Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,023,489	A→G	5.3%	E174G (GAG→GGG)	rmd →	oxidoreductase Rmd

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,023,489	0	A	G	5.3%	225.7 / 2.2	75	E174G (GAG→GGG)	rmd	oxidoreductase Rmd
Reads supporting (aligned to +/- strand):  ref base A (37/34);  new base G (2/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

GGGCGAGCAGACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGCCCGCTGGGC  >  NC_002947/2023349‑2023637                                                                                                                                             |                                                                                                                                                      gggCGAGCAGACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCCTCACGCGGCCGTTCAATTACACCGGTGTTGGGCAGGCAGAGAAtttac                                                                                                                                               >  5:167145/1‑147 (MQ=255) gggCGAGCAGACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTcc                                                                                                                                               >  8:188992/1‑149 (MQ=255)          gACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGccc                                                                                                                                      >  1:42623/1‑148 (MQ=255)           aCCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCA‑GCAGGAAATTCTCTGCCTGCCCaa                                                                                                                                    <  5:187428/149‑1 (MQ=255)             cccGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAAttt                                                                                                                                                 <  7:178356/135‑1 (MQ=255)             cccGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAAttt                                                                                                                                                 >  8:178356/1‑135 (MQ=255)               cGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGAtt                                                                                                                                >  1:217339/1‑149 (MQ=255)                     cgcCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGGCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTc                                                                                                                           <  8:80640/148‑1 (MQ=255)                        cGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCAt                                                                                                                       >  1:367513/1‑149 (MQ=255)                          gCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCAttt                                                                                                                     >  4:189993/1‑149 (MQ=255)                            cAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTc                                                                                                                   >  7:272432/1‑149 (MQ=255)                                gATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTg                                                                                                                               <  1:17893/133‑1 (MQ=255)                                gATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTg                                                                                                                               >  2:17893/1‑133 (MQ=255)                                 aTTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTcgccgc                                                                                                              <  2:42623/149‑1 (MQ=255)                                   tACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTcgccgc                                                                                                              >  3:159405/1‑147 (MQ=255)                                    aCGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCaa                                                                                                            <  2:367513/148‑1 (MQ=255)                                      gcgGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGc                                                                                                         >  7:286890/1‑149 (MQ=255)                                        ggTCAGCAATTTGTCCTTGTAATACCTGGCGCGGCTTTTGTGGTACCGTTTTCCTTTCGTCTTCACGCTTCCTTTCTATTACACCGGTGTTGGGCAGGCAGAGATTTTCCTG‑CGGCGCAAGATTGTTTCGCATTTTCTCCTAAAGGCCg                                                                                                       <  6:167145/149‑1 (MQ=255)                                          tCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGa                                                                                                      >  7:83423/1‑148 (MQ=255)                                             gCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACAcc                                                                                                  <  4:350571/149‑1 (MQ=255)                                              cAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGcc                                                                                                        >  3:160070/1‑142 (MQ=255)                                              cAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGcc                                                                                                        <  4:160070/142‑1 (MQ=255)                                              cAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGaca                                                                                                    <  2:251603/146‑1 (MQ=255)                                              cAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGaca                                                                                                    >  1:251603/1‑146 (MQ=255)                                                 gTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCg                                                                                              <  8:281017/149‑1 (MQ=255)                                                  ttGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGa                                                                                             <  4:159405/149‑1 (MQ=255)                                                  cgcgCCATGTATTCCATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATc                                                                                               >  3:349806/4‑147 (MQ=255)                                                    ggCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAg                                                                                            <  4:75132/148‑1 (MQ=255)                                                      ccATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTc                                                                                         >  3:106821/1‑149 (MQ=255)                                                       cATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATc                                                                                               >  7:339850/1‑142 (MQ=255)                                                            attccATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCg                                                                                        >  3:245852/5‑144 (MQ=255)                                                            attccATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCg                                                                                        <  4:245852/140‑1 (MQ=255)                                                            aaTACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAAc                                                                                   <  8:50105/149‑1 (MQ=255)                                                                cATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTa                                                                                <  1:190398/148‑1 (MQ=255)                                                                 aTGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCCCGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGAAAGGCCGACACCATCGAGCTCGGCAACCTAGa                                                                              >  6:287532/1‑149 (MQ=255)                                                                 aTGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGa                                                                              >  5:169480/1‑149 (MQ=255)                                                                 aTGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCCCAAGGCCGACACCATCGAGCTCGGCAACCTAGa                                                                              >  7:352459/1‑149 (MQ=255)                                                                  tGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGAc                                                                             <  8:272432/149‑1 (MQ=255)                                                                  tGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGAc                                                                             >  2:305931/1‑149 (MQ=255)                                                                   ggCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGAc                                                                             <  4:349806/148‑1 (MQ=255)                                                                    gcgcGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACg                                                                            <  6:169480/148‑1 (MQ=255)                                                                     cgcgGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTa                                                                          <  2:217339/149‑1 (MQ=255)                                                                     cgcgGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTATCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTa                                                                          <  5:121661/149‑1 (MQ=255)                                                                      gcgGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTAt                                                                         <  5:241384/149‑1 (MQ=255)                                                                               gtgtGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATc                                                                                               <  5:20776/118‑1 (MQ=255)                                                                               gtgtGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATc                                                                                               >  6:20776/1‑118 (MQ=255)                                                                                 gtgACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGAttt                                                               >  5:363627/1‑148 (MQ=255)                                                                                       gTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGAt                                                        <  7:188992/149‑1 (MQ=255)                                                                                        tttGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGAAACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATg                                                       >  7:55860/1‑149 (MQ=255)                                                                                                tCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTg                                                 <  8:361226/147‑1 (MQ=255)                                                                                                tCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGc                                                <  8:83423/148‑1 (MQ=255)                                                                                                tCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGc                                                <  6:363627/148‑1 (MQ=255)                                                                                                tCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCg                                               >  6:44416/1‑149 (MQ=255)                                                                                                     atcaCGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCTACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGt                                          <  7:259844/149‑1 (MQ=255)                                                                                                     atcaCGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCg                                           <  1:305931/148‑1 (MQ=255)                                                                                                         cgcgTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAg                                      >  4:76978/1‑149 (MQ=255)                                                                                                          gcgTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAgg                                     >  4:231662/1‑149 (MQ=255)                                                                                                            gTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCg                                   >  8:147535/1‑149 (MQ=255)                                                                                                            gTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGAGATTTCAGCGATGTACGTGCGGTCGTGCAGGCg                                   >  4:384613/1‑149 (MQ=255)                                                                                                                     aTTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGc                          <  3:189993/149‑1 (MQ=255)                                                                                                                          acCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGAt                     >  3:116659/1‑149 (MQ=255)                                                                                                                            cGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTAc                                                    <  3:350816/116‑1 (MQ=255)                                                                                                                            cGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTAGGGCGCGATTTCAGCGATGTAc                                                    >  4:350816/1‑116 (MQ=255)                                                                                                                             ggTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATc                    >  7:97375/1‑147 (MQ=255)                                                                                                                              gtgtTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGaacccg                 >  8:358692/1‑144 (MQ=255)                                                                                                                              gtgtTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAg                 <  3:231662/149‑1 (MQ=255)                                                                                                                                 ttGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGGCGTGCAGGCGTACCGCGGCTTGATCGAGGCa              >  3:212220/1‑149 (MQ=255)                                                                                                                                 ttGGGCA‑GCAGGAAATTCTCTGCCTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACcg                              <  4:128469/133‑1 (MQ=255)                                                                                                                                 ttGGGCA‑GCAGGAAATTCTCTGCCTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACcg                              >  3:128469/1‑133 (MQ=255)                                                                                                                                 ttGGGCA‑GCAGGAAATTCTCTGCCTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCa              >  3:304659/1‑149 (MQ=255)                                                                                                                                   gggCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACg            >  8:205274/1‑149 (MQ=255)                                                                                                                                        ggcagAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGCCCGc       <  8:97375/149‑1 (MQ=255)                                                                                                                                          cagAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGc                                        >  1:176883/1‑114 (MQ=255)                                                                                                                                          cagAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGc                                        <  2:176883/114‑1 (MQ=255)                                                                                                                                          cagAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGCCCGCTg     <  4:106821/149‑1 (MQ=255)                                                                                                                                           agagAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGCCCGCTgg    <  3:76978/149‑1 (MQ=255)                                                                                                                                             agaATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGCCCGCTGGGc  <  5:44416/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                      GGGCGAGCAGACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGCCCGCTGGGC  >  NC_002947/2023349‑2023637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑