| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,254,524 | C→A | 5.1% | H281Q (CAC→CAA) | leuB → | 3‑isopropylmalate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,254,524 | 0 | C | A | 5.1% | 256.4 / 3.2 | 79 | H281Q (CAC→CAA) | leuB | 3‑isopropylmalate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (40/35); new base A (2/2); total (42/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.74e-01 | |||||||||||
CCCAAACAGTTCGACGTGATGGTGACCGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGCCTGGTTCTGGACCAGGGG > NC_002947/2254381‑2254671 | cccAAACAGTTCGACGTGATGGTGACCGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTc > 2:199112/1‑149 (MQ=255) aCAGTTCGACGTGATGGTGACCGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCGTGGCAAGGCTCgtaca < 8:347628/149‑5 (MQ=255) aGTTCGACGAGATGGTGACCGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGATCCTTTCCACGGCTCGGCACCg > 5:233028/1‑149 (MQ=255) ttCGACGTGATGGTGACCGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGa > 5:407961/1‑149 (MQ=255) cGACGTGATGGTGACCGCCAACATGTTCGGGGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATTCTGCCTTCGGCGTCCCTGGATGCGGACAACATCGGCATGTACGAGCCTTGCCACGGCTGGGCACCGGAc < 6:233028/148‑1 (MQ=255) aCGTGATGGTGACCGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCGTGGCAAGGCTCgtaca > 6:72549/1‑137 (MQ=255) aCGTGATGGTGACCGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCGTGGCAAGGCTCgtaca < 5:72549/141‑5 (MQ=255) tGGTGACCGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGc < 3:186494/149‑1 (MQ=255) tGGTGACCGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGc > 4:161364/1‑149 (MQ=255) ggTGACCGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCCGCATCCTGCCTACCGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGcc > 2:107373/1‑149 (MQ=255) tGACCGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCtt > 7:340909/1‑149 (MQ=255) tGACCGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCtt > 3:334065/1‑149 (MQ=255) aCCGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTg < 6:407961/148‑1 (MQ=255) cGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCa > 1:118785/1‑149 (MQ=255) caacaTGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGTCCTTGGCATCg > 8:158248/1‑149 (MQ=255) caacaTGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCg < 5:196575/149‑1 (MQ=255) caacaTGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCg < 4:389550/149‑1 (MQ=255) aTGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCaa > 4:107375/1‑149 (MQ=255) tGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTg < 2:163419/140‑1 (MQ=255) tGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTg > 1:163419/1‑140 (MQ=255) gATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTc > 4:122104/1‑149 (MQ=255) gATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATAGCCAACCCACTGGCGACCATCCTGTc < 1:216743/149‑1 (MQ=255) gAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGgt > 1:280738/1‑149 (MQ=255) aGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGgtgt > 8:365857/1‑149 (MQ=255) tGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGatgatg > 2:199559/1‑149 (MQ=255) gACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGc > 5:206651/1‑149 (MQ=255) gACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGc > 4:363362/1‑149 (MQ=255) gACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGc > 3:66536/1‑149 (MQ=255) aCCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGc < 1:249994/148‑1 (MQ=255) aCCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGc < 3:363362/148‑1 (MQ=255) aCCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGAGGTTGCg > 3:321772/1‑149 (MQ=255) cGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGt > 4:42160/1‑148 (MQ=255) cGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGACTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGt > 8:27476/1‑148 (MQ=255) ttCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACa < 7:367038/149‑1 (MQ=255) aTCGGCATGCTGCCTTCGGCGTCGCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCtt < 2:210307/149‑1 (MQ=255) aTCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCtt > 1:189506/1‑149 (MQ=255) ggCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCaa < 8:340909/149‑1 (MQ=255) ggCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCaa < 3:161364/149‑1 (MQ=255) gctgcCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGt > 1:174909/1‑149 (MQ=255) ctgcCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGtt < 7:242350/128‑1 (MQ=255) ctgcCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGtt > 8:242350/1‑128 (MQ=255) ctgcCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGt < 7:27476/148‑1 (MQ=255) ctgcCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTc < 3:122104/149‑1 (MQ=255) tgcCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCg > 5:334341/1‑149 (MQ=255) gcCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCgg < 4:139801/149‑1 (MQ=255) cTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCt < 1:107373/149‑1 (MQ=255) ttCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTg > 5:74159/1‑149 (MQ=255) ttCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTg > 4:170357/1‑149 (MQ=255) ttCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTg > 2:311871/1‑149 (MQ=255) tCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGa < 2:97328/149‑1 (MQ=255) ccTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAgg < 6:334341/149‑1 (MQ=255) gATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGa < 1:311871/148‑1 (MQ=255) gATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGa < 1:199559/148‑1 (MQ=255) ggCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCg < 8:322705/148‑1 (MQ=255) ccGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCga < 8:233561/148‑1 (MQ=255) cGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCgag > 3:386467/1‑148 (MQ=255) gtggcaaGGCTCGTACATGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAgg > 7:143445/6‑149 (MQ=255) aacaaAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGc > 3:79957/1‑149 (MQ=255) caaAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATc > 4:53953/1‑139 (MQ=255) caaAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATc < 3:53953/139‑1 (MQ=255) aaaGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGt < 3:170357/149‑1 (MQ=255) aaGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTg < 2:174909/149‑1 (MQ=255) gCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGc > 1:262551/1‑149 (MQ=255) cATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGcc < 7:365857/149‑1 (MQ=255) aTGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGcc > 6:7905/1‑141 (MQ=255) aTGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGcc < 5:7905/141‑1 (MQ=255) aTGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGCCt < 7:158248/149‑1 (MQ=255) aCGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGcc < 2:96953/137‑1 (MQ=255) aCGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGcc > 1:96953/1‑137 (MQ=255) aCGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGCCTGGtt > 2:232554/1‑149 (MQ=255) cGAGCCGTGGCAAGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGCCTGGTTc > 3:397531/1‑149 (MQ=255) gAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGCCTGGTTCt > 6:205428/1‑149 (MQ=255) ccTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGCCTGGTTCTGGa > 5:335284/1‑149 (MQ=255) ccTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGCCTGGTTCTGGa > 3:289767/1‑149 (MQ=255) ttGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGCCTGGTTCTGGAc > 5:102578/1‑148 (MQ=255) gCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGCCTGGTTCTGGACCa < 6:74159/148‑1 (MQ=255) ccACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGCCTGGTTCTGGACCAgg < 3:42160/149‑1 (MQ=255) ccACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGCCTGGTTCTGGACCAgg < 3:302370/149‑1 (MQ=255) aCGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGCCTGGTTCTGGACCAgggg < 2:280738/149‑1 (MQ=255) | CCCAAACAGTTCGACGTGATGGTGACCGACAACATGTTCGGTGACATTCTGTCGGATGAAGCTTCCATGTTGACCGGTTCCATCGGCATGCTGCCTTCGGCGTCCCTGGATGCCGACAACAAAGGCATGTACGAGCCTTGCCACGGCTCGGCACCGGACATCGCCGGCCTTGGCATCGCCAACCCACTGGCGACCATCCTGTCGGTGTCGATGATGTTGCGTTACAGCTTCAATCAGTCGGCTGCCGCCGAGGCGATCGAGAAGGCCGTGAGCCTGGTTCTGGACCAGGGG > NC_002947/2254381‑2254671 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |