Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,264,563 G→A 100% M171I (ATG→ATA)  accD → acetyl‑CoA carboxylase carboxyltransferase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,264,5630GA98.4% 195.7 / ‑4.6 62M171I (ATG→ATA) accDacetyl‑CoA carboxylase carboxyltransferase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (27/33);  total (27/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATGCAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTGACCGACCCGGTGTATGGCGGTGTTTCCGCCAGCCTGGCGATGCTCGGCGAC  >  NC_002947/2264417‑2264707
                                                                                                                                                   |                                                                                                                                                
ccGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACa                                                                                                                                                <  2:76439/149‑1 (MQ=255)
       tGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAgcg                                                                                                                                         <  6:274643/149‑1 (MQ=255)
       tGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAgcg                                                                                                                                         >  3:161653/1‑149 (MQ=255)
         gTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCt                                                                                                                                       <  6:34040/149‑1 (MQ=255)
            aGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGa                                                                                                                                     <  7:33412/148‑1 (MQ=255)
                ccTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCg                                                                                                                                <  1:389080/149‑1 (MQ=255)
                 cTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGc                                                                                                                               <  8:57076/149‑1 (MQ=255)
                          ctctTTCATGGGCTGCTCCATGTGTGCCATCGTCGGAGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGTCGGTGCGCGCATACAGGAAGCTCTGATCTCGCTGATGCAg                                                                                                                       <  4:294013/148‑1 (MQ=255)
                           tcGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGGTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGAt                                                                                                                     >  1:127350/1‑149 (MQ=255)
                           tcGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGAt                                                                                                                     >  5:194646/1‑149 (MQ=255)
                              ttCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGc                                                                                                                  >  5:111316/1‑149 (MQ=255)
                                   gggCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGa                                                                                                             >  6:221913/1‑149 (MQ=255)
                                   gggCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGa                                                                                                             >  3:71767/1‑149 (MQ=255)
                                    ggcgtcTCCATGGGTGCCAACGTCGGCGAGCGCCTCGTCCGGGCGGCCAACAATGCCCTGGAAAACCGCTGCCCAATGGCTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATTCAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGa                                                                                                             <  2:318750/148‑1 (MQ=255)
                                    ggcggcTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGAc                                                                                                            <  2:32644/149‑1 (MQ=255)
                                        gcTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACctct                                                                                                        <  6:194646/149‑1 (MQ=255)
                                          tCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGACCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCGTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTg                                                                                                       >  4:21354/1‑148 (MQ=255)
                                            cATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTg                                                                                                       <  3:21354/146‑1 (MQ=255)
                                               gggTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGc                                                                                                 >  4:162762/1‑149 (MQ=255)
                                                  tGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTgg                                                                                              <  3:198475/149‑1 (MQ=255)
                                                     cATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGgcgc                                                                                           >  3:201967/1‑149 (MQ=255)
                                                         gtcgGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCt                                                                                       <  6:111316/149‑1 (MQ=255)
                                                               gAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATGCAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCga                                                                                 <  4:166894/149‑1 (MQ=255)
                                                               gAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGcgcg                                                                                  >  8:283822/1‑148 (MQ=255)
                                                                aGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGcgcg                                                                                  >  4:71162/1‑147 (MQ=255)
                                                                  cgcTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCgaaga                                                                              <  8:97627/149‑1 (MQ=255)
                                                                   gcTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCgaagaa                                                                             <  1:60317/149‑1 (MQ=255)
                                                                    cTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGaagaag                                                                            <  7:283822/149‑1 (MQ=255)
                                                                     ttCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAgg                                                                           >  4:220722/1‑149 (MQ=255)
                                                                     ttCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAgg                                                                           >  4:377653/1‑149 (MQ=255)
                                                                       cGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAgg                                                                           <  4:201967/147‑1 (MQ=255)
                                                                       cGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGc                                                                          <  4:161653/148‑1 (MQ=255)
                                                                       cGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCAGGcgcgccagcac                                                                           <  3:162762/147‑8 (MQ=255)
                                                                        gTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCAt                                                                        <  3:377653/149‑1 (MQ=255)
                                                                         tCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATc                                                                       >  6:320348/1‑149 (MQ=255)
                                                                         tCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATc                                                                       >  8:134364/1‑149 (MQ=255)
                                                                          ccGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATcc                                                                      <  1:374500/149‑1 (MQ=255)
                                                                           cGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATccc                                                                     >  3:343380/1‑149 (MQ=255)
                                                                            gTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCg                                                                    >  5:30415/1‑149 (MQ=255)
                                                                            gTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCg                                                                    <  3:220722/149‑1 (MQ=255)
                                                                            gTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCg                                                                    >  4:298243/1‑149 (MQ=255)
                                                                                     aCTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCg                                                           <  7:134364/149‑1 (MQ=255)
                                                                                     aCTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCg                                                           <  3:298243/149‑1 (MQ=255)
                                                                                           cccTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACt                                                      <  3:368801/148‑1 (MQ=255)
                                                                                            ccTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTga                                                    >  3:362120/1‑149 (MQ=255)
                                                                                             cTGGAAAACCGCTGCCCAATGGTTTGCTTGCTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTga                                                    >  1:30414/1‑149 (MQ=255)
                                                                                                gAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTgaccga                                                >  4:345512/1‑149 (MQ=255)
                                                                                                gAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTgacc                                                  <  5:221913/147‑1 (MQ=255)
                                                                                                      cGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTGACCGACCCGgt                                          <  3:345512/149‑1 (MQ=255)
                                                                                                           cccAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTgc                                                                                     >  5:99882/1‑101 (MQ=255)
                                                                                                           cccAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTgc                                                                                     <  6:99882/101‑1 (MQ=255)
                                                                                                           cccAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTGACCGACCCGGTGTATg                                     <  3:71162/149‑1 (MQ=255)
                                                                                                            ccAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTGACCGACCCGGTGTATgg                                    >  6:269830/1‑149 (MQ=255)
                                                                                                            ccAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTGACCGACCCGGTGTATgg                                    <  5:373760/149‑1 (MQ=255)
                                                                                                                tGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTGACCGACCCGGTGTATGGCGgt                                <  3:126330/149‑1 (MQ=255)
                                                                                                                  gTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTGACCGACCCGGTGTATGGCGgtgt                              >  7:297250/1‑149 (MQ=255)
                                                                                                                   tttGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTgac                                                   <  6:239407/127‑1 (MQ=255)
                                                                                                                   tttGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTgac                                                   >  5:239407/1‑127 (MQ=255)
                                                                                                                   tttGCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTGACCGACCCGGTGTATGGCGGTGtt                             <  6:372019/149‑1 (MQ=255)
                                                                                                                      gCTT‑CTCCGCTTCGGGCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTGACCGACCCGGTGTATGGCGGTGTTTcc                          >  4:306631/1‑149 (MQ=255)
                                                                                                                                     ggCGGTGCGCGCATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTGACCGACCCGGTGTATGGCGGTGTTTCCGCCAGCCTGGCGAt            >  5:13369/1‑149 (MQ=255)
                                                                                                                                               gcATACAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTTCTGACCGACCCGGTGTATGGCGGTGTTTCCGCCAGCCTGGCGATGCTCGGCGAc  <  8:297250/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCGATCGTGGTTAGCGCCTTCGAGTTCTCGTTCATGGGCGGCTCCATGGGTGCCATCGTCGGCGAGCGCTTCGTCCGTGCGGCCAACTATGCCCTGGAAAACCGCTGCCCAATGGTTTGCTT‑CTCCGCTTCGGGCGGTGCGCGCATGCAGGAAGCGCTGATCTCGCTGATGCAGATGGCCAAGACCTCTGCCGTGCTGGCGCGCCTGCGCGAAGAAGGCATCCCGTTCATCTCGGTACTGACCGACCCGGTGTATGGCGGTGTTTCCGCCAGCCTGGCGATGCTCGGCGAC  >  NC_002947/2264417‑2264707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑