Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,131,450	G→T	5.3%	T250K (ACG→AAG) ‡	fimD ←	type I pili subunit FimD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,131,450	0	G	T	5.3%	248.2 / 3.5	76	T250K (ACG→AAG) ‡	fimD	type I pili subunit FimD
Reads supporting (aligned to +/- strand):  ref base G (33/39);  new base T (2/2);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-01

GACTTTCGCCCGGCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAGGTACAGG  >  NC_002947/2131302‑2131596                                                                                                                                                     |                                                                                                                                                   gACTTTCGCCCGGCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCg                                                                                                                                                    >  2:272697/1‑149 (MQ=255)    tttCGCCCGGCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGGTATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGATTCGCCAATCGt                                                                                                                                                   >  3:305028/1‑147 (MQ=255)        gCCCGGCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGAtt                                                                                                                                             <  4:37698/149‑1 (MQ=255)        gCCCGGCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGACACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGAt                                                                                                                                              <  1:293888/148‑1 (MQ=255)            ggCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCg                                                                                                                                         <  7:57875/149‑1 (MQ=255)             gCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGa                                                                                                                                        <  2:360407/149‑1 (MQ=255)              ctctGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAg                                                                                                                                       <  8:204652/149‑1 (MQ=255)                   ggCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCTGACGATTGGCGaaac                                                                                                                                     <  2:29159/146‑4 (MQ=255)                    gCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTAcc                                                                                                                                 >  2:248548/1‑149 (MQ=255)                             cgAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATc                                                                                                                        <  2:197520/149‑1 (MQ=255)                             cgAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATc                                                                                                                        <  7:320328/149‑1 (MQ=255)                             cgAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATc                                                                                                                        >  5:144806/1‑149 (MQ=255)                              gAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATcg                                                                                                                       <  4:154297/149‑1 (MQ=255)                                aTGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATcgcg                                                                                                                     <  2:144476/149‑1 (MQ=255)                                aTGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATcgcg                                                                                                                     <  2:344350/149‑1 (MQ=255)                                  gATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCt                                                                                                                   >  8:99779/1‑149 (MQ=255)                                    tCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCtgt                                                                                                                 >  6:157367/1‑149 (MQ=255)                                      ggggCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCTGACGATTGGCGaaac                                                                                                                                     <  2:356762/127‑4 (MQ=255)                                      ggggCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCTGACGATTGGCGaaac                                                                                                                                     >  1:356762/1‑124 (MQ=255)                                          cATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAg                                                                                                           <  3:237839/149‑1 (MQ=255)                                          cATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAg                                                                                                           <  1:272697/149‑1 (MQ=255)                                           aTAACCGCGCTGGTTGTCGGCCAGCATGCCCGTGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCg                                                                                                              <  4:341779/145‑1 (MQ=255)                                           aTAACCGCGCTGGTTGTCGGCCAGCATGCCCGTGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCg                                                                                                              <  4:335968/145‑1 (MQ=255)                                           aTAACCGCGCTGGTTGTCGGCCAGCATGCCCGTGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCg                                                                                                              >  3:335968/1‑145 (MQ=255)                                           aTAACCGCGCTGGTTGTCGGCCAGCATGCCCGTGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCg                                                                                                              >  3:341779/1‑145 (MQ=255)                                              aCCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATc                                                                                                                        <  8:238746/132‑1 (MQ=255)                                              aCCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATc                                                                                                                        >  7:238746/1‑132 (MQ=255)                                                     tGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCg                                                                                                >  2:300590/1‑149 (MQ=255)                                                       gTTGTCGGCCAGCATGCAGCTGTCCGTGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCTTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCgg                                                                                               <  4:305028/148‑1 (MQ=255)                                                             ggCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCAtt                                                                                        <  3:316154/149‑1 (MQ=255)                                                                 aGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAg                                                                                     >  4:151717/1‑148 (MQ=255)                                                                 aGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGt                                                                                    <  3:97161/149‑1 (MQ=255)                                                                  gCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACCCCTCCCTGTCCGTGAAGGTTTCGCCAATCGGCAGATTGGCGAGGGTACCCGGGATATCGCGCTGTGCGTAGGTCTGTGCCCGGGGCCATTCAGTc                                                                                   >  7:101131/1‑149 (MQ=255)                                                                     tGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTtg                                                                                <  5:245191/149‑1 (MQ=255)                                                                        ccATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCAtt                                                                                        <  7:172080/138‑1 (MQ=255)                                                                        ccATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCAtt                                                                                        >  8:172080/1‑138 (MQ=255)                                                                          aTGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTtgccctg                                                                           <  2:395755/149‑1 (MQ=255)                                                                              ccGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTCTGCCCGGGTCCATTCAGTCTtgccctgcctg                                                                       >  3:240568/1‑149 (MQ=255)                                                                               cGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTtgccctggctgc                                                                      >  4:171880/1‑149 (MQ=255)                                                                                gAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTtgccctggctgcc                                                                     >  7:14336/1‑149 (MQ=255)                                                                                gAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGGCTGTGCCCGGGTCCATTCAGTCTtgccctggctgcc                                                                     <  6:340616/149‑1 (MQ=255)                                                                                     cACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTtgccctggctgccctggc                                                                >  5:232955/1‑149 (MQ=255)                                                                                      aCGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGgcg                                                               >  8:258267/1‑149 (MQ=255)                                                                                               ccTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTg                                                      <  3:171880/149‑1 (MQ=255)                                                                                                cTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTgg                                                     <  8:14336/149‑1 (MQ=255)                                                                                                 tGATATCGGCACCGAACGGAACACGTCCCTGTCCGTGAAGTTTTCTCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCtgt                                                                                                                 <  1:13711/88‑1 (MQ=255)                                                                                                 tGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTAAGATTGGCGAGGGTACGCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCTGTCTTGCCCTGGCTGAGCTGGCGCCAGGACTggg                                                    >  6:128885/1‑149 (MQ=255)                                                                                                 tGATATCGGCACCGAACGGAACACCTCCCAGTCCGTGAAGGTTTCGCCAAACGTCAGATTGGCGAGGGTACCCGGTATATCGCGCtgt                                                                                                                 >  2:13711/1‑88 (MQ=255)                                                                                                   atatCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTggg                                                    <  3:151717/147‑1 (MQ=255)                                                                                                   atatCGGCACCGAACGGAACACCTCCCTGTCCGTGAACGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTgg                                                     <  5:128885/146‑1 (MQ=255)                                                                                                      tCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTg                                               <  1:248548/149‑1 (MQ=255)                                                                                                           aCCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAg                                          <  1:300590/149‑1 (MQ=255)                                                                                                           aCCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAg                                          >  6:171622/1‑149 (MQ=255)                                                                                                                  ggAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCAt                                   >  4:309361/1‑149 (MQ=255)                                                                                                                       acCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGcc                              <  5:171622/149‑1 (MQ=255)                                                                                                                         cTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGcc                              >  6:325822/1‑147 (MQ=255)                                                                                                                            ccTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTtgccctggctgccctggc                                                                <  4:48659/110‑1 (MQ=255)                                                                                                                            ccTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTtgccctggctgccctggc                                                                >  3:48659/1‑110 (MQ=255)                                                                                                                            ccTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGt                         >  6:78531/1‑149 (MQ=255)                                                                                                                             cTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGtt                        <  6:144806/149‑1 (MQ=255)                                                                                                                             cTGTCCGGGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGGCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGtt                        <  8:101131/149‑1 (MQ=255)                                                                                                                                tCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGa                      >  2:136832/1‑148 (MQ=255)                                                                                                                                tCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGAt                     <  6:232955/149‑1 (MQ=255)                                                                                                                                tCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGATACTGCGCAGGCGCCATTCGCCCAGGTTGa                      >  6:80038/1‑148 (MQ=255)                                                                                                                                  cGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCAt                                                                                         <  4:111468/79‑1 (MQ=255)                                                                                                                                  cGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCAt                                                                                         >  3:111468/1‑79 (MQ=255)                                                                                                                                  cGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATAc                   >  6:105941/1‑149 (MQ=255)                                                                                                                                    tGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCa                 >  3:130898/1‑149 (MQ=255)                                                                                                                                     gAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGACCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCAc                >  3:23118/1‑149 (MQ=255)                                                                                                                                      aaGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACt               <  5:157367/149‑1 (MQ=255)                                                                                                                                            tCGCCAATCTGACGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAg         >  3:334675/1‑149 (MQ=255)                                                                                                                                              gCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGTCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAgg        >  8:128053/1‑148 (MQ=255)                                                                                                                                              gCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAgg        >  7:123796/1‑148 (MQ=255)                                                                                                                                               ccAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCTGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAGGTa      <  7:258267/149‑1 (MQ=255)                                                                                                                                                  aTCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAGGTACAg   <  5:325822/149‑1 (MQ=255)                                                                                                                                                   tCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAGGTACAgg  <  5:105941/149‑1 (MQ=255)                                                                                                                                                   tCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGACCTGGCTGCCCTGGCGCCAGGACTGGGTACTGAGCAGGCGCCATTCGCCCAGGTTGCTACAACTGTTCAGGTACAgg  >  3:246827/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GACTTTCGCCCGGCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAGGTACAGG  >  NC_002947/2131302‑2131596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑