Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,918,256 C→G 10.9% intergenic (‑32/+86) PP_1718 ← / ← prc EAL domain‑containing diguanylate phosphodiesterase/tail‑specific protease Prc

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,918,2560CG10.9% 153.8 / 10.7 55intergenic (‑32/+86)PP_1718/prcEAL domain‑containing diguanylate phosphodiesterase/tail‑specific protease Prc
Reads supporting (aligned to +/- strand):  ref base C (22/27);  new base G (2/4);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01

GCCTCATGGCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGCGACCGGTTTCCG  >  NC_002947/1918109‑1918403
                                                                                                                                                   |                                                                                                                                                   
gcCTCATGGCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCt                                                                                                                                                    <  6:207598/149‑1 (MQ=255)
  gccgtGGCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGc                                                                                                                                                  <  2:168244/145‑1 (MQ=255)
     gtGGCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGACGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATg                                                                                                                                               <  6:166130/148‑1 (MQ=255)
      tGGCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGc                                                                                                                                              <  3:293594/149‑1 (MQ=255)
        gCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGccc                                                                                                                                            <  5:10859/149‑1 (MQ=255)
         ccGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCg                                                                                                                                           >  8:331313/1‑149 (MQ=255)
         ccGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCg                                                                                                                                           <  4:333925/149‑1 (MQ=255)
          cGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATGCAGATGCCCgg                                                                                                                                          <  8:350016/149‑1 (MQ=255)
           gAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATGCAGATGCCCGGc                                                                                                                                         <  4:9246/149‑1 (MQ=255)
            taTAGCCTAGGTTCAGTCAGGCACATGATTGGCTGGAACAGACTGTGAATAGTGCGCTGAGCCAGGATGGCACTCAAGGGACTGAGCTGTTCGGCAACTGTGATGATGGATTCCTGAAAAGTAATGGCCGGGCATGCAGATGCCCGGcc                                                                                                                                        <  6:130901/148‑1 (MQ=255)
                 ccTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTCCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCa                                                                                                                                    >  5:134669/1‑148 (MQ=255)
                  cTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCata                                                                                                                                  <  2:10019/149‑1 (MQ=255)
                       ttCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCa                                                                                                                             >  7:339766/1‑149 (MQ=255)
                       ttCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCa                                                                                                                             <  3:119817/149‑1 (MQ=255)
                              aGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACa                                                                                                                        <  3:215547/147‑1 (MQ=255)
                                  acaTGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGa                                                                                                                  >  1:62905/1‑149 (MQ=255)
                                  acaTGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGa                                                                                                                  >  3:114750/1‑149 (MQ=255)
                                           gCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGttt                                                                                                         >  2:147446/1‑149 (MQ=255)
                                            cTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGttt                                                                                                         >  8:148494/1‑148 (MQ=255)
                                            cTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTtg                                                                                                        <  1:238831/149‑1 (MQ=255)
                                             tGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTtga                                                                                                       >  3:310612/1‑149 (MQ=255)
                                              ggAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTtgat                                                                                                      >  6:7193/1‑149 (MQ=255)
                                               gAAGAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGaa                                                                                                                 >  2:344897/1‑136 (MQ=255)
                                               gAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTtgat                                                                                                      <  7:331313/148‑1 (MQ=255)
                                                         tgAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATcaca                                                                                           <  5:262110/149‑1 (MQ=255)
                                                         tgAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATcaca                                                                                           <  5:256269/149‑1 (MQ=255)
                                                            aTACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACatt                                                                                         <  2:62905/148‑1 (MQ=255)
                                                                tGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCat                                                                                                                                   <  2:110650/102‑1 (MQ=255)
                                                                tGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCat                                                                                                                                   >  1:110650/1‑102 (MQ=255)
                                                                  cgcTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGc                                                                                  >  5:13065/1‑149 (MQ=255)
                                                                   gcTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGcc                                                                                 <  5:7193/149‑1 (MQ=255)
                                                                       aGCCAGGATGGCACTCAAGGCTCTGAGGTGTTCGGCAACGGTCATGATGGATTCCTGAAAATTCAGGTCCGGGCATCTGCATGCCCGGCCCTCATTTTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCcatc                                                                              <  2:142662/148‑1 (MQ=255)
                                                                        gCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCcatcat                                                                            >  1:75681/1‑149 (MQ=255)
                                                                               tGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAg                                                                     <  1:379305/149‑1 (MQ=255)
                                                                                ggCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAgg                                                                    >  4:152429/1‑149 (MQ=255)
                                                                                    cTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGcc                                                                <  4:310612/149‑1 (MQ=255)
                                                                                     tCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCt                                                               <  1:147446/149‑1 (MQ=255)
                                                                                       aaGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCt                                                               <  3:152429/147‑1 (MQ=255)
                                                                                        aGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATAATTCTTCCATCATTGCAGAGGTGCCTTa                                                             >  1:137299/1‑148 (MQ=255)
                                                                                                aGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTg                                                      <  6:134669/147‑1 (MQ=255)
                                                                                                  cTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATGCAGATGCCCGGcc                                                                                                                                        >  4:205460/1‑63 (MQ=25)
                                                                                                  cTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATGCAGATGCCCGGcc                                                                                                                                        <  3:205460/63‑1 (MQ=25)
                                                                                                   tGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCAc                                                 >  2:46938/1‑149 (MQ=255)
                                                                                                         gCATCGGTCATGAGCGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTAGTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGt                                           <  8:339766/149‑1 (MQ=255)
                                                                                                           aaCGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGc                                         >  5:133847/1‑149 (MQ=255)
                                                                                                              ggTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTgg                                      <  7:148494/149‑1 (MQ=255)
                                                                                                                tCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCa                                    >  4:168128/1‑149 (MQ=255)
                                                                                                                        gATTCCTGTAAAGTAATGGACGGGCATCTGCATGCCCGGCCATCATATTTCACGACAGCACGATGACTGTTTGATTACAGATCACATTATTCTGACATCATTGATGAGGTTCCTTACTTCTTGTCCACCTTTGTGCTGGCACTGAGGTa                            <  6:13065/149‑1 (MQ=255)
                                                                                                                               gAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGt                                           <  2:303641/127‑1 (MQ=255)
                                                                                                                               gAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGt                                           >  1:303641/1‑127 (MQ=255)
                                                                                                                                    gTATGGGCCGGGCATCTTCATGCCCGGCCATCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCTCATTATTCTGCCATAATTGCTGAGTTGCCTTACTTCTTGGCAACCTTTGTTCTGGCACTGAGGTAGTCGATCAGGAt                <  2:137299/149‑1 (MQ=255)
                                                                                                                                      gaGGGCCGGGCATGCAGATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGc              >  8:27252/2‑149 (MQ=255)
                                                                                                                                             gggCATCTGCATGCCCGGCCATCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTATTGGCCACCTTGGTGCTCGCACTGAGGTAGTCGATCAGGATGCGACCGGt       >  8:68963/1‑149 (MQ=255)
                                                                                                                                               gCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGCGACCGGttt     >  2:364983/1‑149 (MQ=255)
                                                                                                                                                  tCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGAGACCGGTTTcct  >  6:26507/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCCTCATGGCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGCGACCGGTTTCCG  >  NC_002947/1918109‑1918403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑