BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,298,604	G→A	5.1%	D506D (GAC→GAT)	sbcC ←	exonuclease subunit SbcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,298,604	0	G	A	5.1%	303.7 / 2.8	97	D506D (GAC→GAT)	sbcC	exonuclease subunit SbcC
Reads supporting (aligned to +/- strand): ref base G (43/49); new base A (2/3); total (45/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 102 total aligned reads displayed.

TTGCTGGCGTTGCAGCATGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGTCAGCCGGC  >  NC_002947/2298457‑2298752

ttGCTGGCGTTGCAGCATGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGt                                                                                                                                                      <  6:131121/149‑1 (MQ=255)

  gCTGGCGTTGCAGCATGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTc                                                                                                                                                     >  8:326987/1‑148 (MQ=255)

    tGGCGTTGCAGCATGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGAt                                                                                                                                                  >  1:202238/1‑149 (MQ=255)

    tGGCGTTGCAGCATGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGAt                                                                                                                                                  >  1:366394/1‑149 (MQ=255)

     ggCGTTGCAGCATGGCCGGCTGCTGCCGTGCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATGTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGATCGacc                                                                                                                                                 <  5:40843/149‑3 (MQ=255)

         ttGCAGCATGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCt                                                                                                                                             >  1:296803/1‑149 (MQ=255)

              gcaTGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGATCGACCTGCTCGGcg                                                                                                                                        <  7:216821/149‑2 (MQ=255)

              gcaTGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGATCGACCTGCTCGGcg                                                                                                                                        <  6:184037/149‑2 (MQ=255)

                aTGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑g                                                                                                                                     <  1:392539/149‑1 (MQ=255)

                    ccTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGcc                                                                                                                                 >  6:369213/1‑149 (MQ=255)

                        ctgctgcCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTggg                                                                                                                             <  1:391891/149‑1 (MQ=255)

                        ctgctgcCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTgg                                                                                                                              <  1:208258/148‑1 (MQ=255)

                            tgcCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTc                                                                                                                         >  3:369134/1‑149 (MQ=255)

                             gcCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCg                                                                                                                        >  6:43561/1‑149 (MQ=255)

                              ccGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCgg                                                                                                                       >  6:304569/1‑149 (MQ=255)

                              ccGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCg                                                                                                                        <  5:104304/148‑1 (MQ=255)

                                gTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGcc                                                                                                                     <  7:326987/149‑1 (MQ=255)

                                gTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGcc                                                                                                                     >  5:230373/1‑149 (MQ=255)

                                    gcgcAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTgg                                                                                                                 <  2:296803/149‑1 (MQ=255)

                                    gcgcAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTg                                                                                                                  <  5:304569/148‑1 (MQ=255)

                                     cgcAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGc                                                                                                                <  2:202238/149‑1 (MQ=255)

                                         gggCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGTAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCa                                                                                                            <  7:163382/149‑1 (MQ=255)

                                             aTCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACgg                                                                                                         >  3:339559/1‑148 (MQ=255)

                                              tCCAGCTGCTGTCGCAGCTCCTGTTCTCGGGCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTTTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGaa                                                                                                       <  6:195664/149‑1 (MQ=255)

                                                  gctgctGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGaacaa                                                                                                    >  2:267596/1‑148 (MQ=255)

                                                   ctgctgGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAacaaca                                                                                                  <  8:358290/149‑1 (MQ=255)

                                                    tgctgGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAg                                                                                                 >  7:250222/1‑149 (MQ=255)

                                                     gctgGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGc                                                                                                                      >  5:92916/1‑127 (MQ=255)

                                                     gctgGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGc                                                                                                                      <  6:92916/127‑1 (MQ=255)

                                                         gcgcAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCg                                                                                             >  4:219518/1‑148 (MQ=255)

                                                           gcAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAg                                                                                          >  6:128847/1‑149 (MQ=255)

                                                           gcAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAg                                                                                          <  4:36920/149‑1 (MQ=255)

                                                            cAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTc                                                                                                                                            <  6:46605/99‑1 (MQ=255)

                                                            cAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTc                                                                                                                                            >  5:46605/1‑99 (MQ=255)

                                                               cTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGt                                                                                      <  7:175951/149‑1 (MQ=255)

                                                                 ccTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTcg                                                                                    >  3:247496/1‑149 (MQ=255)

                                                                     ttCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCt                                                                                >  3:53765/1‑149 (MQ=255)

                                                                        aCGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGG                                                                             >  3:373208‑M1/1‑149 (MQ=255)

                                                                        aCGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCG                                                                              >  4:178479‑M1/1‑148 (MQ=255)

                                                                        aCGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCG                                                                              <  3:178479‑M1/148‑1 (MQ=255)

                                                                         cGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGC                                                                            <  4:53765‑M1/149‑1 (MQ=255)

                                                                          ggCCGTGCAGGCGTGACATTTCCTCTACTGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCC                                                                           <  4:369134‑M1/149‑1 (MQ=255)

                                                                          ggCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCC                                                                           >  7:86257‑M1/1‑149 (MQ=255)

                                                                          ggCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCC                                                                           >  1:358881‑M1/1‑149 (MQ=255)

                                                                            ccGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTG                                                                         >  8:306070‑M1/1‑149 (MQ=255)

                                                                              gTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGC                                                                        >  5:133839‑M1/1‑148 (MQ=255)

                                                                               tGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCaa                                                                      <  6:133839/149‑1 (MQ=255)

                                                                                gCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAg                                                                     >  7:210947/1‑149 (MQ=255)

                                                                                    gCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAgcgcg                                                                 >  1:250781/1‑149 (MQ=255)

                                                                                      gTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGC                                                                        >  6:394125‑M1/1‑140 (MQ=255)

                                                                                      gTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGC                                                                        <  5:394125‑M1/140‑1 (MQ=255)

                                                                                       tGACA‑TTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCAtt                                                             >  4:296064/1‑149 (MQ=255)

                                                                                         aCATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCAtt                                                             <  5:369213/148‑1 (MQ=255)

                                                                                         aCATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTg                                                            <  5:43561/149‑1 (MQ=255)

                                                                                           aTTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGc                                                          <  7:304886/149‑1 (MQ=255)

                                                                                            tttCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGc                                                          >  8:358096/1‑148 (MQ=255)

                                                                                            tttCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGc                                                          <  7:358096/148‑1 (MQ=255)

                                                                                                ctctACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCctggc                                                     <  2:250781/149‑1 (MQ=255)

                                                                                                 tctACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCctggct                                                    >  2:273338/1‑149 (MQ=255)

                                                                                                     cGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCctggctgg                                                  <  8:250222/147‑1 (MQ=255)

                                                                                                       gcgcgcTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTggcctg                                              >  2:327785/1‑149 (MQ=255)

                                                                                                        cgcgcTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGGCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTggcctgg                                             <  7:306070/149‑1 (MQ=255)

                                                                                                          cgcTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTggcctgggc                                           <  4:339559/149‑1 (MQ=255)

                                                                                                             tgctgTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCtg                                        <  5:323644‑M2/149‑3 (MQ=255)

                                                                                                              gctgTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCtg                                        <  7:348274‑M2/148‑3 (MQ=255)

                                                                                                                tgTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCtgcag                                     >  8:210947‑M2/1‑144 (MQ=255)

                                                                                                                  tttGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAggcccaggccagccaggccaatgcgcgcttgcag                                     <  2:365404‑M1/147‑35 (MQ=255)

                                                                                                                     gCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCtg                                        <  5:99622‑M2/141‑3 (MQ=255)

                                                                                                                     gCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCtg                                        >  6:99622‑M2/1‑139 (MQ=255)

                                                                                                                     gCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCgg                                >  6:330563/1‑149 (MQ=255)

                                                                                                                      cGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCggg                               <  4:373208/149‑1 (MQ=255)

                                                                                                                       ggTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCggg                               >  7:185317/1‑148 (MQ=255)

                                                                                                                       ggTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGt                              <  3:219518/149‑1 (MQ=255)

                                                                                                                        gTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCggg                               >  1:337019/1‑147 (MQ=255)

                                                                                                                          tGTACTGCAACATCCCGCCGAGCAGGTCTATCTGCTCGGCCA‑TGGCCTGGGGTTCTGCCTTGTCTGCAAGGAACAACTTGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTTGCACGTCTGGCCTGGTCCTGAAGGCCGTGTat                            <  6:230373/148‑2 (MQ=255)

                                                                                                                               tGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCtcttc                      >  7:379983/1‑149 (MQ=255)

                                                                                                                                gCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCtcttct                     >  5:10508/1‑149 (MQ=255)

                                                                                                                                gCAACATGCCGCCGAGCAGGTCGATCTGCTAGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCtcttct                     >  1:241195/1‑149 (MQ=255)

                                                                                                                                 cAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCtcttct                     <  5:330563/148‑1 (MQ=255)

                                                                                                                                   aCATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCgg                  <  2:358881/149‑1 (MQ=255)

                                                                                                                                     aTGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGcc                <  4:247496/149‑1 (MQ=255)

                                                                                                                                       gccgccGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGGCTGCAGGTCGGGTAGCtcttc                      <  2:21875/141‑1 (MQ=255)

                                                                                                                                       gccgccGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGTCGGGTAGCtcttc                      >  1:21875/1‑141 (MQ=255)

                                                                                                                                       gccgccGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCt              >  1:406527/1‑149 (MQ=255)

                                                                                                                                         cgccgAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTg            <  1:267596/149‑1 (MQ=255)

                                                                                                                                         cgccgAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTg            <  1:327785/149‑1 (MQ=255)

                                                                                                                                         cgccgAGCAGGTCGATCTGCCCGGCCATGGGCCTGGGGTTCGGCCTTGGCTTCAAGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCtt             >  1:17624/1‑149 (MQ=255)

                                                                                                                                           ccgAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGt          <  6:323807/149‑1 (MQ=255)

                                                                                                                                           ccgAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGt          <  8:348274/149‑1 (MQ=255)

                                                                                                                                           ccgAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGt          <  8:185317/149‑1 (MQ=255)

                                                                                                                                            cgAGCAGATCGACCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGt          >  5:83139/1‑148 (MQ=255)

                                                                                                                                            cgAGCAGATCGACCTGCTCGGCCA‑GGGCCTGCGGTTCGGCCTTGGCGTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCAGGGCCTGCCGGCCGGGTAGCTCTTATCGGCCCTTGGt          >  5:21536/1‑148 (MQ=255)

                                                                                                                                             gAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGTCa        <  3:296064/149‑1 (MQ=255)

                                                                                                                                               gCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGTCAGc      <  6:10508/149‑1 (MQ=255)

                                                                                                                                                cAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGTCAGcc     <  1:273338/149‑1 (MQ=255)

                                                                                                                                                  ggTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGTCAGCCgg   >  8:38178/1‑149 (MQ=255)

                                                                                                                                                   gTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGt                              >  7:382626/1‑121 (MQ=255)

                                                                                                                                                   gTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGt                              <  8:382626/121‑1 (MQ=255)

                                                                                                                                                   gTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGTCAGCCGGc  >  5:220389/1‑149 (MQ=255)

                                                                                                                                                   gTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGTCAGCCGGc  >  7:17673/1‑149 (MQ=255)

TTGCTGGCGTTGCAGCATGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCA‑GGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGTCAGCCGGC  >  NC_002947/2298457‑2298752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`