Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,068,151	A→T	7.1%	intergenic (‑96/+25)	plsC ← / ← PP_1845	1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase/enoyl‑CoA hydratase/isomerase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,068,151	0	A	T	7.1%	203.8 / 3.4	69	intergenic (‑96/+25)	plsC/PP_1845	1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase/enoyl‑CoA hydratase/isomerase family protein
Reads supporting (aligned to +/- strand):  ref base A (30/34);  new base T (3/2);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAGCTCCCGC  >  NC_002947/2068017‑2068293                                                                                                                                        |                                                                                                                                                aTGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAgg                                                                                                                                   <  1:290701/149‑1 (MQ=255) aTGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAgg                                                                                                                                   <  4:140787/149‑1 (MQ=255)   gCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGc                                                                                                                                 >  8:85685/1‑149 (MQ=255)       ccccAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGt                                                                                                                             >  4:20887/1‑149 (MQ=255)         ccAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGCCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGc                                                                                                                           >  3:374938/1‑149 (MQ=255)          cagcagGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCg                                                                                                                          >  4:392015/1‑149 (MQ=255)          cagcagGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCg                                                                                                                          <  7:85685/149‑1 (MQ=255)           agcagGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCgg                                                                                                                         <  3:370346/149‑1 (MQ=255)              agGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGCCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTa                                                                                                                      <  4:374938/149‑1 (MQ=255)                gAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGc                                                                                                                    <  1:302651/149‑1 (MQ=255)                   cATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCaa                                                                                                                 >  7:304781/1‑149 (MQ=255)                        gCAATGAATATCCCATTGTACGACGCACACCAGACGGTCGCGGGGCAGTGGACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAt‑gc                                                                                                           <  8:254080/149‑1 (MQ=255)                           aTGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAt‑gctgc                                                                                                        <  8:304781/149‑1 (MQ=255)                            tGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑gctgct                                                                                                       >  2:224091/1‑149 (MQ=255)                               aTAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGAt                                                                                                    <  3:204789/149‑1 (MQ=255)                                tAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATc                                                                                                   >  8:96120/1‑149 (MQ=255)                                     cATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCt                                                                                              <  3:392015/149‑1 (MQ=255)                                            cgtcTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCAGTGGCTTGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGc                                                                                       <  1:62996/146‑1 (MQ=255)                                              aCTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCt                                                                                     <  5:199911/149‑1 (MQ=255)                                               cTCACACCAGACGGGCGCGGGGCAGTGTACGGGGGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCtt                                                                                    <  1:53618/149‑1 (MQ=255)                                                tCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCt                                                                                              >  4:397035/1‑138 (MQ=255)                                                tCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCt                                                                                              <  3:397035/138‑1 (MQ=255)                                                tCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTg                                                                                   >  7:110652/1‑149 (MQ=255)                                                 cacaCCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGt                                                                                  >  4:383431/1‑149 (MQ=255)                                                  acacCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTc                                                                                 >  7:356953/1‑149 (MQ=255)                                                  acacCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTc                                                                                 <  2:335050/149‑1 (MQ=255)                                                    acCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCa                                                                               >  3:7978/1‑149 (MQ=255)                                                     ccAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGa                                                                                                     >  7:71508/1‑126 (MQ=255)                                                     ccAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGa                                                                                                     <  8:71508/126‑1 (MQ=255)                                                      cAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAgc                                                                             >  4:270672/1‑149 (MQ=255)                                                      cAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAgc                                                                             <  1:224091/149‑1 (MQ=255)                                                        gACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCCTTTTCGCGGGCTTGCCCGCGaaa                                                                                                                                      >  6:119133/1‑92 (MQ=38)                                                        gACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCCTTTTCGCGGGCTTGCCCGCGaaa                                                                                                                                      <  5:119133/92‑1 (MQ=38)                                                         aCGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGcc                                                                          >  2:100840/1‑149 (MQ=255)                                                           ggTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCT‑TTTCGCGGGCTTGCCCGCGAAAgggc                                                                                                                                  >  6:419759/1‑91 (MQ=37)                                                           ggTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTc                                                                        <  3:20887/149‑1 (MQ=255)                                                           ggTCGCGGGGCAGTCTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTc                                                                        <  8:230555/149‑1 (MQ=255)                                                             tCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTc                                                                        >  5:155520/1‑147 (MQ=255)                                                                 ggggCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCcagcag                                                                  >  4:243831/1‑149 (MQ=255)                                                                  gggCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCagcagc                                                                 <  3:383431/149‑1 (MQ=255)                                                                  gggCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCagcagc                                                                 <  8:356953/149‑1 (MQ=255)                                                                          tACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATAGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTg                                                           <  1:100840/148‑1 (MQ=255)                                                                             gggTGTGCACTGTCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCAc                                                      >  8:341587/1‑149 (MQ=255)                                                                             gggTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCACGTGCGGGTAGCCTATAT‑GCTGCTTATCAAGCTCGATCGCCTTGTCCACCGCCTCCACCAGCCCTATGAGCAc                                                      >  7:304678/1‑149 (MQ=255)                                                                                 gtgCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTc                                                  <  3:270672/149‑1 (MQ=255)                                                                                  tgCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTc                                                  <  7:96120/148‑1 (MQ=255)                                                                                    cACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCACCCCTTTGCGCACTTTCAGc                                               >  6:296723/1‑149 (MQ=255)                                                                                         ggtggGGCATACCTTGGTGGAGGCCTGTTACGGCCC‑ATTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑CCTGCTGATCCAGCTCGATCGCCTTGTCCAGCTCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTg                                            <  6:174533/145‑1 (MQ=255)                                                                                         gctggGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTg                                            <  3:243831/147‑1 (MQ=255)                                                                                              ggCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCt                                     >  2:96886/1‑149 (MQ=255)                                                                                               ggaaaCCTTGGTGCAGGCCTGTGACGGCCC‑TTTCGCGCGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCTGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCtt                                    <  6:332341/147‑1 (MQ=255)                                                                                               gCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCtt                                    <  7:341587/149‑1 (MQ=255)                                                                                                             aGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTtgt                                  <  8:379916/137‑1 (MQ=255)                                                                                                             aGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTtgt                                  >  7:379916/1‑137 (MQ=255)                                                                                                               gCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGCTCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGtt                     >  2:190352/1‑148 (MQ=255)                                                                                                               gCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTc                    <  8:110652/149‑1 (MQ=255)                                                                                                                 cTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTg                                            <  4:236649/123‑1 (MQ=255)                                                                                                                 cTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTg                                            >  3:236649/1‑123 (MQ=255)                                                                                                                       cGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTg                                            <  8:229086/117‑1 (MQ=255)                                                                                                                       cGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTg                                            >  7:229086/1‑117 (MQ=255)                                                                                                                        ggCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCttcttc           <  7:355965/149‑1 (MQ=255)                                                                                                                         gCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGcttctt            >  7:31600/1‑147 (MQ=255)                                                                                                                         gCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGcttctt            >  1:18151/1‑147 (MQ=255)                                                                                                                           ccttttCGCGGGCTTGCCCGCG‑AAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGTCCTTTGCGCACTTTCAGCTTGGTGTTCtt                                    <  8:134207/118‑1 (MQ=39)                                                                                                                           ccttttCGCGGGCTTGCCCGCG‑AAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGTCCTTTGCGCACTTTCAGCTTGGTGTTCtt                                    >  7:134207/4‑121 (MQ=39)                                                                                                                           cc‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCtt                                    <  6:170917/121‑1 (MQ=255)                                                                                                                           cc‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCtt                                    >  5:170917/1‑121 (MQ=255)                                                                                                                           cc‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAGc        >  1:403442/1‑149 (MQ=255)                                                                                                                                  gcgGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAGCTCCcgc  >  6:162139/1‑149 (MQ=255)                                                                                                                                        aaGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGcttc              >  3:130440/1‑131 (MQ=255)                                                                                                                                        aaGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGcttc              <  4:130440/131‑1 (MQ=255)                                                                                                                                        |                                                                                                                                                ATGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAT‑GCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAGCTCCCGC  >  NC_002947/2068017‑2068293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑