Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,336,497 C→T 13.6% intergenic (‑41/+39) PP_2052 ← / ← PP_5500 bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,336,4970CT13.6% 107.9 / 8.6 44intergenic (‑41/+39)PP_2052/PP_5500bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (17/21);  new base T (3/3);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CAGAGAAGCTTGCTTGAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAGG  >  NC_002947/2336349‑2336641
                                                                                                                                                     |                                                                                                                                                
cAGAGAAGCTTGCTTGAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGc                                                                                                                                                  <  4:398773/149‑1 (MQ=255)
        cttgcttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCg                                                                                                                                          <  3:283168/149‑1 (MQ=255)
            cttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgaga                                                                                                                                       <  5:232862/148‑1 (MQ=255)
               gcctgtCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACgg                                                                                                                                   <  5:167362/146‑1 (MQ=255)
               gAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACg                                                                                                                                    <  1:21160/148‑1 (MQ=255)
                aGTGTCCTGAAGCGCAGGCGCTCGGTGTCGACGAGGGTGCCATCCTAATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgt                                                                                                                                  <  4:294103/149‑1 (MQ=255)
                  tgtCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgt                                                                                                                                <  5:133792/149‑1 (MQ=255)
                    tCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCTTCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgtgt                                                                                                                              >  2:93318/1‑149 (MQ=255)
                    tCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgtgt                                                                                                                              <  4:362559/149‑1 (MQ=255)
                    tCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgtgt                                                                                                                              <  3:315084/149‑1 (MQ=255)
                      cTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgtgt                                                                                                                              >  1:146586/1‑147 (MQ=255)
                       tGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgagat                                                                                                                                      <  1:61271/138‑2 (MQ=255)
                       tGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgagat                                                                                                                                      <  1:67139/138‑2 (MQ=255)
                       tGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgagat                                                                                                                                      >  2:67139/1‑137 (MQ=255)
                       tGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgagat                                                                                                                                      >  2:61271/1‑137 (MQ=255)
                         aaGCGCTGGCGATCGGTGTCGACGTGGGTGCCAACCATATCGAAGATGGCGCTGAAAATCTGTTTACGGTGAAAGAAAATCTTTTGGTTTTGCCCGTTTT‑CGTTGCTTATGGCATCTGGCGTTCAACTTGGGTTAACGGTGTGTGAg                                                                                                                           <  4:67901/147‑1 (MQ=255)
                               aGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTg                                                                                                                   >  2:58862/1‑149 (MQ=255)
                                ggCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTgg                                                                                                                  <  5:247836/149‑1 (MQ=255)
                                  cgcTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTgggg                                                                                                                <  5:313372/149‑1 (MQ=255)
                                  cgcTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTgggg                                                                                                                <  2:146586/149‑1 (MQ=255)
                                         tgtCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCa                                                                                                         <  2:42090/149‑1 (MQ=255)
                                         tgtCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCa                                                                                                         <  1:58862/149‑1 (MQ=255)
                                              aaGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACATGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCg                                                                                                    >  1:220813/1‑149 (MQ=255)
                                                     tGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGcac                                                                                             <  3:68530/149‑1 (MQ=255)
                                                               atCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGa                                                                                     >  3:270518/1‑147 (MQ=255)
                                                                  gAAGATGGCGCTGATAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTTTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAgg                                                                                 <  1:93318/148‑1 (MQ=255)
                                                                  gAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGGCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAggg                                                                                <  1:124894/149‑1 (MQ=255)
                                                                    aGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACATGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAggggg                                                                              <  2:220813/149‑1 (MQ=255)
                                                                          cgcTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTGCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCt                                                                         >  1:304244/1‑149 (MQ=255)
                                                                            cTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAg                                                                      >  6:142953/1‑149 (MQ=255)
                                                                                          aCCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAgg                                                                                 <  8:1009/124‑1 (MQ=255)
                                                                                          aCCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAgg                                                                                 >  7:1009/1‑124 (MQ=255)
                                                                                          aCCGTGAAACAAACGCCTTGGCTGTCGCCCCTTGG‑CGGGGCGGCAGCCCGCTCGCCTGCAACAGGCGAGAACGGTGTGGGAGCTGCAGCCGGGGCAGTCCGGGCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCagag                                                         <  4:270518/148‑1 (MQ=255)
                                                                                                  agaaaAGCCTTGGCTGTGGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGGGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAggcgg                                                <  2:304244/149‑1 (MQ=255)
                                                                                                           ttggaggTTGCCCCTTGT‑CGTTGCGTCAGGCCTCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGt                                                       <  4:278725/127‑1 (MQ=255)
                                                                                                           ttGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGt                                                       >  3:278725/1‑133 (MQ=255)
                                                                                                              gCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCgggg                                    >  2:114180/1‑149 (MQ=255)
                                                                                                                       ccTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCagag                           >  1:222443/1‑149 (MQ=255)
                                                                                                                        cTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCagag                           >  1:19768/1‑148 (MQ=255)
                                                                                                                           gT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGc                                   >  7:171903/1‑137 (MQ=255)
                                                                                                                                     tCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAAcc              >  8:166468/1‑149 (MQ=255)
                                                                                                                                       aGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGt            >  4:326091/1‑149 (MQ=255)
                                                                                                                                         gCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGAGGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTg          >  1:332718/1‑149 (MQ=255)
                                                                                                                                              tcGCCTGTTGCAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTgcggc      >  3:372736/1‑148 (MQ=255)
                                                                                                                                                 ccTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAgg  >  6:363953/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CAGAGAAGCTTGCTTGAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAGG  >  NC_002947/2336349‑2336641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑