Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,714,464	C→A	6.2%	P103Q (CCG→CAG)	PP_1509 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,714,464	0	C	A	6.2%	248.8 / 4.0	80	P103Q (CCG→CAG)	PP_1509	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (36/39);  new base A (3/2);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

CCCGCTCCCGGCACCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTGCGCAACCAGGCCTTTTTTGCCAAGGGTGAG  >  NC_002947/1714319‑1714612                                                                                                                                                    |                                                                                                                                                     cccGCTCCCGGCACCATTGCCCGCTGGGCGCAGCTCATGCCCGATGCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGATTGAGCCTGCATTCGACTTCACCCGGCTGATGGCCCAGc                                                                                                                                                    >  1:84309/1‑149 (MQ=255)  ccGCTCCCGGCACCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTg                                                                                                                                                  >  2:122442/1‑149 (MQ=255)  ccGCTCCCGGCACCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTg                                                                                                                                                  <  5:416061/149‑1 (MQ=255)   cGCTCCCGGCACCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTgg                                                                                                                                                 <  7:66326/149‑1 (MQ=255)       cccGGCACCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCg                                                                                                                                             >  3:356774/1‑149 (MQ=255)        ccGGCACCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCAGCGGGGccat                                                                                                                                            >  3:152496/1‑147 (MQ=255)          ggCACCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGgcg                                                                                                                                          <  1:166463/149‑1 (MQ=255)          ggCACCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCAGCGGGGccatca                                                                                                                                          <  4:152496/149‑5 (MQ=255)           gCACCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGgcgc                                                                                                                                         <  4:356774/149‑1 (MQ=255)           gCACCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGgcgc                                                                                                                                         <  2:385190/149‑1 (MQ=255)           gCACCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCAGCGGGGccatcag                                                                                                                                         <  2:84309/149‑6 (MQ=255)             aCCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGcgcg                                                                                                                                        >  7:351979/1‑148 (MQ=255)             aCCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGcgcg                                                                                                                                        >  5:172188/1‑148 (MQ=255)             aCCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGt                                                                                                                                       <  1:122442/149‑1 (MQ=255)               cATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGTCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTAt                                                                                                                                     >  5:194173/1‑149 (MQ=255)                 ttGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCa                                                                                                                                   <  4:135821/149‑1 (MQ=255)                           ggCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGc                                                                                                                         <  6:172188/149‑1 (MQ=255)                             cgcAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTg                                                                                                                       >  1:357290/1‑149 (MQ=255)                                  cTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGtt                                                                                                                  <  2:258955/149‑1 (MQ=255)                                     aTGCCCGA‑GCACTTTCGCTTCACTTCCAAGTTTCCGCGGGATGTCAGCGATGAAGGCGACGTTCGAGACC‑AGCTTGTGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTTCAGTTGc                                                                                                                <  2:358320/148‑1 (MQ=255)                                      tGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCg                                                                                                              <  6:350432/149‑1 (MQ=255)                                       gCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCgg                                                                                                             >  1:183260/1‑149 (MQ=255)                                           gA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGcc                                                                                                           >  5:284638/1‑147 (MQ=255)                                               cACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGtt                                                                                                      <  1:159372/149‑1 (MQ=255)                                                aCTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTc                                                                                                     <  2:148021/149‑1 (MQ=255)                                                 cTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCg                                                                                                    <  4:284859/149‑1 (MQ=255)                                                  tttCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCgg                                                                                                   >  1:196620/1‑149 (MQ=255)                                                  tttCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCgg                                                                                                   >  6:372029/1‑149 (MQ=255)                                                    tCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGTCTGATGGCCCCTCTGGGCCGGAGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGcc                                                                                                 >  4:216379/1‑149 (MQ=255)                                                    tCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGcc                                                                                                 >  1:104366/1‑149 (MQ=255)                                                     cGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGcc                                                                                                 <  6:284638/148‑1 (MQ=255)                                                       cTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCgg                                                                                              >  4:286872/1‑149 (MQ=255)                                                       cTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCgg                                                                                              >  5:59440/1‑149 (MQ=255)                                                             tGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGtt                                                                                        >  7:318319/1‑149 (MQ=255)                                                               ccAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTg                                                                                      >  6:323767/1‑149 (MQ=255)                                                               cataGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGttt                                                                                       <  4:272786/145‑1 (MQ=255)                                                                cAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGTCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTgg                                                                                     <  6:194173/149‑1 (MQ=255)                                                                cAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTgg                                                                                     <  5:372029/149‑1 (MQ=255)                                                                cAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTgg                                                                                     <  5:323767/149‑1 (MQ=255)                                                                cAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCAGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTgg                                                                                     >  5:418266/1‑149 (MQ=255)                                                                 aaGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCATTCGACTTCACCAGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTggg                                                                                    >  3:236520/1‑149 (MQ=255)                                                                      tCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGc                                                                               >  4:390428/1‑149 (MQ=255)                                                                      tCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGc                                                                               >  6:221452/1‑149 (MQ=255)                                                                        cgcgGGATGTCAGCCATGAAGGCGACCTGCGAGACCTAGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGcc                                                                                                           >  1:66457/1‑120 (MQ=255)                                                                        cgcgGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGcc                                                                                                           <  2:66457/119‑1 (MQ=255)                                                                         gcgGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCttt                                                                            >  3:398858/1‑149 (MQ=255)                                                                         gcgGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCttt                                                                            <  5:135578/149‑1 (MQ=255)                                                                         gcgGGATGGCAGCCATGAAGGCGACCTGCGAGACC‑AGATTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTTCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCttt                                                                            <  4:236520/149‑1 (MQ=255)                                                                          cgGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTg                                                                           >  5:175053/1‑149 (MQ=255)                                                                                      cATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGa                                                               <  2:104366/149‑1 (MQ=255)                                                                                         ggaGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGAt                                                              >  4:372171/3‑147 (MQ=255)                                                                                          aaGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCa                                                            <  8:318319/148‑1 (MQ=255)                                                                                           aGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGAt                                                              >  2:153114/1‑145 (MQ=255)                                                                                             gCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAgtat                                                        >  5:358160/1‑146 (MQ=255)                                                                                                aCCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGTCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCTTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGcc                                                     <  6:418266/149‑1 (MQ=255)                                                                                                aCCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGcc                                                     <  2:196620/149‑1 (MQ=255)                                                                                                aCCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCCGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGcc                                                     >  6:219637/1‑149 (MQ=255)                                                                                                  cTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGt                                                   <  1:352129/149‑1 (MQ=255)                                                                                                   tGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTc                                                                         >  4:279039/1‑126 (MQ=255)                                                                                                   tGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTc                                                                         <  3:279039/126‑1 (MQ=255)                                                                                                     cGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGc                                                 <  2:183260/148‑1 (MQ=255)                                                                                                       agaCC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCccg                                              <  6:59440/149‑1 (MQ=255)                                                                                                       agaCC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCccg                                              >  7:121058/1‑149 (MQ=255)                                                                                                         aCC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCccgtg                                            <  3:390428/149‑1 (MQ=255)                                                                                                          cc‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCccgtg                                            <  5:221452/148‑1 (MQ=255)                                                                                                           c‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCccgtggc                                          >  5:326936/1‑149 (MQ=255)                                                                                                              gCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCccgtggccg                                        >  3:105954/1‑149 (MQ=255)                                                                                                               cTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGTTCAGATTGCCGTGCccgtggccgt                                       <  7:57898/149‑1 (MQ=255)                                                                                                                  gAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGGTTGGGTGAGCTTTGTAACTTCATCGATCAGATTGCCGTGCcactggccgtgg                                     >  7:345399/1‑148 (MQ=255)                                                                                                                     cctgcctTCGACTTCACCCGGCTGATGGCCCCGCTGGGTCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGa                                                          >  5:150537/1‑124 (MQ=255)                                                                                                                     cctgcctTCGACTTCACCCGGCTGATGGCCCCGCTGGGTCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGa                                                          <  6:150537/124‑1 (MQ=255)                                                                                                                         cctTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTgc                               <  7:222501/147‑1 (MQ=255)                                                                                                                          ctTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTgcgc                             >  4:330392/1‑148 (MQ=255)                                                                                                                           ttCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTGCGCaa                           >  7:358170/1‑149 (MQ=255)                                                                                                                           ttCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTGCGCaa                           >  7:27153/1‑149 (MQ=255)                                                                                                                               aCTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTGCGCAACCAg                       <  3:216379/149‑1 (MQ=255)                                                                                                                                 ttCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTGCGCaa                           <  7:21329/143‑1 (MQ=255)                                                                                                                                 ttCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTGCGCaa                           >  8:21329/1‑143 (MQ=255)                                                                                                                                                gCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTGCGCAACCAGGCCTTTTTTGCCAAgg       <  3:372171/148‑1 (MQ=255)                                                                                                                                                gCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTGCGCAACCAGGCCTTTTTTGCCAAgg       <  3:330392/148‑1 (MQ=255)                                                                                                                                                 ccccGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTGCGCAACCAGGCCTTTTTTGCCAAGGGt     >  7:127820/1‑149 (MQ=255)                                                                                                                                                 ccccGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTGCGCAACCAGGCCTTTTTTGCCAAGGGt     <  6:326936/149‑1 (MQ=255)                                                                                                                                                   ccGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTGCGCAACCAGGCCTTTTTTGCCAAGGGTGa   <  1:153114/149‑1 (MQ=255)                                                                                                                                                    cGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTGCGCAACCAGGCCTTTTTTGCCAAGGGTGAg  <  4:398858/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CCCGCTCCCGGCACCATTGCCCGCTGGGCGCAGCTCATGCCCGA‑GCACTTTCGCTTCACTGCCAAGTTTCCGCGGGATGTCAGCCATGAAGGCGACCTGCGAGACC‑AGCTTGAGCCTGCCTTCGACTTCACCCGGCTGATGGCCCCGCTGGGCCGGCGCGTATCACCCTACTGGCTGCAGTTGCCGGCCCAGTTCGGCCCGGCGCGTTTGGGTGAGCTTTGTCACTTCCTCGATCAGATTGCCGTGCCCGTGGCCGTGGAAGTGCGCAACCAGGCCTTTTTTGCCAAGGGTGAG  >  NC_002947/1714319‑1714612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑