                                                                  gCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑TGCGCAAGCAGGTTGGCGACAGTGCCGAACCCTa    <  2:280352/149‑1 (MQ=255)                                                                                                                                                  gCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑TGCGCAAGCAGGTTGGCGACAGTGCCGAACCCTa    <  2:285988/149‑1 (MQ=255)                                                                                                                                                    tGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑TGCGCAAGCAGGTTGGCGACAGTGCCGAACCCTAcc  >  1:357681/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                       ACTGTGGCATGCCATCCCCAGCCACCTGCGCAAGGTCGACAAGGCCCTGCTGGAGGCCACTGGCCTGCGCCTGCCGCTGGAGGCCGCGCCGATCCGCTTTGCCTCGTGGATGGGTGGGGACCGTGATGGCAACCCCAATGTGACGGCTGCCGTCACCCGCGAAGTACTCCTGCTGGCGCGCTGGATGGCCGCCGACC‑TGTTCCTGCGCGACATCGATGCACTGGCCGCCGAGTTGTCCATGCAGCAGGCCAACGACACCC‑TGCGCAAGCAGGTTGGCGACAGTGCCGAACCCTACC  >  NC_002947/1710014‑1710309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑