| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,336,498 | A→T | 13.5% | intergenic (‑42/+38) | PP_2052 ← / ← PP_5500 | bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,336,498 | 0 | A | T | 13.5% | 108.7 / 6.8 | 44 | intergenic (‑42/+38) | PP_2052/PP_5500 | bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (17/21); new base T (3/3); total (20/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
CTTGCTTGAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAGG > NC_002947/2336357‑2336641 | cttgcttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCg < 3:283168/149‑1 (MQ=255) cttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgaga < 5:232862/148‑1 (MQ=255) gcctgtCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACgg < 5:167362/146‑1 (MQ=255) gAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACg < 1:21160/148‑1 (MQ=255) aGTGTCCTGAAGCGCAGGCGCTCGGTGTCGACGAGGGTGCCATCCTAATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgt < 4:294103/149‑1 (MQ=255) tgtCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgt < 5:133792/149‑1 (MQ=255) tCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCTTCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgtgt > 2:93318/1‑149 (MQ=255) tCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgtgt < 4:362559/149‑1 (MQ=255) tCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgtgt < 3:315084/149‑1 (MQ=255) cTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgtgt > 1:146586/1‑147 (MQ=255) tGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgagat > 2:61271/1‑137 (MQ=255) tGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgagat > 2:67139/1‑137 (MQ=255) tGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgagat < 1:61271/138‑2 (MQ=255) tGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgagat < 1:67139/138‑2 (MQ=255) aaGCGCTGGCGATCGGTGTCGACGTGGGTGCCAACCATATCGAAGATGGCGCTGAAAATCTGTTTACGGTGAAAGAAAATCTTTTGGTTTTGCCCGTTTT‑CGTTGCTTATGGCATCTGGCGTTCAACTTGGGTTAACGGTGTGTGAg < 4:67901/147‑1 (MQ=255) aGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTg > 2:58862/1‑149 (MQ=255) ggCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTgg < 5:247836/149‑1 (MQ=255) cgcTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTgggg < 5:313372/149‑1 (MQ=255) cgcTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTgggg < 2:146586/149‑1 (MQ=255) tgtCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCa < 2:42090/149‑1 (MQ=255) tgtCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCa < 1:58862/149‑1 (MQ=255) aaGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACATGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCg > 1:220813/1‑149 (MQ=255) tGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGcac < 3:68530/149‑1 (MQ=255) atCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGa > 3:270518/1‑147 (MQ=255) gAAGATGGCGCTGATAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTTTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAgg < 1:93318/148‑1 (MQ=255) gAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGGCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAggg < 1:124894/149‑1 (MQ=255) aGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACATGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAggggg < 2:220813/149‑1 (MQ=255) cgcTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTGCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCt > 1:304244/1‑149 (MQ=255) cTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAg > 6:142953/1‑149 (MQ=255) aCCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAgg > 7:1009/1‑124 (MQ=255) aCCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAgg < 8:1009/124‑1 (MQ=255) aCCGTGAAACAAACGCCTTGGCTGTCGCCCCTTGG‑CGGGGCGGCAGCCCGCTCGCCTGCAACAGGCGAGAACGGTGTGGGAGCTGCAGCCGGGGCAGTCCGGGCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCagag < 4:270518/148‑1 (MQ=255) agaaaAGCCTTGGCTGTGGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGGGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAggcgg < 2:304244/149‑1 (MQ=255) ttggaggTTGCCCCTTGT‑CGTTGCGTCAGGCCTCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGt < 4:278725/127‑1 (MQ=255) ttGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGt > 3:278725/1‑133 (MQ=255) gCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCgggg > 2:114180/1‑149 (MQ=255) ccTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCagag > 1:222443/1‑149 (MQ=255) cTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCagag > 1:19768/1‑148 (MQ=255) gT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGc > 7:171903/1‑137 (MQ=255) tCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAAcc > 8:166468/1‑149 (MQ=255) aGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGt > 4:326091/1‑149 (MQ=255) gCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGAGGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTg > 1:332718/1‑149 (MQ=255) tcGCCTGTTGCAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTgcggc > 3:372736/1‑148 (MQ=255) ccTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAgg > 6:363953/1‑149 (MQ=255) | CTTGCTTGAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGT‑CGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAGG > NC_002947/2336357‑2336641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |