Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,052,784 G→T 8.2% intergenic (+16/+34) prmB → / ← PP_1828 N5‑glutamine S‑adenosyl‑L‑methionine‑dependent methyltransferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,052,7840GT 8.2% 153.0 / 3.8 49intergenic (+16/+34)prmB/PP_1828N5‑glutamine S‑adenosyl‑L‑methionine‑dependent methyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/22);  new base T (2/2);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

TCGAGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTACGCGCTC  >  NC_002947/2052644‑2052931
                                                                                                                                            |                                                                                                                                                   
tCGTGGCGTTTTTCCCCGTTGTTGACTTTTCCTGGCTGTTTTTCTTTCGGGGCGGTCATTGGGTTTTTATTCTTACCGCCGATTAGTGCCGCTAGTATCATGAATTGTTTAATGCCCTCGTTTAGTCTATTTGGGGCCGCGAAGCGGc                                                                                                                                              <  7:60634/148‑1 (MQ=255)
tCGAGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCTTCGCGGcc                                                                                                                                             >  3:221856/1‑149 (MQ=255)
tCGAGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGcc                                                                                                                                             <  1:297709/149‑1 (MQ=255)
tCGAGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCGCGTTTAGGCTATTTGGGGCCGCGAAGCGGc                                                                                                                                              <  6:341857/148‑1 (MQ=255)
   aGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCt                                                                                                                                           >  5:23815/1‑148 (MQ=255)
   aGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTa                                                                                                                                          <  4:378648/149‑1 (MQ=255)
       gTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCTTCGCGGCCCCaaata                                                                                                                                      <  4:221856/149‑4 (MQ=255)
       gTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGGTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGCAATTGTTCAAGGCCCTCGTTTAGGCTATTTAGGGCCGCTTCGCGGCCCCaaata                                                                                                                                      <  7:189695/149‑4 (MQ=255)
              cccGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCgg                                                                                                                               >  1:307986/1‑149 (MQ=255)
                    gTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATg                                                                                                                         >  5:271555/1‑149 (MQ=255)
                              ccTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGc                                                                                                               <  6:101089/149‑1 (MQ=255)
                               ctggctggAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCg                                                                                                              <  1:302887/149‑1 (MQ=255)
                                      gAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGc                                                                                                        >  2:289309/1‑148 (MQ=255)
                                       aGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCaa                                                                                                      <  2:307986/149‑1 (MQ=255)
                                        gTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAAt                                                                                                     <  3:33409/149‑1 (MQ=255)
                                         ttCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGGGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATc                                                                                                    <  1:289309/149‑1 (MQ=255)
                                            gAGCGGGGCGGGCGTGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATc                                                                                                    >  8:151119/1‑146 (MQ=255)
                                                 gggcgggcATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCaa                                                                                            <  3:268502/149‑1 (MQ=255)
                                                           ggggTGTTCATGCTCACCGCCGAGCAGTGCCGCCGGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCcctgcc                                                                                  >  5:173865/1‑149 (MQ=255)
                                                           ggggTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCcctgcc                                                                                  >  7:142469/1‑149 (MQ=255)
                                                            gggTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCcctgcct                                                                                 >  2:305836/1‑149 (MQ=255)
                                                             ggTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCcctgcctg                                                                                >  3:159320/1‑149 (MQ=255)
                                                              gtgtTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCcctgcctg                                                                                >  5:118169/1‑148 (MQ=255)
                                                                gtTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGaa                                                                             <  8:142469/149‑1 (MQ=255)
                                                                 ttCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGaa                                                                             <  1:305836/148‑1 (MQ=255)
                                                                   cATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAacac                                                                          >  3:32993/1‑149 (MQ=255)
                                                                    aTGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACg                                                                         <  5:180046/149‑1 (MQ=255)
                                                                            cgccgAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGc                                                                 <  6:271555/149‑1 (MQ=255)
                                                                            cgccgAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGc                                                                 <  6:23815/149‑1 (MQ=255)
                                                                                gAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGACCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGccac                                                              >  4:2893/1‑148 (MQ=255)
                                                                                     gTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACcagg                                                          >  6:103803/1‑147 (MQ=255)
                                                                                      tGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACcaggcag                                                       >  4:354988/1‑149 (MQ=255)
                                                                                            cAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATg                                                 >  7:315267/1‑149 (MQ=255)
                                                                                                  cAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACgg                                                                        <  2:116716/120‑1 (MQ=255)
                                                                                                   aGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGt                                               <  7:166157/144‑1 (MQ=255)
                                                                                                   aGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGt                                               >  8:166157/1‑144 (MQ=255)
                                                                                                    ggAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGTTGCGGTCTTAGCGGGTAGCAATCCAGAt                                                                                               <  1:227538/95‑1 (MQ=255)
                                                                                                    ggAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGAt                                                                                               >  2:227538/1‑95 (MQ=255)
                                                                                                       aTTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACTCCACCAGGAAGGTAATGGTGAACCGCa                                       >  6:303800/1‑148 (MQ=255)
                                                                                                            tCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATccgc                                 >  4:46677/1‑149 (MQ=255)
                                                                                                              aaGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATccgcc                                <  6:173865/148‑1 (MQ=255)
                                                                                                                    cTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTcg                         <  4:159320/149‑1 (MQ=255)
                                                                                                                       gTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCgcgc                      >  3:154314/1‑149 (MQ=255)
                                                                                                                          tAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTg                    <  3:2893/148‑1 (MQ=255)
                                                                                                                             gCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTAt                >  6:351402/1‑149 (MQ=255)
                                                                                                                               tATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGATGGTAttt              >  3:159251/1‑149 (MQ=255)
                                                                                                                                  ttagggCCGCTTCGCGGCCCTAATTTCCACCGGATGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGTCAAACCCCCCCAGGCAGGTAATGGACAAACGCAAACCGCCGAGTTCGCTCTGGTAtttag            >  6:415137/4‑146 (MQ=255)
                                                                                                                                    ggggCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTa         >  7:178626/1‑149 (MQ=255)
                                                                                                                                     gggCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTa         <  4:159251/148‑1 (MQ=255)
                                                                                                                                         cgcgAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCAGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTAcgcg     >  1:124283/1‑148 (MQ=255)
                                                                                                                                         cgcgAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCATAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTACgcgc    >  1:72645/1‑149 (MQ=255)
                                                                                                                                          gcgAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTACGCGCt   >  3:105803/1‑149 (MQ=255)
                                                                                                                                          gcgAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTACGCGCt   <  8:315267/149‑1 (MQ=255)
                                                                                                                                           cgAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTACGCGCTc  <  3:46677/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
TCGAGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTACGCGCTC  >  NC_002947/2052644‑2052931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑