Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,453,608 T→C 5.3% D504G (GAC→GGC)  mfd ← transcription‑repair coupling factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,453,6080TC 5.3% 223.8 / 2.1 75D504G (GAC→GGC) mfdtranscription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base T (32/39);  new base C (2/2);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

CTGCCAGGCCTCCGAACCCAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTCGCCGCGCTTGTCACGGCGCCGGCGCTGCATCAC  >  NC_002947/2453460‑2453756
                                                                                                                                                     |                                                                                                                                                    
cTGCCAGGCCTCCGAACCCAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGt                                                                                                                                                      >  5:90018/1‑149 (MQ=255)
  gCCAGGCCTCCGAACCCAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTc                                                                                                                                                     >  4:191305/1‑148 (MQ=255)
   ccAGGCCTCCGAACCCAGCCGGTGCAGCGGTGCCAGGTCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGt                                                                                                                                                      <  1:357661/146‑1 (MQ=255)
   ccAGGCCTCCGAACCCAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGa                                                                                                                                                   >  7:287170/1‑149 (MQ=255)
   ccAGGCCTCCGAACCCAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGa                                                                                                                                                   >  7:124970/1‑149 (MQ=255)
    cAGGCCTCCGAACCCAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGAt                                                                                                                                                  <  8:30112/149‑1 (MQ=255)
     aGGCCTCCGAACCCAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGACGGCCAGGCCGCCGaat                                                                                                                                                 <  4:199882/149‑3 (MQ=255)
         cTCCGAACCCAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTc                                                                                                                                               >  4:145575/1‑147 (MQ=255)
         cTCCGAACCCAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCa                                                                                                                                             <  7:140113/149‑1 (MQ=255)
            cGAACCCAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCg                                                                                                                                          >  2:209967/1‑149 (MQ=255)
            cGAACCCAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCg                                                                                                                                          >  2:216700/1‑149 (MQ=255)
                cccAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGGTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGc                                                                                                                                      <  7:47542/149‑1 (MQ=255)
                 ccAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGcc                                                                                                                                     <  2:395970/149‑1 (MQ=255)
                  cAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGc                                                                                                                                      >  5:353254/1‑147 (MQ=255)
                   aGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAg                                                                                                                                   <  1:216700/149‑1 (MQ=255)
                   aGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAg                                                                                                                                   <  1:209967/149‑1 (MQ=255)
                      cGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGcc                                                                                                                                >  2:25655/1‑149 (MQ=255)
                       ggTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGccc                                                                                                                               <  3:55440/149‑1 (MQ=255)
                        gTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCa                                                                                                                              <  4:374732/149‑1 (MQ=255)
                           cAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAgg                                                                                                                            <  5:33371/148‑1 (MQ=255)
                              cGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGc                                                                                                                                           >  7:300118/1‑130 (MQ=255)
                              cGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGAACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGc                                                                                                                                           <  8:300118/130‑1 (MQ=255)
                                gTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CCGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCgg                                                                                                                      <  1:287405/149‑1 (MQ=255)
                                         cgtcgtCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCa                                                                                                             <  8:124970/149‑1 (MQ=255)
                                          gtcgtcGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCAt                                                                                                            >  5:44146/1‑149 (MQ=255)
                                              tcgCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTc                                                                                                        <  3:191305/149‑1 (MQ=255)
                                                gCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCg                                                                                                       <  6:90018/148‑1 (MQ=255)
                                                 cTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGAt                                                                                                     <  4:61679/149‑1 (MQ=255)
                                                 cTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGAt                                                                                                     <  3:194772/149‑1 (MQ=255)
                                                     cGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGAtgtg                                                                                                  >  7:68551/1‑148 (MQ=255)
                                                       gtgtAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGcac                                                                                               <  8:287170/149‑1 (MQ=255)
                                                            gCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCg                                                                                          <  3:145575/149‑1 (MQ=255)
                                                             cGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGcactagta                                                                                          >  8:221339/1‑143 (MQ=255)
                                                                 cGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCa                                                                                     <  6:353254/149‑1 (MQ=255)
                                                                  gATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTAGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGc                                                                                                                                      >  1:377170/1‑99 (MQ=255)
                                                                  gATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTAGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGc                                                                                                                                      <  2:377170/99‑1 (MQ=255)
                                                                    tCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGCTCGATGTGCACCACCGGCGCACCt                                                                                  >  4:307704/1‑149 (MQ=255)
                                                                     cAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCg                                                                                                                   <  3:58682/115‑1 (MQ=255)
                                                                     cAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCg                                                                                                                   >  4:58682/1‑115 (MQ=255)
                                                                       ggTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTcg                                                                               <  1:25655/149‑1 (MQ=255)
                                                                         tGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGTGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTcgcg                                                                             <  5:299645/149‑1 (MQ=255)
                                                                         tGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTcgcg                                                                             >  8:239288/1‑149 (MQ=255)
                                                                          gCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTcgcg                                                                             >  6:24452/1‑148 (MQ=255)
                                                                                 ggCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCg                                                                      <  5:47267/148‑1 (MQ=255)
                                                                                   ccACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGt                                                                    >  2:221285/1‑148 (MQ=255)
                                                                                      ccGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAgg                                                                <  4:57618/149‑1 (MQ=255)
                                                                                         gCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTc                                                             <  4:90428/149‑1 (MQ=255)
                                                                                         gCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTc                                                             >  3:338152/1‑149 (MQ=255)
                                                                                         gCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTc                                                             >  3:90428/1‑149 (MQ=255)
                                                                                          cacaTACAGCTTGGCGTTCTCGGCGTATTCAAGCGTCAGAAATT‑CGGCGGCCTGTCCGTCGAATTCCAGAGTTGCCAGTCCCACGTAGCGGCCGACGCCATGGTCGATCTGAACCACAGGCGCACCTTCGCGCTGCTCGGTGACGTTCt                                                            >  7:325821/1‑149 (MQ=255)
                                                                                             aTACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTtga                                                         <  3:307704/149‑1 (MQ=255)
                                                                                               aCAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATTACGCCGGCATGGCCGTCGATTTCCAGCGTGGCCAGGCCAAGGTACCGGCCGACGCCATGGACGATGTGCACCACCGGCGCACCTACGCGCAGCTCGGTGAGGTTCTtgat                                                        >  1:277381/1‑149 (MQ=255)
                                                                                                 aGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGAc                                                     <  6:44146/149‑1 (MQ=255)
                                                                                                       gCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGcaccac                                                                                            >  8:14051/1‑104 (MQ=255)
                                                                                                       gCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGcaccac                                                                                            <  7:14051/104‑1 (MQ=255)
                                                                                                               ggCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTtga                                                         <  1:351828/131‑1 (MQ=255)
                                                                                                               ggCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTtga                                                         >  2:351828/1‑131 (MQ=255)
                                                                                                                gCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCAGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGt                                      <  1:203876/149‑1 (MQ=255)
                                                                                                                    aTTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAgg                                                                >  1:112451/1‑119 (MQ=255)
                                                                                                                    aTTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAgg                                                                <  2:112451/119‑1 (MQ=255)
                                                                                                                        cAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGcgtc                                               >  1:282642/1‑132 (MQ=255)
                                                                                                                        cAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGcgtc                                               <  2:282642/132‑1 (MQ=255)
                                                                                                                        cAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTcg                                  >  4:94465/1‑145 (MQ=255)
                                                                                                                        cAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTcg                                  <  3:94465/145‑1 (MQ=255)
                                                                                                                            gTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTCGCCGCGCtt                          <  7:331412/149‑1 (MQ=255)
                                                                                                                            gTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTCGCCGCGCtt                          <  2:277381/149‑1 (MQ=255)
                                                                                                                              ctggAAAT‑CGACGGCCAGGCCGCCGAATTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTCGCCGCGCTTGt                        >  6:28702/3‑149 (MQ=255)
                                                                                                                                ggAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTCGCCGCGCTTGTCa                      >  6:183357/1‑149 (MQ=255)
                                                                                                                                 gAAAT‑CGACGGCCAGGCCGCCGAATTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAg                                                                 >  4:54396/1‑105 (MQ=38)
                                                                                                                                 gAAAT‑CGACGGCCAGGCCGCCGAATTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAg                                                                 <  3:54396/105‑1 (MQ=38)
                                                                                                                                  aaTT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTCGCCGCGCTTGTCAcg                    <  7:239288/149‑1 (MQ=255)
                                                                                                                                    tt‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTCGCCGCGCTTGTCAcggc                  <  4:338152/149‑1 (MQ=255)
                                                                                                                                         gcggcCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTCGCCgcgc                            <  8:273976/135‑1 (MQ=255)
                                                                                                                                         gcggcCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTCGCCgcgc                            >  7:273976/1‑135 (MQ=255)
                                                                                                                                               tGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTCGCCGCGCTTGTCACGGCGCCGGCGCTg        >  7:84493/1‑149 (MQ=255)
                                                                                                                                                 gCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTCGCCGCGCTTGTCACGGCGCCGGCGCTGCa      >  7:260609/1‑149 (MQ=255)
                                                                                                                                                   cGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTCGCCGCGCTTGTCACGGCGCCGGCGCTGCAt     >  3:348417/1‑148 (MQ=255)
                                                                                                                                                     tCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTCGCCGCGCTTGTCACGGCGCCGGCGCTGCATCAc  >  7:70000/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTGCCAGGCCTCCGAACCCAGCCGGTGCAGCGGTGCCAGGGCGTCGTCGCTGCCGGTGTAGCGGGCGATCAGGTGCAGGTTGGCCACCGGCACATACAGCTTGGCGTTCTCGGCGTATTCCAGCGTCAGGAATT‑CGGCGGCCTGGCCGTCGATTTCCAGCGTGGCCAGGCCCAGGTAGCGGCCGACGCCATGGTCGATGTGCACCACCGGCGCACCTTCGCGCAGCTCGGTGAGGTTCTTGATGACCGCGTCGTTGGCGGTTTCGCCGCGCTTGTCACGGCGCCGGCGCTGCATCAC  >  NC_002947/2453460‑2453756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑