Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,335,393 A→T 5.8% F355Y (TTC→TAC)  PP_2052 ← bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,335,3930AT 5.8% 212.2 / 2.2 69F355Y (TTC→TAC) PP_2052bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (35/30);  new base T (2/2);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

ATGGGTCTTCTGCAAAACCTGCTCGCTGATCGCAGGCGGGCACAGTAGCGACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGAT  >  NC_002947/2335251‑2335538
                                                                                                                                              |                                                                                                                                                 
aTGGGTCTTCTGCAAAACCTGCTCGCTGATCGCAGGCGGGCACAGTAGCGACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGtt                                                                                                                                             <  8:165376/149‑1 (MQ=255)
aTGGGTCTTCTGCAAAACCTGCTCGCTGATCGCAGGCGGGCACAGTAGCGACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGtt                                                                                                                                             <  4:77678/149‑1 (MQ=255)
 tGGGACTTCTGCAAAACCTGCTCGCTGATCGCAGGCGGGCAGAGTAGCGACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGCCCTCGGCGCTCGAAgacttg                                                                                                                                            >  2:280630/1‑144 (MQ=255)
    gTCTTCTGCAAAACCTGCTCGCTGATCGCAGGCGGGCACAGTAGCGACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTgcag                                                                                                                                         <  3:106425/149‑1 (MQ=255)
       ttctGCAAAACCTGCTCGCTGATCGCAGGCGGGCACAGTAGCGACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGcagcag                                                                                                                                      >  6:53529/1‑149 (MQ=255)
                    gCTCGCTGATCGCAGGCGGGCACAGTAGCGACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGAt                                                                                                                          >  2:34170/1‑148 (MQ=255)
                     cctgctgatcgtgctgaacaaggttggcggtgcagcgttcgtgcggcgtcatgtgcaagccgagttgtcgctactgtgcccgcctgcgatcagcAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCa                                                                                                                        >  7:346361‑M2/95‑149 (MQ=255)
                       tgctgatcgtgctgaacaaggttggcggtgcagcgttcgtgcggcgtcatgtgcaagccgagttgtcgctactgtgcccgcctgcgatcagcAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCAcc                                                                                                                      >  5:143050‑M2/93‑149 (MQ=255)
                          TGATCGCAGGCGGGCACAGTAGCGACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACcctg                                                                                                                   <  1:365260‑M1/149‑1 (MQ=255)
                            ATCGCAGGCGGGCACAGTAGCGACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACcctggc                                                                                                                 >  7:252791‑M1/1‑149 (MQ=255)
                            ATCGCAGGCGGGCACAGTAGCGACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACcctgg                                                                                                                  >  3:162904‑M1/1‑148 (MQ=255)
                                  ctgaacaaggttggcggtgcagcgttcgtgcggcgtcatgtgcaagccgagttgtcgctactgtgcccgcctgcgatcagcAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCctggcctggc                                                                                                            <  8:346361‑M2/67‑1 (MQ=255)
                                                  aCAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTc                                                                                                         >  6:251145/1‑135 (MQ=255)
                                                  aCAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTc                                                                                                         <  5:251145/135‑1 (MQ=255)
                                                  aCAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCg                                                                                           >  4:151471/1‑149 (MQ=255)
                                                     aCTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGc                                                                                        <  6:172164/149‑1 (MQ=255)
                                                      cTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCggg                                                                                         >  5:55617/1‑147 (MQ=255)
                                                       tCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAg                                                                                      >  2:404940/1‑149 (MQ=255)
                                                         ggCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGc                                                                                    >  7:86616/1‑149 (MQ=255)
                                                          gCTTGTACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCt                                                                                   >  2:236085/1‑149 (MQ=255)
                                                          gCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCt                                                                                   >  8:199516/1‑149 (MQ=255)
                                                              gCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCa                                                                                 <  3:367793/147‑1 (MQ=255)
                                                              gCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAgg                                                                               >  5:136563/1‑149 (MQ=255)
                                                              gCACATGACGCCGCACGAACGCTGCACCACCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCa                                                                                 <  8:15368/147‑1 (MQ=255)
                                                                  aTGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCga                                                                           >  1:192246/1‑149 (MQ=255)
                                                                  aTGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCga                                                                           <  3:151471/149‑1 (MQ=255)
                                                                  aTGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCga                                                                           <  4:134959/149‑1 (MQ=255)
                                                                      cgccgcACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCAGCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCgatgat                                                                       <  6:55617/149‑1 (MQ=255)
                                                                       gccgcACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCTGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCa                                                                                 <  1:308966/138‑1 (MQ=255)
                                                                       gccgcACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCa                                                                                 >  2:308966/1‑138 (MQ=255)
                                                                       gccgcACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGAtt                                                                      >  4:24365/1‑149 (MQ=255)
                                                                         cgcACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTc                                                                    <  1:406840/149‑1 (MQ=255)
                                                                          gcACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGTAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCg                                                                   >  2:370397/1‑149 (MQ=255)
                                                                          gcACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGTAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCg                                                                   >  2:365390/1‑149 (MQ=255)
                                                                          gcACGAACGCTGCACCGCCAACCTTCTTCACCACGAGCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCg                                                                   <  1:280630/149‑1 (MQ=255)
                                                                           cACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCgg                                                                  <  1:236085/149‑1 (MQ=255)
                                                                            aCGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTc                                                                    >  5:94760/1‑146 (MQ=255)
                                                                            aCGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTc                                                                    <  6:94760/146‑1 (MQ=255)
                                                                             cGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGGTCGGGCAGGCTCAGGCCGATGATTTCGGCa                                                                <  1:34170/149‑1 (MQ=255)
                                                                                  gCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAgtggt                                                           <  2:192246/149‑1 (MQ=255)
                                                                                         cGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATg                                                    >  5:238040/1‑149 (MQ=255)
                                                                                          gctactgtgcccgcctgcgatcagcAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGc                                                   <  6:143050‑M2/124‑1 (MQ=255)
                                                                                          gCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGc                                                   <  1:404940/149‑1 (MQ=255)
                                                                                           ccAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACaaca                                                   >  8:211830/1‑145 (MQ=255)
                                                                                             aaCCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGTAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCtga                                                <  1:365390/149‑1 (MQ=255)
                                                                                             aaCCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGTAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCtga                                                <  1:370397/149‑1 (MQ=255)
                                                                                               ccTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCtgat                                               >  2:233650/1‑148 (MQ=255)
                                                                                                cTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCtt                                                                                                          <  6:331381/88‑1 (MQ=255)
                                                                                                cTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCtt                                                                                                          >  5:331381/1‑88 (MQ=255)
                                                                                                    ttCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACcgc                                         >  8:326804/1‑149 (MQ=255)
                                                                                                      cAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGgtgt                                                         <  2:209989/131‑1 (MQ=255)
                                                                                                      cAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGgtgt                                                         >  1:209989/1‑131 (MQ=255)
                                                                                                      cAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGcgt                                       >  2:10302/1‑149 (MQ=255)
                                                                                                       aGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGcgtc                                      <  6:136563/149‑1 (MQ=255)
                                                                                                       aGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGcgtc                                      >  7:241520/1‑149 (MQ=255)
                                                                                                          aCGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGcgtcgtc                                   <  3:24365/149‑1 (MQ=255)
                                                                                                           cgatcagcAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGcgtcgtcg                                  <  5:53529‑M2/141‑1 (MQ=255)
                                                                                                            gatcagcAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGcgtcgtcgt                                 >  7:10539‑M2/8‑149 (MQ=255)
                                                                                                            gatcagcAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGcgtcgtcgt                                 >  5:62832‑M2/8‑149 (MQ=255)
                                                                                                                  cAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGTGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCa                           >  1:340062‑M2/2‑149 (MQ=255)
                                                                                                                     gggcAGCGCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGt                        <  1:381229/146‑1 (MQ=255)
                                                                                                                         aGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGAt                    >  5:133611/1‑149 (MQ=255)
                                                                                                                            tCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACcgcg                                        >  7:147251/1‑126 (MQ=255)
                                                                                                                            tCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACcgcg                                        <  8:147251/126‑1 (MQ=255)
                                                                                                                            tCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCAt                 >  4:361267/1‑149 (MQ=255)
                                                                                                                                 gCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCa            >  6:210086/1‑149 (MQ=255)
                                                                                                                                   cTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACg          >  4:112313/1‑149 (MQ=255)
                                                                                                                                        cgcTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTc     <  6:238040/149‑1 (MQ=255)
                                                                                                                                             gAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGAt  <  5:296209/147‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
ATGGGTCTTCTGCAAAACCTGCTCGCTGATCGCAGGCGGGCACAGTAGCGACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGAT  >  NC_002947/2335251‑2335538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑