Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,897,203 G→T 9.7% intergenic (‑56/+88) PP_mr24 ← / ← rdgC mini‑ykkC regulatory region/recombination‑associated protein RdgC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,897,2030GT 9.7% 183.4 / 8.9 62intergenic (‑56/+88)PP_mr24/rdgCmini‑ykkC regulatory region/recombination‑associated protein RdgC
Reads supporting (aligned to +/- strand):  ref base G (32/24);  new base T (2/4);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-01

GATGATCCAGGACATGAGTGTGTCTCCATCGTTTGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCACCACCCAGCGCCTCGAACAGCACCGGCAGGAAT  >  NC_002947/1897059‑1897348
                                                                                                                                                |                                                                                                                                                 
gatgatCCAGGACATGAGTGTGTCTCCATCGTTTGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACAATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGt                                                                                                                                                >  1:541/1‑148 (MQ=255)
     tCCAGGACATGAGTGTGTCTCCATCGTTTGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCAc                                                                                                                                          >  5:180874/1‑149 (MQ=255)
     tCCAGGACATGAGTGTGTCTCCATCGTTTGAATGATGGGGCCGACCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCAc                                                                                                                                          >  5:137475/1‑149 (MQ=255)
         ggACATGAGTGTGTCTCCATCGTTTGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGc                                                                                                                                      <  3:402782/149‑1 (MQ=255)
         ggACATGAGTGTGTCTCCATCGTTTGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGc                                                                                                                                      <  7:308819/149‑1 (MQ=255)
          gACATGAGGGTGTCTCCATCGTTTGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCa                                                                                                                                     <  1:263282/149‑1 (MQ=255)
           aCATGAGTGTGTCTCCATCGTTTGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTCGCGGGTAAACCCGCGCCTGCAAGTGGTAACCCCTTGCAg                                                                                                                                    <  3:190846/149‑1 (MQ=38)
               gAGTGTGTCTCCATCGTTTGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGgcgc                                                                                                                                >  7:88083/1‑149 (MQ=255)
                aGTGTGTCTCCATCGTTTGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGgcgc                                                                                                                                <  5:406802/148‑1 (MQ=255)
                aGTGTGTCTCCATCGTTTGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGcgcg                                                                                                                               <  5:169839/149‑1 (MQ=255)
                      tctcCATCGTTTGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAggg                                                                                                                                                  <  6:4409/124‑1 (MQ=255)
                      tctcCATCGTTTGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAggg                                                                                                                                                  >  5:4409/1‑124 (MQ=255)
                         ccATCGTTTGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTAccc                                                                                                                      >  1:31976/1‑149 (MQ=255)
                               tttGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGcgcg                                                                                                                               >  4:283810/1‑134 (MQ=255)
                               tttGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGcgcg                                                                                                                               <  3:283810/134‑1 (MQ=255)
                               tttGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTCGCGGGTAAACCCGCGCCTGCAAGTGGTAACCCCTTGCAGGCGCGGTCTTGCCcgcaagg                                                                                                                <  3:288341/149‑5 (MQ=21)
                                     tgatgGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCg                                                                                                          >  2:247914/1‑149 (MQ=255)
                                        tgGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTGTCCCGCGAAGAGGCCGGTg                                                                                                       >  4:268488/1‑149 (MQ=255)
                                        tgGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCAACATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTg                                                                                                       >  8:385664/1‑149 (MQ=255)
                                              cGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCa                                                                                                 <  8:307704/149‑1 (MQ=255)
                                                tCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATCGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGAAGGCGCGGGTTTACCAGCGAAGAGGCCGGTGCTCGCAg                                                                                                >  3:218942/1‑148 (MQ=255)
                                                           gTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAg                                                                                    >  5:331317/1‑149 (MQ=255)
                                                                  ggTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCa                                                                             >  7:175542/1‑149 (MQ=255)
                                                                        cggtGAGGACGACCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTgg                                                                                                   <  4:349862/119‑1 (MQ=255)
                                                                             aGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCa                                                                  <  6:331317/149‑1 (MQ=255)
                                                                              ggTCGTCCTCACCGTGGGCACCATGGTGCCTATCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCa                                                                  >  6:102553/1‑148 (MQ=255)
                                                                                 cgtcCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCa                                                                                     <  5:314870/126‑1 (MQ=255)
                                                                                 cgtcCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCa                                                                                     >  6:314870/1‑126 (MQ=255)
                                                                                     cTCACCGTGGGCACCATGGTGCCTATCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAAt                                                           <  5:102553/148‑1 (MQ=255)
                                                                                      tCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTAc                                                                                                                        >  2:46285/1‑86 (MQ=255)
                                                                                      tCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTAc                                                                                                                        <  1:46285/86‑1 (MQ=255)
                                                                                      tCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAAt                                                           >  2:177734/1‑147 (MQ=255)
                                                                                       cACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAAt                                                           >  5:369585/1‑146 (MQ=255)
                                                                                         ccGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGAc                                                      >  3:92997/1‑149 (MQ=255)
                                                                                            tGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGcc                                                                                 >  4:290044/1‑119 (MQ=255)
                                                                                            tGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGcc                                                                                 <  3:290044/119‑1 (MQ=255)
                                                                                               gCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTg                                                >  4:16680/1‑149 (MQ=255)
                                                                                                cACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCt                                                                                                     >  7:106163/1‑95 (MQ=255)
                                                                                                cACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCt                                                                                                     <  8:106163/95‑1 (MQ=255)
                                                                                                   cATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCa                                                                                <  1:349608/113‑1 (MQ=255)
                                                                                                   cATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCa                                                                                >  2:349608/1‑113 (MQ=255)
                                                                                                       gTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAAtc                                         <  2:31976/148‑1 (MQ=255)
                                                                                                           cTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTc                                    <  1:177734/149‑1 (MQ=255)
                                                                                                              tCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTca                                   <  3:16680/147‑1 (MQ=255)
                                                                                                                 gCGGTCTTGCCCGCAAGGCCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCaccacc                              >  5:232776/1‑149 (MQ=255)
                                                                                                                  cGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCaccacc                              >  2:16524/1‑148 (MQ=255)
                                                                                                                      ttcgCGGGTAAACCCGCGCCTGCAAGTGGTAACCCCTTGCAGGCGCGGTCTTGCCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCaccacc                              >  2:332009/4‑144 (MQ=25)
                                                                                                                      ttcgCGGGTAAACCCGCGCCTGCAAGTGGTAACCCCTTGCAGGCGCGGTCTTGCCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCaccacc                              <  1:332009/141‑1 (MQ=25)
                                                                                                                           gggAAAGACGGCGCCTGAAAGGGGTTACTACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCTGAATCTCTTCACCACCCATCGCCTc                     <  4:218942/148‑1 (MQ=255)
                                                                                                                             gCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCACCACCCAGCGCCTCGaa                  >  1:292204/1‑149 (MQ=255)
                                                                                                                              cAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCACCACCCAGCGCCTCGAAc                 <  8:175542/149‑1 (MQ=255)
                                                                                                                               aaGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGa                                                                                         <  5:1402/76‑1 (MQ=255)
                                                                                                                               aaGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGa                                                                                         >  6:1402/1‑76 (MQ=255)
                                                                                                                                   ccGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCACCACCCAGCGCCTCGAACAGCAc            <  8:88083/149‑1 (MQ=255)
                                                                                                                                     gcgcCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCACCACCCAGCGCCTCGAACAGCACCg          >  6:413151/1‑149 (MQ=255)
                                                                                                                                        ccTGCAAGTGGTAACCCCTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGc                                               <  6:332801/109‑1 (MQ=255)
                                                                                                                                        ccTGCAAGTGGTAACCCCTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGc                                               >  5:332801/1‑109 (MQ=255)
                                                                                                                                        ccTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCACCACCCAGCGCCTCGAACAGCACCGGCa       <  4:92997/149‑1 (MQ=255)
                                                                                                                                         cTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCACCACCCAGCGCCTCGAACAGCACCGGCAg      <  7:385664/149‑1 (MQ=255)
                                                                                                                                          tGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCACCACCCAGCGCCTCGAACAGCACCGGCAg      >  7:257726/1‑148 (MQ=255)
                                                                                                                                           gCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCACCACCCAGCGCCTCGAACAGCACCGGCAgg     >  1:365965/1‑148 (MQ=255)
                                                                                                                                            cAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCACCACCCAGCGCCTCGAACAGCACCGGCAGGaa   >  3:349410/1‑149 (MQ=255)
                                                                                                                                             aaGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCACCACCCAGCGCCTCGAACAGCACCGGCAGGAAt  >  2:387717/1‑149 (MQ=255)
                                                                                                                                             aaGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCACCACCCAGCGCCTCGAACAGCACCGGCAGGAAt  >  3:228183/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GATGATCCAGGACATGAGTGTGTCTCCATCGTTTGAATGATGGGGCCGTCCCCGGAGTGGTCACGCGGTGCGTCGTGAGGTCGTCCTCACCGTGGGCACCATGGTGCCTGTCTGCGGCCTTGCGGGCAAGACCGCGCCTGCAAGGGGTTACCACTTGCAGGCGCGGGTTTACCCGCGAAGAGGCCGGTGCTGGCAGAAAACGATTCAGGCCAGCACCTGCATTTCATACCAATCAGACCCCTTGCGGAATCTCTTCACCACCCAGCGCCTCGAACAGCACCGGCAGGAAT  >  NC_002947/1897059‑1897348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑