Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,710,935 T→C 5.7% L518L (CTT→CTC)  ppc → phosphoenolpyruvate carboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,710,9350TC 5.7% 206.6 / 2.2 70L518L (CTT→CTC) ppcphosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base T (34/32);  new base C (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

ATCTCCATGGCCGGTGCCGCCTCGGATGTACTGGCGGTGCAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGCTCAGGAAAACCTGGTGCGTATCTGTGCCGAGCA  >  NC_002947/1710789‑1711081
                                                                                                                                                  |                                                                                                                                                  
aTCTCCATGGCCGGTGCCGCCTCGGATGTACTGGCGGTGCAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTgg                                                                                                                                                  <  8:219528/149‑1 (MQ=255)
    ccATGGCCGGTGCCGCCTCGGATGTACTGGCGGTGCAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTg                                                                                                                                              >  5:261300/1‑149 (MQ=255)
       tGGCCGGTGCCGCCTCGGATGTACTGGCGGTGCAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGCCAACGCCGGCCCGGTCATGCAGCGCCTGCTTGCCCTGCCg                                                                                                                                           >  8:302034/1‑149 (MQ=255)
           cGGTGCCGCCTCGGATGTTCTGGCGGTGCAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGAGCGTGGTGCCACTGTTCGAGACCCTGGCCTACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGATTGTCATGCCggtc                                                                                                                                        >  4:16981/1‑146 (MQ=255)
           cGGTGCCGCCTCGGATGTACTGGCGGTTCAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGACTGCCAGGTCATGCAGCGCCTGCTTGGCCTGCCTGGc                                                                                                                                        >  4:11391/1‑148 (MQ=255)
                  gccTCGGATGTACTGGCGGTGCAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACcgcgc                                                                                                                                <  8:216712/149‑1 (MQ=255)
                  gccTCGGATGTACTGGCGGTGCAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACcgcgc                                                                                                                                <  2:350056/149‑1 (MQ=255)
                  gccTCGGATGTACTGGCGGTGCAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACcgcgc                                                                                                                                >  2:27084/1‑149 (MQ=255)
                   ccTCGGATGTACTGGCGGTGCAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACcgcgcg                                                                                                                               <  6:261300/149‑1 (MQ=255)
                             aCTGGCGGTGCAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTCCTTGGCCTGCCGGTCTACCGCGCGGGCCTGcgcg                                                                                                                     >  6:124333/1‑149 (MQ=255)
                                 gCGGTGTAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTg                                                                                                                         <  2:145241/141‑1 (MQ=255)
                                 gCGGTGTAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTg                                                                                                                         >  1:145241/1‑141 (MQ=255)
                                 gCGGTGCAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGTGCGGccc                                                                                                                 <  2:89005/149‑1 (MQ=255)
                                        aaCTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAgg                                                                                                            >  1:299973/1‑147 (MQ=255)
                                         aCTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCGTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAg                                                                                                         <  6:298419/149‑1 (MQ=255)
                                             ttGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGtga                                                                                                      <  6:389202/148‑1 (MQ=255)
                                                cTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTCGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTgatgat                                                                                                  >  3:76364/1‑149 (MQ=255)
                                                  cAAGGAAGCCGGCCTGACCAGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCg                                                                                                >  4:250220/1‑149 (MQ=255)
                                                   aaGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAgg                                                                                                            >  4:136816/1‑136 (MQ=255)
                                                   aaGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAgg                                                                                                            <  3:136816/136‑1 (MQ=255)
                                                   aaGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCgg                                                                                               >  2:287588/1‑149 (MQ=255)
                                                   aaGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCgg                                                                                               <  2:299973/149‑1 (MQ=255)
                                                   aaGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTCGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCgg                                                                                               <  4:76364/149‑1 (MQ=255)
                                                     ggAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGc                                                                                              >  3:124203/1‑148 (MQ=255)
                                                       aaGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTAc                                                                                           >  5:65614/1‑149 (MQ=255)
                                                         gCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTc                                                                                         <  1:287588/149‑1 (MQ=255)
                                                          ccGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTcc                                                                                        >  2:377745/1‑149 (MQ=255)
                                                          ccGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTcc                                                                                        >  4:23544/1‑149 (MQ=255)
                                                           cGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGACCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTcc                                                                                        >  1:89803/1‑148 (MQ=255)
                                                            ggCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGa                                                                                      >  7:99782/1‑149 (MQ=255)
                                                                      gCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAg                                                                            >  5:142350/1‑149 (MQ=255)
                                                                      gCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAg                                                                            <  6:66713/149‑1 (MQ=255)
                                                                          cATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACg                                                                        >  7:268474/1‑149 (MQ=255)
                                                                               gcgTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGTTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGc                                                                   <  5:124333/149‑1 (MQ=255)
                                                                               gcgTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTCGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTc                                                                                   >  8:260693/1‑133 (MQ=255)
                                                                               gcgTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTCGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTc                                                                                   <  7:260693/133‑1 (MQ=255)
                                                                                    gtgCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGcaccac                                                              <  6:65614/149‑1 (MQ=255)
                                                                                       ccACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCgc                                                           <  3:250220/149‑1 (MQ=255)
                                                                                        cACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCgct                                                          >  2:241451/1‑149 (MQ=255)
                                                                                          cTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCgctgc                                                        <  1:241451/149‑1 (MQ=255)
                                                                                             ttCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCtgctgc                                                     <  8:268474/149‑1 (MQ=255)
                                                                                             ttCGACACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCgctgct                                                       <  8:343475/147‑1 (MQ=255)
                                                                                               cGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGcc                                                    <  1:377745/148‑1 (MQ=255)
                                                                                                gagaCCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGCTCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTg                                                  >  6:392650/1‑149 (MQ=255)
                                                                                                 agaCCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTgg                                                 >  7:249592/1‑149 (MQ=255)
                                                                                                 agaCCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTgg                                                 >  6:78359/1‑149 (MQ=255)
                                                                                                  gaCCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGGGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGGCAAg                                                                            <  1:392200/121‑1 (MQ=255)
                                                                                                  gaCCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCATGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAACAGATGATCGGCTACTCCGATTCGGCCAAg                                                                            >  2:392200/1‑121 (MQ=255)
                                                                                                     ccTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGcaccac                                                              >  8:243640/1‑132 (MQ=255)
                                                                                                     ccTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGcaccac                                                              <  7:243640/132‑1 (MQ=255)
                                                                                                     ccTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGgcgc                                             >  4:78913/1‑149 (MQ=255)
                                                                                                     ccTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGgcgc                                             >  4:173502/1‑149 (MQ=255)
                                                                                                     ccTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGgcg                                              >  3:383442/1‑148 (MQ=255)
                                                                                                               cTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGc                                   >  6:207887/1‑149 (MQ=255)
                                                                                                                  gACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGCTc                                 <  8:99782/148‑1 (MQ=255)
                                                                                                                     aaCGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGc                                                                   >  7:194668/1‑111 (MQ=255)
                                                                                                                     aaCGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGc                                                                   <  8:194668/111‑1 (MQ=255)
                                                                                                                            gCCCGGTCATGCAGCGCCTCCTTGGCCTGCCGGGCTACAGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTAGTCGGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGCTCAGGaaagcgtg                      >  8:298170/1‑144 (MQ=255)
                                                                                                                             cccGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCGGTACCGGGCTCAGGAAAACCTgg                     <  3:78913/149‑1 (MQ=255)
                                                                                                                             cccGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCGGTACCGGGCTCAGGAAAACCTgg                     <  3:173502/149‑1 (MQ=255)
                                                                                                                                ggTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGCTCAGGAAAACCTGGTGc                  <  4:383442/149‑1 (MQ=255)
                                                                                                                                 gTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGCTCAGGAAAACCTGGTGCg                 >  4:186071/1‑149 (MQ=255)
                                                                                                                                  tCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGCTCAGGAAAACCTGGTGCGt                <  2:89803/149‑1 (MQ=255)
                                                                                                                                       cAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGCTCAGGAAAACCTGGTGCGTATCt            <  4:352413/148‑1 (MQ=255)
                                                                                                                                       cAGCGCCTGCGTTGCCGGCCGGGCTACCGCGCGGGCCTGCGCGTCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCAAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGCTCAGGAAAACCTGGTGCGTATCt            <  3:11391/148‑1 (MQ=255)
                                                                                                                                       cAGAGCCTGCTTGGCCGGACGGTCTACCGCTCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCTGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGCTCAGGAAAACCTGGTGCGTATCt            <  3:16981/148‑1 (MQ=255)
                                                                                                                                         gcgcCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGCTCAGGAAAACCTGGTGCGTATCtgtg         >  7:270735/1‑149 (MQ=255)
                                                                                                                                           gcCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGCTCAGGAAAACCTGGTGCGTATCTGTGcc       <  7:302034/149‑1 (MQ=255)
                                                                                                                                             cTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGCTCAGGAAAACCTGGTGCGTATCTGTGCCGa     <  4:124203/149‑1 (MQ=255)
                                                                                                                                                cTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGCTCAGGAAAACCTGGTGCGTATCTGTGCCCAGCa  >  5:129018/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATCTCCATGGCCGGTGCCGCCTCGGATGTACTGGCGGTGCAACTGTTGCTCAAGGAAGCCGGCCTGACCCGCCCCATGCGCGTGGTGCCACTGTTCGAGACCCTGGCCGACCTCGACAACGCCGGCCCGGTCATGCAGCGCCTGCTTGGCCTGCCGGGCTACCGCGCGGGCCTGCGCGGCCCGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAAGGACGCCGGCACCACCGCTGCTGCCTGGGCGCAGTACCGGGCTCAGGAAAACCTGGTGCGTATCTGTGCCGAGCA  >  NC_002947/1710789‑1711081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑