Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,918,259 C→G 11.3% intergenic (‑35/+83) PP_1718 ← / ← prc EAL domain‑containing diguanylate phosphodiesterase/tail‑specific protease Prc

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,918,2590CG11.3% 140.8 / 10.8 53intergenic (‑35/+83)PP_1718/prcEAL domain‑containing diguanylate phosphodiesterase/tail‑specific protease Prc
Reads supporting (aligned to +/- strand):  ref base C (22/25);  new base G (2/4);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-02

CTCATGGCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGCGACCGGTTTCCGCGAG  >  NC_002947/1918111‑1918407
                                                                                                                                                    |                                                                                                                                                    
gccgtGGCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGc                                                                                                                                                      <  2:168244/145‑1 (MQ=255)
   gtGGCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGACGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATg                                                                                                                                                   <  6:166130/148‑1 (MQ=255)
    tGGCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGc                                                                                                                                                  <  3:293594/149‑1 (MQ=255)
      gCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGccc                                                                                                                                                <  5:10859/149‑1 (MQ=255)
       ccGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCg                                                                                                                                               <  4:333925/149‑1 (MQ=255)
       ccGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCg                                                                                                                                               >  8:331313/1‑149 (MQ=255)
        cGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATGCAGATGCCCgg                                                                                                                                              <  8:350016/149‑1 (MQ=255)
         gAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATGCAGATGCCCGGc                                                                                                                                             <  4:9246/149‑1 (MQ=255)
          taTAGCCTAGGTTCAGTCAGGCACATGATTGGCTGGAACAGACTGTGAATAGTGCGCTGAGCCAGGATGGCACTCAAGGGACTGAGCTGTTCGGCAACTGTGATGATGGATTCCTGAAAAGTAATGGCCGGGCATGCAGATGCCCGGcc                                                                                                                                            <  6:130901/148‑1 (MQ=255)
               ccTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTCCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCa                                                                                                                                        >  5:134669/1‑148 (MQ=255)
                cTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCata                                                                                                                                      <  2:10019/149‑1 (MQ=255)
                     ttCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCa                                                                                                                                 >  7:339766/1‑149 (MQ=255)
                     ttCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCa                                                                                                                                 <  3:119817/149‑1 (MQ=255)
                            aGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACa                                                                                                                            <  3:215547/147‑1 (MQ=255)
                                acaTGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGa                                                                                                                      >  1:62905/1‑149 (MQ=255)
                                acaTGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGa                                                                                                                      >  3:114750/1‑149 (MQ=255)
                                         gCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGttt                                                                                                             >  2:147446/1‑149 (MQ=255)
                                          cTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGttt                                                                                                             >  8:148494/1‑148 (MQ=255)
                                          cTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTtg                                                                                                            <  1:238831/149‑1 (MQ=255)
                                           tGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTtga                                                                                                           >  3:310612/1‑149 (MQ=255)
                                            ggAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTtgat                                                                                                          >  6:7193/1‑149 (MQ=255)
                                             gAAGAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGaa                                                                                                                     >  2:344897/1‑136 (MQ=255)
                                             gAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTtgat                                                                                                          <  7:331313/148‑1 (MQ=255)
                                                       tgAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATcaca                                                                                               <  5:262110/149‑1 (MQ=255)
                                                       tgAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATcaca                                                                                               <  5:256269/149‑1 (MQ=255)
                                                          aTACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACatt                                                                                             <  2:62905/148‑1 (MQ=255)
                                                              tGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCat                                                                                                                                       >  1:110650/1‑102 (MQ=255)
                                                              tGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCat                                                                                                                                       <  2:110650/102‑1 (MQ=255)
                                                                cgcTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGc                                                                                      >  5:13065/1‑149 (MQ=255)
                                                                 gcTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGcc                                                                                     <  5:7193/149‑1 (MQ=255)
                                                                     aGCCAGGATGGCACTCAAGGCTCTGAGGTGTTCGGCAACGGTCATGATGGATTCCTGAAAATTCAGGTCCGGGCATCTGCATGCCCGGCCCTCATTTTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCcatc                                                                                  <  2:142662/148‑1 (MQ=255)
                                                                      gCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCcatcat                                                                                >  1:75681/1‑149 (MQ=255)
                                                                             tGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAg                                                                         <  1:379305/149‑1 (MQ=255)
                                                                              ggCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAgg                                                                        >  4:152429/1‑149 (MQ=255)
                                                                                  cTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGcc                                                                    <  4:310612/149‑1 (MQ=255)
                                                                                   tCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCt                                                                   <  1:147446/149‑1 (MQ=255)
                                                                                     aaGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCt                                                                   <  3:152429/147‑1 (MQ=255)
                                                                                      aGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATAATTCTTCCATCATTGCAGAGGTGCCTTa                                                                 >  1:137299/1‑148 (MQ=255)
                                                                                              aGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTg                                                          <  6:134669/147‑1 (MQ=255)
                                                                                                cTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATGCAGATGCCCGGcc                                                                                                                                            <  3:205460/63‑1 (MQ=25)
                                                                                                cTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATGCAGATGCCCGGcc                                                                                                                                            >  4:205460/1‑63 (MQ=25)
                                                                                                 tGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCAc                                                     >  2:46938/1‑149 (MQ=255)
                                                                                                       gCATCGGTCATGAGCGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTAGTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGt                                               <  8:339766/149‑1 (MQ=255)
                                                                                                         aaCGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGc                                             >  5:133847/1‑149 (MQ=255)
                                                                                                            ggTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTgg                                          <  7:148494/149‑1 (MQ=255)
                                                                                                              tCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCa                                        >  4:168128/1‑149 (MQ=255)
                                                                                                                      gATTCCTGTAAAGTAATGGACGGGCATCTGCATGCCCGGCCATCATATTTCACGACAGCACGATGACTGTTTGATTACAGATCACATTATTCTGACATCATTGATGAGGTTCCTTACTTCTTGTCCACCTTTGTGCTGGCACTGAGGTa                                <  6:13065/149‑1 (MQ=255)
                                                                                                                             gAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGt                                               <  2:303641/127‑1 (MQ=255)
                                                                                                                             gAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGt                                               >  1:303641/1‑127 (MQ=255)
                                                                                                                                  gTATGGGCCGGGCATCTTCATGCCCGGCCATCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCTCATTATTCTGCCATAATTGCTGAGTTGCCTTACTTCTTGGCAACCTTTGTTCTGGCACTGAGGTAGTCGATCAGGAt                    <  2:137299/149‑1 (MQ=255)
                                                                                                                                    gaGGGCCGGGCATGCAGATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGc                  >  8:27252/2‑149 (MQ=255)
                                                                                                                                           gggCATCTGCATGCCCGGCCATCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTATTGGCCACCTTGGTGCTCGCACTGAGGTAGTCGATCAGGATGCGACCGGt           >  8:68963/1‑149 (MQ=255)
                                                                                                                                             gCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGCGACCGGttt         >  2:364983/1‑149 (MQ=255)
                                                                                                                                                tCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGAGACCGGTTTcct      >  6:26507/1‑148 (MQ=255)
                                                                                                                                                    cATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGCGACCGGTTTCCGCGAg  >  7:117311/1‑149 (MQ=255)
                                                                                                                                                    cATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGCGACCGGTTTCCGCGAg  >  3:397530/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTCATGGCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGCGACCGGTTTCCGCGAG  >  NC_002947/1918111‑1918407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑