Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,090,928 A→G 5.7% E85G (GAG→GGG)  deaD → ATP‑dependent DEAD‑box RNA helicase DeaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,090,9280AG 5.7% 209.5 / 2.2 70E85G (GAG→GGG) deaDATP‑dependent DEAD‑box RNA helicase DeaD
Reads supporting (aligned to +/- strand):  ref base A (37/29);  new base G (2/2);  total (39/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

GATCCCGATCATCCTCGCCGGTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGGCGCGCAAATCGTCGTGGCCACCCCG  >  NC_002947/2090782‑2091073
                                                                                                                                                  |                                                                                                                                                 
gATCCCGATCATCCTCGCCGGTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCACCCCGTGAGt                                                                                                                                                 >  8:347882/1‑149 (MQ=255)
gATCCCGATCATCCTCGCCGGTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGt                                                                                                                                                 >  2:130518/1‑149 (MQ=255)
  tCCCGATCATCCTCGCCGGTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTg                                                                                                                                               <  3:315959/149‑1 (MQ=255)
    ccGATCATCCTCGCCGGTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTgg                                                                                                                                              <  7:210830/148‑1 (MQ=255)
     cGATCATCCTCGCCGGTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACTCACGGGTTGgcg                                                                                                                                            <  8:137877/149‑1 (MQ=255)
       atcatcCTCGCCGGTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCt                                                                                                                                          <  2:13596/149‑1 (MQ=255)
          atcCTCGCCGGTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACTCACGGGTTGgcgccagg                                                                                                                                       <  8:159767/149‑5 (MQ=255)
              tCGCCGGTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGtt                                                                                                                                   >  7:29084/1‑149 (MQ=255)
                gCCGGTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGtt                                                                                                                                   <  2:257252/147‑1 (MQ=255)
                gCCGGTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGtt                                                                                                                                   <  6:365334/147‑1 (MQ=255)
                gCCGGTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGc                                                                                                                                 <  3:326420/149‑1 (MQ=255)
                  cGGTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTa                                                                                                                               >  3:215509/1‑149 (MQ=255)
                    gTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTAcc                                                                                                                             <  1:257763/149‑1 (MQ=255)
                       aCGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCt                                                                                                                          >  1:46041/1‑149 (MQ=255)
                         gACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCttt                                                                                                                        >  4:177931/1‑149 (MQ=255)
                         gACATGATCGGCCAGGCGCAGACTGGCACAGGCCAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCACGCGCGAACCGCACGCCCTGATCCTGGCGCCAACCCGGGAGTTGGCGCTGCAAGTTGCTACCGCttt                                                                                                                        >  8:262613/1‑149 (MQ=255)
                            aTGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCg                                                                                                                      >  5:105729/1‑148 (MQ=255)
                             tGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGaa                                                                                                                    <  8:29084/149‑1 (MQ=255)
                               aTCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAAc                                                                                                                  >  6:241289/1‑149 (MQ=255)
                                tCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAAcc                                                                                                                 >  5:88462/1‑149 (MQ=255)
                                     cAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGc                                                                                                            <  3:142801/149‑1 (MQ=255)
                                      aGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGc                                                                                                            >  5:384076/1‑148 (MQ=255)
                                        gcgcAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCaa                                                                                                         <  5:326702/149‑1 (MQ=255)
                                         cgcAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAg                                                                                                        <  3:177931/149‑1 (MQ=255)
                                          gcAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGc                                                                                                       <  6:384076/149‑1 (MQ=255)
                                           cAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCa                                                                                                      <  2:46041/149‑1 (MQ=255)
                                           cAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCa                                                                                                      <  4:174285/149‑1 (MQ=255)
                                             gACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGa                                                                                                    <  4:215509/149‑1 (MQ=255)
                                             gACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGa                                                                                                    <  5:241289/149‑1 (MQ=255)
                                              aCTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGAt                                                                                                   >  7:328959/1‑149 (MQ=255)
                                                tGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGaaa                                                                                                                   >  3:324070/1‑131 (MQ=255)
                                                tGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGaaa                                                                                                                   <  4:324070/131‑1 (MQ=255)
                                                    aCCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGGACAAGATCGATGTGCGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTCCCGCTGTCGAAACCTACGCCCAGCAGCTGCCggg                                                                                             >  2:396754/1‑149 (MQ=255)
                                                       ggCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGt                                                                                          <  1:238477/149‑1 (MQ=255)
                                                        gCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGt                                                                                          >  4:152718/1‑148 (MQ=255)
                                                           aacccGCTGCCTTCGCCCTGCCGATCCTGGACAAGATCGATGTGAGCACGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAAc                                                                                      <  1:396754/146‑1 (MQ=255)
                                                               cGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTg                                                                                  >  4:383814/1‑149 (MQ=255)
                                                                    ccTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGcc                                                                             >  3:37992/1‑149 (MQ=255)
                                                                      ttCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGt                                                                           >  3:15691/1‑149 (MQ=255)
                                                                         gCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTATGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCt                                                                         <  6:88462/148‑1 (MQ=255)
                                                                         gCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTa                                                                        >  6:42258/1‑149 (MQ=255)
                                                                          cccTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTAc                                                                       >  2:313603/1‑149 (MQ=255)
                                                                               ccGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAg                                                                                                        >  6:310958/1‑111 (MQ=255)
                                                                                       gAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGa                                                          >  3:8291/1‑149 (MQ=255)
                                                                                          cAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATgg                                                       <  8:354850/149‑1 (MQ=255)
                                                                                           aaGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATggg                                                      <  3:383814/149‑1 (MQ=255)
                                                                                            aGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGc                                                     <  4:37992/149‑1 (MQ=255)
                                                                                                  aTGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAg                                               <  2:379892/149‑1 (MQ=255)
                                                                                                            gcgcGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTc                                                                                <  3:330703/106‑1 (MQ=255)
                                                                                                            gcgcGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTc                                                                                >  4:330703/1‑106 (MQ=255)
                                                                                                            gcgcGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGa                                     >  1:177303/1‑149 (MQ=255)
                                                                                                            gcgcGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGa                                     >  3:284078/1‑149 (MQ=255)
                                                                                                              gcgAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATc                                   <  5:42258/149‑1 (MQ=255)
                                                                                                                     gCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACg                            <  1:313603/149‑1 (MQ=255)
                                                                                                                          cccTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGAAGGGCCCACAGCTGCGTGCGATCCGCAACGgcgcg                       >  7:125932/1‑149 (MQ=255)
                                                                                                                           ccTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGgcgcg                       >  6:78275/1‑148 (MQ=255)
                                                                                                                           ccTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGgcgcg                       >  7:222123/1‑148 (MQ=255)
                                                                                                                           ccTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGgcgcg                       >  8:219686/1‑148 (MQ=255)
                                                                                                                                tCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGGCGCGCAAAtc                 <  7:219686/149‑1 (MQ=255)
                                                                                                                                 ccTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGGCGCGCAAAtcg                >  3:330659/1‑149 (MQ=255)
                                                                                                                                    ggCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGGCGCGCAAAtcgtcg             >  7:370553/1‑149 (MQ=255)
                                                                                                                                     gcgcCAACTCACGGGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATgg                                                       >  6:340687/1‑106 (MQ=255)
                                                                                                                                     gcgcCAACTCACGGGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGGCGCGCAAATcgtcgt            >  7:72588/1‑149 (MQ=255)
                                                                                                                                          aaCCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGGCGCGCAAATCGTCGTGGCCa       >  7:32343/1‑149 (MQ=255)
                                                                                                                                             ccGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGgc                          >  7:224217/1‑127 (MQ=255)
                                                                                                                                             ccGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGgc                          <  8:224217/127‑1 (MQ=255)
                                                                                                                                             ccGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGGCGCGCAAATCGTCGTGGCCAccc    >  7:165621/1‑149 (MQ=255)
                                                                                                                                              cGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGGCGCGCAAATCGTCGTGGCCAcccc   >  8:11667/1‑149 (MQ=255)
                                                                                                                                               gTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGGCGCGCAAATCGTCGTGGCCACCCCg  >  6:338479/1‑149 (MQ=255)
                                                                                                                                               gTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGGCGCGCAAATCGTCGTGGCCACCCCg  >  8:98999/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GATCCCGATCATCCTCGCCGGTCACGACATGATCGGCCAGGCGCAGACTGGCACCGGCAAAACCGCTGCCTTCGCCCTGCCGATCCTGAACAAGATCGATGTGAGCAAGCGCGAACCGCAAGCCCTGATCCTGGCGCCAACCCGTGAGTTGGCGCTGCAAGTTGCTACCGCTTTCGAAACCTACGCCAAGCAGATGCCGGGCGTGAACGTTGTCGCCGTCTACGGTGGTGCCCCGATGGGCCCACAGCTGCGTGCGATCCGCAACGGCGCGCAAATCGTCGTGGCCACCCCG  >  NC_002947/2090782‑2091073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑