Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,395,035 G→A 7.2% L120L (CTG→TTG)  dacB ← D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,395,0350GA 7.2% 158.4 / 2.2 55L120L (CTG→TTG) dacBD‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base G (23/28);  new base A (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

GGTTGACCAGCAGCGAGTCGGGCTTGACCAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGCACGCCGTTGCTCAGGGTGCCGTCGGTGTAGAACTCGGTTTTCCACTGGAAGGTCGGGC  >  NC_002947/2394888‑2395172
                                                                                                                                                   |                                                                                                                                         
ggTTGACCAGCAGCGAGTCGGGCTTGACCAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAg                                                                                                                                           <  4:22389/148‑1 (MQ=255)
ggTTGACCAGCAGCGAGTCGGGCTTGACCAGGAACGGCTTGTTTTCAACACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAgg                                                                                                                                          >  4:6422/1‑149 (MQ=255)
    gACCAGCAGCGAGTCGGGCTTGACCAGGAACGCCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCAc                                                                                                                                      >  1:72959/1‑149 (MQ=255)
     aCCAGCAGCGAGTCGGGCTTGACCAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACg                                                                                                                                     >  3:307950/1‑149 (MQ=255)
      ccAGCAGCGAGTCGGGCTTGACCAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGc                                                                                                                                    <  1:376279/149‑1 (MQ=255)
         gcagcGAGTCGGGCTTGACCAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCAt                                                                                                                                  >  1:400810/1‑148 (MQ=255)
         gcagcGAGTCGGGCTTGACCAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATc                                                                                                                                 <  2:34598/149‑1 (MQ=255)
              gAGTCGGGCTTGACCAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATcagcag                                                                                                                            <  4:126594/149‑1 (MQ=255)
              gAGTCGGGCTTGACCAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATcagcag                                                                                                                            <  4:307950/149‑1 (MQ=255)
               aGTCGGGCTTGACCAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCagcagc                                                                                                                           <  2:12220/149‑1 (MQ=255)
                gTCGGGCTTGACTAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACAAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCagcagc                                                                                                                           >  1:331439/1‑148 (MQ=255)
                  cGGGCTTGACCAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCcac                                                                                                                        <  6:285425/149‑1 (MQ=255)
                          aCCAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCtttt                                                                                                                 <  7:221597/148‑1 (MQ=255)
                          aCCAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCtttt                                                                                                                 <  2:400810/148‑1 (MQ=255)
                           ccAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTcc                                                                                                               <  8:5801/149‑1 (MQ=255)
                           ccAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAAGTCACGCATCAGCAGCCACAGCTTTTc                                                                                                                >  5:29540/1‑148 (MQ=255)
                           ccAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAAGTCACGCATCAGCAGCCACAGCTTTTc                                                                                                                >  5:23675/1‑148 (MQ=255)
                                aaCGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAAGTCACGCATCAGCAGCCACAGCTTTTCCATGtt                                                                                                          <  6:23675/149‑1 (MQ=255)
                                aaCGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAAGTCACGCATCAGCAGCCACAGCTTTTCCATGtt                                                                                                          <  6:29540/149‑1 (MQ=255)
                                         ttttCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTggg                                                                                                 <  2:72959/149‑1 (MQ=255)
                                         ttttCAACACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTggg                                                                                                 <  3:6422/149‑1 (MQ=255)
                                            tcatcaCCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGc                                                                                                                     <  3:355573/126‑1 (MQ=255)
                                            tcatcaCCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGc                                                                                                                     >  4:355573/1‑126 (MQ=255)
                                             catcaCCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGATTTTCCATGTTCAGTTTGGGGTCa                                                                                             >  2:142777/1‑149 (MQ=255)
                                                 aCCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGc                                                                                         >  1:338686/1‑149 (MQ=255)
                                                        tcgtcgtTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCAc                                                                                            <  5:389485/139‑1 (MQ=255)
                                                        tcgtcgtTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCAc                                                                                            >  6:389485/1‑139 (MQ=255)
                                                        tcgtcgtTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCtt                                                                                   >  2:191359/1‑148 (MQ=255)
                                                          gtcgtTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTaa                                                                                <  1:191359/149‑1 (MQ=255)
                                                             gtTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAgg                                                                              >  7:228704/1‑148 (MQ=255)
                                                                gAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTg                                                                                                   >  8:6201/1‑124 (MQ=255)
                                                                gAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTg                                                                                                   <  7:6201/124‑1 (MQ=255)
                                                                 aaCTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTgg                                                                                                  >  8:198850/1‑124 (MQ=255)
                                                                 aaCTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTgg                                                                                                  <  7:198850/124‑1 (MQ=255)
                                                                  aCTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAgg                                                                        >  6:67229/1‑149 (MQ=255)
                                                                      cGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTAc                                                                    >  4:88671/1‑149 (MQ=255)
                                                                                     cACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGCACGCCGt                                                     <  2:338686/149‑1 (MQ=255)
                                                                                      ccGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCAt                                                                                                             <  5:237659/91‑1 (MQ=255)
                                                                                      ccGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCAt                                                                                                             >  6:237659/2‑92 (MQ=255)
                                                                                              gCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGCACGCCGTTGCTCAggg                                            >  2:339613/1‑149 (MQ=255)
                                                                                              gCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGCACGCCGTTGCTCAgg                                             <  3:88671/148‑1 (MQ=255)
                                                                                              gCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGCACGCCGTTGCTCAgg                                             <  3:68223/148‑1 (MQ=255)
                                                                                                 gCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCGTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGCGGTTACCGTTAAGCACGCCGTTCCTCAGGGTGc                                         >  7:190225/1‑149 (MQ=255)
                                                                                                      ccAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGCACGCCGTTGCTCAGGGTGCCGTCg                                    <  8:228704/149‑1 (MQ=255)
                                                                                                       cAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGCACGCCGTTGCTCAGGGTGCCGTCg                                    <  2:331439/148‑1 (MQ=255)
                                                                                                          cACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTAcc                                                                   >  3:123073/1‑114 (MQ=255)
                                                                                                          cACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTAcc                                                                   <  4:123073/114‑1 (MQ=255)
                                                                                                            ccAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGCACGCCGTTGCTCAGGGTGCCGTCGGTGTAg                              <  1:142777/149‑1 (MQ=255)
                                                                                                                   aCCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGCACGCCGTTGCTCAGGGTGCCGTCGGTGTAGAACTCgg                       >  1:380483/1‑149 (MQ=255)
                                                                                                                   aCCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGC‑TTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGCACGCCGTTGCTCAGGGTGCCGTCGGTGTAGAACTCGGt                      >  5:373068/1‑149 (MQ=255)
                                                                                                                     cGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGc                                                            >  7:153868/1‑110 (MQ=255)
                                                                                                                     cGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGc                                                            <  8:153868/110‑1 (MQ=255)
                                                                                                                      ggTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGCACGCCGTTGCTCAGGGTGCCGTCGGTGTAGAACTCGGttt                    <  5:67229/149‑1 (MQ=255)
                                                                                                                          aCGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGC‑TTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGCACGCCGTTGCTCAGGGTGCCGTCGGTGTAGAACTCGGTTTTCCa                <  6:373068/148‑1 (MQ=255)
                                                                                                                                   aCGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTATAACCAACAGGTGCTCGTTAAGGACGCCGTTGCTCACGGTGCCGTCGGTGTAGAACTCGGTTTTCCACTGGAAGGt       >  1:335641/1‑149 (MQ=255)
                                                                                                                                       cATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATCTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTAAAGCACGCCGTTGCTCAGGGTGCCGTCGGTGTAGAACTCGGTTTTCCACt              >  3:291320/1‑138 (MQ=255)
                                                                                                                                        aTTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGCACGCCGTTGCTCAGGGTGCCGTCGGTGTAGAACTCGGTTTTCCACTGGAAGGTCGGGc  <  2:380483/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
GGTTGACCAGCAGCGAGTCGGGCTTGACCAGGAACGGCTTGTTTTCATCACCGCCGTCGTCGTTGAACTGCGGCAGGTTGGGTTGCACGAAGTGGCTGCGGTCCAGCACCAGGTCACCGGTGACGGTGCGCACGCCATTGGCGCGCAGGTCACGCATCAGCAGCCACAGCTTTTCCATGTTCAGTTTGGGGTCACCGCCACCTTTAAGGTAGAGGTTACCGTTAAGCACGCCGTTGCTCAGGGTGCCGTCGGTGTAGAACTCGGTTTTCCACTGGAAGGTCGGGC  >  NC_002947/2394888‑2395172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑