Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,131,451	T→G	6.4%	T250P (ACG→CCG) ‡	fimD ←	type I pili subunit FimD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,131,451	0	T	G	6.4%	224.6 / 4.8	75	T250P (ACG→CCG) ‡	fimD	type I pili subunit FimD
Reads supporting (aligned to +/- strand):  ref base T (31/39);  new base G (3/2);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

TTTCGCCCGGCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAGGTACAGG  >  NC_002947/2131305‑2131596                                                                                                                                                   |                                                                                                                                                  tttCGCCCGGCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGGTATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGATTCGCCAATCGt                                                                                                                                                   >  3:305028/1‑147 (MQ=255)     gCCCGGCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGAtt                                                                                                                                             <  4:37698/149‑1 (MQ=255)     gCCCGGCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGACACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGAt                                                                                                                                              <  1:293888/148‑1 (MQ=255)         ggCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCg                                                                                                                                         <  7:57875/149‑1 (MQ=255)          gCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGa                                                                                                                                        <  2:360407/149‑1 (MQ=255)           ctctGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAg                                                                                                                                       <  8:204652/149‑1 (MQ=255)                ggCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCTGACGATTGGCGaaac                                                                                                                                     <  2:29159/146‑4 (MQ=255)                 gCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTAcc                                                                                                                                 >  2:248548/1‑149 (MQ=255)                          cgAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATc                                                                                                                        <  7:320328/149‑1 (MQ=255)                          cgAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATc                                                                                                                        <  2:197520/149‑1 (MQ=255)                          cgAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATc                                                                                                                        >  5:144806/1‑149 (MQ=255)                           gAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATcg                                                                                                                       <  4:154297/149‑1 (MQ=255)                             aTGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATcgcg                                                                                                                     <  2:144476/149‑1 (MQ=255)                             aTGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATcgcg                                                                                                                     <  2:344350/149‑1 (MQ=255)                               gATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCt                                                                                                                   >  8:99779/1‑149 (MQ=255)                                 tCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCtgt                                                                                                                 >  6:157367/1‑149 (MQ=255)                                   ggggCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCTGACGATTGGCGaaac                                                                                                                                     <  2:356762/127‑4 (MQ=255)                                   ggggCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCTGACGATTGGCGaaac                                                                                                                                     >  1:356762/1‑124 (MQ=255)                                       cATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAg                                                                                                           <  1:272697/149‑1 (MQ=255)                                       cATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAg                                                                                                           <  3:237839/149‑1 (MQ=255)                                        aTAACCGCGCTGGTTGTCGGCCAGCATGCCCGTGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCg                                                                                                              <  4:341779/145‑1 (MQ=255)                                        aTAACCGCGCTGGTTGTCGGCCAGCATGCCCGTGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCg                                                                                                              >  3:341779/1‑145 (MQ=255)                                        aTAACCGCGCTGGTTGTCGGCCAGCATGCCCGTGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCg                                                                                                              >  3:335968/1‑145 (MQ=255)                                        aTAACCGCGCTGGTTGTCGGCCAGCATGCCCGTGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCg                                                                                                              <  4:335968/145‑1 (MQ=255)                                           aCCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATc                                                                                                                        <  8:238746/132‑1 (MQ=255)                                           aCCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATc                                                                                                                        >  7:238746/1‑132 (MQ=255)                                                  tGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCg                                                                                                >  2:300590/1‑149 (MQ=255)                                                    gTTGTCGGCCAGCATGCAGCTGTCCGTGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCTTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCgg                                                                                               <  4:305028/148‑1 (MQ=255)                                                          ggCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCAtt                                                                                        <  3:316154/149‑1 (MQ=255)                                                              aGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAg                                                                                     >  4:151717/1‑148 (MQ=255)                                                              aGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGt                                                                                    <  3:97161/149‑1 (MQ=255)                                                               gCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACCCCTCCCTGTCCGTGAAGGTTTCGCCAATCGGCAGATTGGCGAGGGTACCCGGGATATCGCGCTGTGCGTAGGTCTGTGCCCGGGGCCATTCAGTc                                                                                   >  7:101131/1‑149 (MQ=255)                                                                  tGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTtg                                                                                <  5:245191/149‑1 (MQ=255)                                                                     ccATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCAtt                                                                                        >  8:172080/1‑138 (MQ=255)                                                                     ccATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCAtt                                                                                        <  7:172080/138‑1 (MQ=255)                                                                       aTGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTtgccctg                                                                           <  2:395755/149‑1 (MQ=255)                                                                           ccGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTCTGCCCGGGTCCATTCAGTCTtgccctgcctg                                                                       >  3:240568/1‑149 (MQ=255)                                                                            cGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTtgccctggctgc                                                                      >  4:171880/1‑149 (MQ=255)                                                                             gAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTtgccctggctgcc                                                                     >  7:14336/1‑149 (MQ=255)                                                                             gAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGGCTGTGCCCGGGTCCATTCAGTCTtgccctggctgcc                                                                     <  6:340616/149‑1 (MQ=255)                                                                                  cACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTtgccctggctgccctggc                                                                >  5:232955/1‑149 (MQ=255)                                                                                   aCGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGgcg                                                               >  8:258267/1‑149 (MQ=255)                                                                                            ccTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTg                                                      <  3:171880/149‑1 (MQ=255)                                                                                             cTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTgg                                                     <  8:14336/149‑1 (MQ=255)                                                                                              tGATATCGGCACCGAACGGAACACGTCCCTGTCCGTGAAGTTTTCTCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCtgt                                                                                                                 <  1:13711/88‑1 (MQ=255)                                                                                              tGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTAAGATTGGCGAGGGTACGCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCTGTCTTGCCCTGGCTGAGCTGGCGCCAGGACTggg                                                    >  6:128885/1‑149 (MQ=255)                                                                                              tGATATCGGCACCGAACGGAACACCTCCCAGTCCGTGAAGGTTTCGCCAAACGTCAGATTGGCGAGGGTACCCGGTATATCGCGCtgt                                                                                                                 >  2:13711/1‑88 (MQ=255)                                                                                                atatCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTggg                                                    <  3:151717/147‑1 (MQ=255)                                                                                                atatCGGCACCGAACGGAACACCTCCCTGTCCGTGAACGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTgg                                                     <  5:128885/146‑1 (MQ=255)                                                                                                   tCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTg                                               <  1:248548/149‑1 (MQ=255)                                                                                                        aCCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAg                                          <  1:300590/149‑1 (MQ=255)                                                                                                        aCCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAg                                          >  6:171622/1‑149 (MQ=255)                                                                                                               ggAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCAt                                   >  4:309361/1‑149 (MQ=255)                                                                                                                    acCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGcc                              <  5:171622/149‑1 (MQ=255)                                                                                                                      cTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGcc                              >  6:325822/1‑147 (MQ=255)                                                                                                                         ccTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTtgccctggctgccctggc                                                                >  3:48659/1‑110 (MQ=255)                                                                                                                         ccTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTtgccctggctgccctggc                                                                <  4:48659/110‑1 (MQ=255)                                                                                                                         ccTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGt                         >  6:78531/1‑149 (MQ=255)                                                                                                                          cTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGtt                        <  6:144806/149‑1 (MQ=255)                                                                                                                          cTGTCCGGGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGGCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGtt                        <  8:101131/149‑1 (MQ=255)                                                                                                                             tCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGa                      >  2:136832/1‑148 (MQ=255)                                                                                                                             tCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGAt                     <  6:232955/149‑1 (MQ=255)                                                                                                                             tCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGATACTGCGCAGGCGCCATTCGCCCAGGTTGa                      >  6:80038/1‑148 (MQ=255)                                                                                                                               cGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCAt                                                                                         <  4:111468/79‑1 (MQ=255)                                                                                                                               cGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCAt                                                                                         >  3:111468/1‑79 (MQ=255)                                                                                                                               cGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATAc                   >  6:105941/1‑149 (MQ=255)                                                                                                                                 tGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCa                 >  3:130898/1‑149 (MQ=255)                                                                                                                                  gAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGACCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCAc                >  3:23118/1‑149 (MQ=255)                                                                                                                                   aaGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACt               <  5:157367/149‑1 (MQ=255)                                                                                                                                         tCGCCAATCTGACGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAg         >  3:334675/1‑149 (MQ=255)                                                                                                                                           gCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGTCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAgg        >  8:128053/1‑148 (MQ=255)                                                                                                                                           gCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAgg        >  7:123796/1‑148 (MQ=255)                                                                                                                                            ccAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCTGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAGGTa      <  7:258267/149‑1 (MQ=255)                                                                                                                                               aTCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAGGTACAg   <  5:325822/149‑1 (MQ=255)                                                                                                                                                tCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAGGTACAgg  <  5:105941/149‑1 (MQ=255)                                                                                                                                                tCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGACCTGGCTGCCCTGGCGCCAGGACTGGGTACTGAGCAGGCGCCATTCGCCCAGGTTGCTACAACTGTTCAGGTACAgg  >  3:246827/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                  TTTCGCCCGGCTCTGGGCCACGCCGCGAATGATCGGGGCATAACCGCGCTGGTTGTCGGCCAGCATGCCCATGTCCGAGGCCACGCGCACGCCTGATATCGGCACCGAACGGAACACCTCCCTGTCCGTGAAGGTTTCGCCAATCGTCAGATTGGCGAGGGTACCCGGTATATCGCGCTGTGCGTAGGTCTGTGCCCGGGTCCATTCAGTCTTGCCCTGGCTGCCCTGGCGCCAGGACTGGGTACTGCGCAGGCGCCATTCGCCCAGGTTGATACCACTGTTCAGGTACAGG  >  NC_002947/2131305‑2131596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑