Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,835,165 A→G 7.2% F132L (TTT→CTT)  PP_1639 ← SprT family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,835,1650AG 7.2% 156.2 / 2.3 55F132L (TTT→CTT) PP_1639SprT family protein
Reads supporting (aligned to +/- strand):  ref base A (26/25);  new base G (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACACCCCGCGCATGATCAGCTGCCACTCTTCACCATGGGCCTGGATGCG  >  NC_002947/1835023‑1835312
                                                                                                                                               |                                                                                                                                                   
cgctGGGCAGCGCACGTCTCGTGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATAGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGa                                                                                                                                                <  5:311952/148‑1 (MQ=255)
cgcgGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGaa                                                                                                                                               <  1:349346/149‑1 (MQ=255)
 gcgGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAg                                                                                                                                              >  8:237207/1‑149 (MQ=255)
  cgGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCACGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGt                                                                                                                                             <  2:82861/149‑1 (MQ=255)
   gggcCAGCGCACGACACGGGAATAGTACGACCCCCGATCAATTCGACCCGCGTCTCGCCTAAGTACACCAGGACTTACCGGCACC‑GCTTGCAAAGGAATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCCCGGTAAACGGGAAGTc                                                                                                                                            >  8:243175/1‑149 (MQ=255)
     ggCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGt                                                                                                                                             <  4:347742/146‑1 (MQ=255)
     ggCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGt                                                                                                                                             >  3:347742/1‑146 (MQ=255)
         gcgcACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTg                                                                                                                                      >  5:403082/1‑149 (MQ=255)
         gcgcACGTCTCGGGAATTGTACGACCCCCGATCTAATCGACCCGCGTCTCGCCGCTGTACCCCAGGACTTCCCGGCACC‑GCTTGAACAGGTAACGCCCCCCTTGGTGCACCAGCTTGAGCCGCTGCGCGGGAAGCGGGAAGTCGCTTTg                                                                                                                                      >  6:220936/1‑149 (MQ=255)
         gcgcACGTCTAGGGAATTGTACGACCCCCGATCTATTCGAACCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAGCGGGAAGTCGGTTTg                                                                                                                                      >  6:215396/1‑149 (MQ=255)
          cgcACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGc                                                                                                                                     <  6:403082/149‑1 (MQ=255)
          cgcACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGc                                                                                                                                     <  7:237207/149‑1 (MQ=255)
                tctcGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGcgggcac                                                                                                                               >  1:241993/1‑149 (MQ=255)
                tctcGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGcgggcac                                                                                                                               >  3:98227/1‑149 (MQ=255)
                     ggAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGTGAAGTCGCTTTGcgggcacggg                                                                                                                            >  7:119500/1‑147 (MQ=255)
                            tACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTa                                                                                                                    <  4:104357/148‑1 (MQ=255)
                             aCGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCgg                                                                                                                      >  8:156518/1‑145 (MQ=255)
                              cGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGAt                                                                                                                 <  4:98227/149‑1 (MQ=255)
                                   cccGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGc                                                                                                            >  8:191708/1‑149 (MQ=255)
                                   cccGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGc                                                                                                            <  1:147379/149‑1 (MQ=255)
                                                aaccGCGTCTCGCCGATGTACACCAGGACTTCCCTGCACC‑GCTTGCACAGGTAGCGCCGCCCTTTGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGt                                                                                                                     <  7:346689/125‑1 (MQ=255)
                                                  ccGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAGGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGcc                                                                                             >  7:220366/1‑149 (MQ=255)
                                                  ccGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGcc                                                                                             >  4:230859/1‑149 (MQ=255)
                                                        ctcGCCGCTGTACACCAGGACTTCCCGGCACA‑GCTTGCACACGTTTAGGCGCCCTTGGTGCACCAGCTTGTGCCGCTTCGCGGTAAACGGGAATGCGGTTTGCGGGCCCGGGCAGCGGGATAAGTAGCGGGTCACTTCGCGCCGTTGCa                                                                                       >  1:249288/1‑149 (MQ=255)
                                                           gccgcTGTACACCAGGACTTCCCGTCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTgcgc                                                                                                <  2:30338/137‑1 (MQ=255)
                                                           gccgcTGTACACCAGGACTTCCCGGCACCTGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTgcgc                                                                                                >  1:30338/1‑138 (MQ=255)
                                                           gccgcTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCAc                                                                                      <  5:400595/147‑1 (MQ=255)
                                                           gccgcTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCAc                                                                                      <  2:30132/147‑1 (MQ=255)
                                                             cgcTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCa                                                                                  <  8:283581/149‑1 (MQ=255)
                                                               cTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGACGTCGCTGTGCGGGCACGGGCAGCg                                                                                                                       >  7:168443/1‑110 (MQ=255)
                                                                    cacCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTtgt                                                                           <  7:156518/149‑1 (MQ=255)
                                                                     acgaGGACTTCCCGGCACC‑GCTTGCACAGGTATCGGCGCCCTTGGTGCACCAGCTTGTGACGCTGCGCGGTAAACGGTAAGTCGCTTTGCGGGCAGGGGCATCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTtgtg                                                                          <  7:243175/146‑1 (MQ=255)
                                                                                ccGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTg                                                                <  2:241993/148‑1 (MQ=255)
                                                                                      cc‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCa                                                         <  4:161377/149‑1 (MQ=255)
                                                                                       c‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGAAGCGGTAGATGTAGAGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAg                                                        >  8:369738/1‑149 (MQ=255)
                                                                                             gCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAGCTGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCa                                                    <  5:220936/148‑1 (MQ=255)
                                                                                             gCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAGCGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCa                                                    <  5:215396/148‑1 (MQ=255)
                                                                                              cacaGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATa                                                  <  3:230859/149‑1 (MQ=255)
                                                                                                 aGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATacac                                               >  8:202888/1‑149 (MQ=255)
                                                                                                  ggTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACAcc                                              <  7:191708/149‑1 (MQ=255)
                                                                                                        gccgccCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTg                                                                >  8:353248/1‑125 (MQ=255)
                                                                                                        gccgccCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTg                                                                <  7:353248/125‑1 (MQ=255)
                                                                                                        gccgccCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACACCCCgcgc                                        <  8:220366/149‑1 (MQ=255)
                                                                                                            cccTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACACACCGCGCATGa                                    >  6:33866/1‑149 (MQ=255)
                                                                                                              cTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACACCCCGCGCATGAt                                   >  4:71025/1‑148 (MQ=255)
                                                                                                              cTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACACCCCGCGCACGAt                                   >  2:373581/1‑148 (MQ=255)
                                                                                                                   tGCACCAGCTTGTGCGGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGCGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGGTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACACCCCGCGCATGATCAGCTg                             <  5:332329/149‑1 (MQ=255)
                                                                                                                      aCCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACACCCCGCGCATGATCAGCTGCCa                          >  3:9349/1‑149 (MQ=255)
                                                                                                                        cAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACACCCCGCGCATGATCAGCTGCCAc                         >  2:75760/1‑148 (MQ=255)
                                                                                                                        cAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGAGCCGTTGCACGTCATACTTGTGGCAACGATTGGGCGGCAGTTCATACACCCCGCGCATGATCAGCTGCCAct                        >  3:318870/1‑149 (MQ=255)
                                                                                                                          gCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACACCCCGCGCATGATCAGCTGCCActct                      >  5:328929/1‑149 (MQ=255)
                                                                                                                                   gCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACACCCCGCGCATGATCAGCTGCCACTCTTCACCATgg             <  6:328929/149‑1 (MQ=255)
                                                                                                                                   gCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACACCCCGCGCATGATCAGCTGCCACTCTTCACCATgg             <  6:150663/149‑1 (MQ=255)
                                                                                                                                     tGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACACCCCGCGCATGATCAGCTGCCACTCTTCACCATGGGc           >  5:61146/1‑149 (MQ=255)
                                                                                                                                            tAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACACCCCGCGCATGATCAGCTGCCACTCTTCACCATGGGCCTGGATg    >  8:312939/1‑149 (MQ=255)
                                                                                                                                              aaCGGGAAGTCGCTTTGAGGGCACGGGCATCGGTATATGTAGCGGGTCACTTCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGTCGGCAGTTCATACACCCCGCTCATGATCAGCTGCCACTCTTCACCATGGGCCTGGATGCg  <  5:33866/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
CGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACC‑GCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTGGCAACGATTGGGCGGCAGTTCATACACCCCGCGCATGATCAGCTGCCACTCTTCACCATGGGCCTGGATGCG  >  NC_002947/1835023‑1835312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑