Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,242,037	G→A	7.1%	intergenic (+35/‑27)	PP_t28 → / → PP_t29	tRNA‑Glu/tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,242,037	0	G	A	7.1%	169.1 / 2.0	56	intergenic (+35/‑27)	PP_t28/PP_t29	tRNA‑Glu/tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base G (22/30);  new base A (2/2);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

TGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGT  >  NC_002947/2241889‑2242183                                                                                                                                                     |                                                                                                                                                   tGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCtcg                                                                                                                                                    >  5:226064/1‑149 (MQ=255)    cgcgGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCtcgtc                                                                                                                                                  >  1:114503/1‑148 (MQ=255)           cgcgTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGattatt                                                                                                                                           <  3:196929/148‑1 (MQ=255)            gcgTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTT‑AAAAGGCTCGTCGATTATtgat                                                                                                                                        <  5:79820/149‑1 (MQ=255)               ttAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATtgatga                                                                                                                                      <  6:2861/149‑1 (MQ=255)                     tatgtgCACGCAGGTTACTTGCCTTTAGTATAGTTCCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCATCAATCATCGACGAGCCttt                                                                                                                                <  7:346492/144‑1 (MQ=17)                     gATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCttt                                                                                                                                >  8:188610/1‑149 (MQ=255)                      aTCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCtttt                                                                                                                               <  3:68975/149‑1 (MQ=255)                             cGAAGGTTACGTCCCCTTCGTCTAGTGTCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCgg                                                                                                                        <  2:309011/149‑1 (MQ=255)                              gAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCggg                                                                                                                       <  1:364203/149‑1 (MQ=255)                                aGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGc                                                                                                                     <  7:125604/149‑1 (MQ=255)                                   ttACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCta                                                                                                                   >  5:152917/1‑148 (MQ=255)                                    tACGTCCCCTTCGTTTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCATCAATAATCGACGAGCCtttttaa                                                                                                                            <  1:15859/138‑4 (MQ=17)                                    tACGTCCCCTTCGTTTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCATCAATAATCGACGAGCCtttttaa                                                                                                                            >  2:15859/1‑135 (MQ=17)                                       gTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCt                                                                                                              <  7:111750/149‑1 (MQ=255)                                          cccTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAg                                                                                                           >  7:113981/1‑149 (MQ=255)                                            cTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCt                                                                                                         >  2:86079/1‑149 (MQ=255)                                               cGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTgg                                                                                                       <  6:152917/148‑1 (MQ=255)                                                       ggCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACACCACTTTGCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAgcgc                                                                                               <  7:262379/148‑1 (MQ=255)                                                        gCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTgg                                                                                                       <  4:89908/139‑1 (MQ=255)                                                        gCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTgg                                                                                                       >  3:89908/1‑139 (MQ=255)                                                        gCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCt                                                                                             >  2:181279/1‑149 (MQ=255)                                                        gCCTAGGACACAGCCCTTTCACGGCGGTAACAGGCGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCATGGCTTAAAAAGGCTCGTCGATTATTGAAGAGACTTTTGTTTCGGGGCTATAGCTCACCTGGGAGAACGCCt                                                                                             >  3:236954/1‑149 (MQ=25)                                                           tAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgca                                                                                          >  1:290672/1‑149 (MQ=255)                                                             ggACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcat                                                                                         <  8:113981/148‑1 (MQ=255)                                                             ggACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgca                                                                                          <  8:191808/147‑1 (MQ=255)                                                               acacCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatggc                                                                                      <  6:189563/149‑1 (MQ=255)                                                                 acCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatggcat                                                                                    >  4:38563/1‑149 (MQ=255)                                                                 acCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatggcat                                                                                    >  5:47909/1‑149 (MQ=255)                                                                 acCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatggcat                                                                                    >  2:217086/1‑149 (MQ=255)                                                                     cccTTTCACGGCGGTAACAGGGGTTCGAGGCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAAGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCag                                                                                <  7:173252/149‑1 (MQ=255)                                                                          tCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCaggag                                                                             <  2:114503/147‑1 (MQ=255)                                                                            aCGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGGCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCa                                                                         <  1:366905/149‑1 (MQ=255)                                                                                   tAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTc                                                                  <  1:181279/149‑1 (MQ=255)                                                                                      cAGGGTTTCTAGTCCCCTATGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTAAAAAACGCTCGTCGATTATTGATGATCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGAt                                                               <  4:236954/149‑1 (MQ=37)                                                                                          ggTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCg                                                           <  7:188610/149‑1 (MQ=255)                                                                                          ggTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCg                                                           <  3:221268/149‑1 (MQ=255)                                                                                            ttCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCt                                                         >  2:371426/1‑149 (MQ=255)                                                                                                    cccTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCACAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCTCTTAGGTcca                                                 >  6:348637/1‑149 (MQ=255)                                                                                                     ccTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccac                                                >  1:3695/1‑149 (MQ=255)                                                                                                      cTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccac                                                >  3:48066/1‑148 (MQ=255)                                                                                                      cTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccac                                                >  8:168442/1‑148 (MQ=255)                                                                                                          gggACACCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAtt                                            >  6:333677/1‑148 (MQ=255)                                                                                                               gCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGCTCCCGCTTAGCTCCACCATTTCTGcc                                      <  1:217086/149‑1 (MQ=255)                                                                                                               gCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGc                                       >  5:323236/1‑148 (MQ=255)                                                                                                                 cACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAg                                    <  6:47909/149‑1 (MQ=255)                                                                                                                     tttCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGt                                 >  5:373750/1‑148 (MQ=255)                                                                                                                          tGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACg                            >  3:112462/1‑148 (MQ=255)                                                                                                                                   cAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGAt                   <  1:371426/148‑1 (MQ=255)                                                                                                                                       gaaacaaaaGGCTCATCAATAATCGACGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTcg              >  6:419575/6‑149 (MQ=21)                                                                                                                                       gCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTcg              <  4:112462/149‑1 (MQ=255)                                                                                                                                         ttAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCt                                                         >  3:224902/1‑104 (MQ=255)                                                                                                                                         ttAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCt                                                         <  4:224902/104‑1 (MQ=255)                                                                                                                                            aaaaGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAg         <  1:86079/149‑1 (MQ=255)                                                                                                                                            aaaaGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAg         <  6:373750/149‑1 (MQ=255)                                                                                                                                            aaaaGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAg         <  6:323236/149‑1 (MQ=255)                                                                                                                                             aaaGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAgg        >  5:72489/1‑149 (MQ=255)                                                                                                                                               aGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGtt      <  3:38563/149‑1 (MQ=255)                                                                                                                                                   tcgtcgATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGt  >  1:62325/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGT  >  NC_002947/2241889‑2242183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑