Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,445,045 T→A 7.3% E156V (GAA→GTA) ‡ lexA‑I ← transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,445,0450TA 7.3% 244.2 / 7.7 84E156V (GAA→GTA) ‡lexA‑Itranscriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (39/38);  new base A (3/3);  total (42/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

CTGGATCAGCGGCGAATGACGCCGACGCTCAAGCCCTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTCGGCTTGGGGGTGGAAGAA  >  NC_002947/2444898‑2445190
                                                                                                                                                   |                                                                                                                                                 
ctGGATCAGCGGCGAATGACGCCGACGCTCAAGCCCTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTc                                                                                                                                                  >  8:338170/1‑149 (MQ=255)
           gcgAATGACGCCGACGCTCAAGCCCTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACGCAGTCACCgtcaa                                                                                                                                       <  5:148476/149‑5 (MQ=255)
           gcgAATGACGCCGACGCTCAAGCCCTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACGAAGTCACCgtcaa                                                                                                                                       <  4:10325/149‑5 (MQ=255)
           gcgAATGACGCCGACGCTCAAGCCCTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGGGACGAAGTCACCgtcaa                                                                                                                                       <  3:75417/149‑5 (MQ=255)
                    gCCGACGCTCAAGCCCTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCACGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGGCTTCGTCACCGATGCGCGCCACaa                                                                                                                              >  1:294601/1‑149 (MQ=255)
                    gCCGACGCTCAAGCCCTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCACGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACaa                                                                                                                              >  2:382343/1‑149 (MQ=255)
                      cGACGCTCAAGCCCTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCca                                                                                                                                 <  4:160335/144‑1 (MQ=255)
                      cGACGCTCAAGCCCTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCca                                                                                                                                 >  3:160335/1‑144 (MQ=255)
                            tCAAGCCCTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTgg                                                                                                                      >  8:71391/1‑149 (MQ=255)
                               aGCCCTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCg                                                                                                                   >  7:298451/1‑149 (MQ=255)
                                 cccTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGtt                                                                                                                 <  7:338170/149‑1 (MQ=255)
                                  ccTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAATGATCTGGCCGTTg                                                                                                                >  4:41141/1‑149 (MQ=255)
                                  ccTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGATGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTg                                                                                                                >  8:299690/1‑149 (MQ=255)
                                        tCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCt                                                                                                          >  4:17715/1‑149 (MQ=255)
                                          aCCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCtt                                                                                                         <  7:41317/148‑1 (MQ=255)
                                           ccAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGTCCGTTGCGGGCTTCa                                                                                                       >  6:280366/1‑149 (MQ=255)
                                           ccAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCa                                                                                                       >  2:250880/1‑149 (MQ=255)
                                           ccAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCa                                                                                                       <  7:71391/149‑1 (MQ=255)
                                           ccAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCa                                                                                                       <  3:187818/149‑1 (MQ=255)
                                            cAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCAc                                                                                                      <  7:237651/149‑1 (MQ=255)
                                                tCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACgg                                                                                                    >  7:2304/1‑147 (MQ=255)
                                                  cTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAgg                                                                                                >  2:26694/1‑149 (MQ=255)
                                                  cTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAg                                                                                                 <  7:299690/148‑1 (MQ=255)
                                                   tGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGgt                                                                                               >  3:322921/1‑149 (MQ=255)
                                                     ttCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGgtgt                                                                                             <  1:250880/149‑1 (MQ=255)
                                                       cTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGtgtg                                                                                            >  6:131033/1‑148 (MQ=255)
                                                          tCATGTCGACTTCGATGGGGGCGAATTCGGTGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCAc                                                                                         <  2:296154/148‑1 (MQ=255)
                                                          tCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGc                                                                                           <  3:374701/146‑1 (MQ=255)
                                                          tCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCAc                                                                                         <  1:306920/148‑1 (MQ=255)
                                                          tCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCACGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCAc                                                                                         <  1:382343/148‑1 (MQ=255)
                                                              gTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACTGCAGGTTTGCACAGCca                                                                                    >  6:337399/1‑149 (MQ=255)
                                                              gTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGc                                                                                      >  6:252936/1‑147 (MQ=255)
                                                               tCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCcag                                                                                   <  4:144682/149‑1 (MQ=255)
                                                                 gACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAATGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCcagc                                                                                  <  3:41141/148‑1 (MQ=255)
                                                                 gACTGCGATGGGGGAGAATTCGGGGTTGTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGACGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCcagc                                                                                  <  6:321050/148‑1 (MQ=255)
                                                                  aCTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCcagcag                                                                                <  5:131033/149‑1 (MQ=255)
                                                                   cTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAgg                                                                               >  2:64660/1‑149 (MQ=255)
                                                                       gATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGc                                                                           >  6:63302/1‑149 (MQ=255)
                                                                             ggCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGa                                                                     <  1:26694/149‑1 (MQ=255)
                                                                              gCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGaa                                                                    <  5:146304/149‑1 (MQ=255)
                                                                                gAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCATTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCg                                                                      >  6:346661/1‑145 (MQ=255)
                                                                                  aTTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATg                                                                <  6:213558/149‑1 (MQ=255)
                                                                                  aTTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATg                                                                <  1:64660/149‑1 (MQ=255)
                                                                                  aTTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATg                                                                <  5:280366/149‑1 (MQ=255)
                                                                                   ttCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGc                                                               >  5:311915/1‑149 (MQ=255)
                                                                                     cGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGcc                                                              <  3:392081/148‑1 (MQ=255)
                                                                                          ttttCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGt                                                         >  7:153305/1‑148 (MQ=255)
                                                                                           tttCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTcc                                                       >  8:271333/1‑149 (MQ=255)
                                                                                              cGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCtt                                                     >  6:67989/1‑148 (MQ=255)
                                                                                               ggCAAGCTGCTAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGTGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTtca                                                   <  5:337399/149‑1 (MQ=255)
                                                                                                 cAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTtcatg                                                 <  6:311915/149‑1 (MQ=255)
                                                                                                 cAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTtcatg                                                 <  8:298451/149‑1 (MQ=255)
                                                                                                 cAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTtcatg                                                 >  7:241940/1‑149 (MQ=255)
                                                                                                      agcCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTtcatgctcat                                            >  5:180613/1‑149 (MQ=255)
                                                                                                           gACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCgt                                       >  3:18428/1‑149 (MQ=255)
                                                                                                                   tgcCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTAcgcg                                >  2:151756/1‑148 (MQ=255)
                                                                                                                   tgcCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTAcgcg                                >  3:338754/1‑148 (MQ=255)
                                                                                                                    gcCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTAcgcg                                >  2:169028/1‑147 (MQ=255)
                                                                                                                     ccTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACgcgc                               <  8:241940/147‑1 (MQ=255)
                                                                                                                      cTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCgcagc                            <  5:252936/149‑1 (MQ=255)
                                                                                                                       ttCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCgcagc                            >  6:69527/1‑148 (MQ=255)
                                                                                                                         cgcgCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCaa                          <  1:190003/148‑1 (MQ=255)
                                                                                                                            gcTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAg                      >  4:327957/1‑149 (MQ=255)
                                                                                                                            gcTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAg                      <  3:327957/149‑1 (MQ=255)
                                                                                                                            gcTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAg                      >  1:152105/1‑149 (MQ=255)
                                                                                                                             cTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGt                     >  8:31976/1‑149 (MQ=255)
                                                                                                                              ttGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTc                    >  6:352394/1‑149 (MQ=255)
                                                                                                                                gAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTCgg                  >  8:134406/1‑149 (MQ=255)
                                                                                                                                gAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCATTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTCg                   <  5:346661/148‑1 (MQ=255)
                                                                                                                                 aaGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTCgg                  <  7:271333/148‑1 (MQ=255)
                                                                                                                                     gcTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTCGGCTTgg             <  4:322921/149‑1 (MQ=255)
                                                                                                                                       gcatcGGTGACGAAGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTCGGCTTgggg           >  8:88442/5‑149 (MQ=255)
                                                                                                                                         atcGGTGACGAAGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGgt                                                                                               >  2:81299/3‑63 (MQ=38)
                                                                                                                                         atcGGTGACGAAGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGgt                                                                                               <  1:81299/61‑1 (MQ=38)
                                                                                                                                          tcGGTGACGAAGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTCGGCTTGGGGGTg        >  3:44727/2‑149 (MQ=255)
                                                                                                                                          aCGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTCGGCTTGGGGGt         <  5:67989/148‑1 (MQ=255)
                                                                                                                                          aCGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTCGGCTTGGGGGTg        >  4:85794/1‑149 (MQ=255)
                                                                                                                                          aCGGTGACTTCGACACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCaaa                         <  4:180204/132‑1 (MQ=255)
                                                                                                                                          aCGGTGACTTCGACACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCaaa                         >  3:180204/1‑132 (MQ=255)
                                                                                                                                              tGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTCGGCTTGGGGGTGgaag    <  2:152105/149‑1 (MQ=255)
                                                                                                                                               gACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTc                    >  4:393829/1‑132 (MQ=255)
                                                                                                                                               gACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTc                    <  3:393829/132‑1 (MQ=255)
                                                                                                                                                aCTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTCGGCTTGGGGGTGgaagaa  <  8:2304/149‑1 (MQ=255)
                                                                                                                                                aCTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTCGGCTTGGGGGTGgaagaa  <  1:162520/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CTGGATCAGCGGCGAATGACGCCGACGCTCAAGCCCTCGATCACCAGCTCCTGTTCTTTCAGGTCGACTTCGATGGGGGCGAATTCGGGGTTTTCGGCAAGCAGCCAGACTTTGCTGCCTTCGCGCTTGAAGCGCTTGACGGTGACTTCGTCACCGATGCGCGCCACAACGATCTGGCCGTTGCGGGCTTCACGGCAGGTGTGCACCGCCAGCAGGTCGCCGTCGAAGATGCCGACGTCCTTCATGCTCATGCCGTGTACGCGCAGCAAATAGTCGGCTTGGGGGTGGAAGAA  >  NC_002947/2444898‑2445190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑