Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,460,731 G→C 6.7% L25V (CTG→GTG)  PP_2153 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,460,7310GC 6.7% 188.2 / 3.2 60L25V (CTG→GTG) PP_2153hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/29);  new base C (2/2);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01

CCGCCTTCGGAAATGACCACCGGCAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGAAGTGCGATCCGATCTTCGATGCGGTAGT  >  NC_002947/2460583‑2460874
                                                                                                                                                    |                                                                                                                                               
ccgccTTCGGAAATGACCACCGGCAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAg                                                                                                                                                 <  1:258657/149‑1 (MQ=255)
 cgccTTCGGAAATGACCACCGGCAGCGGCTCGCCGCCTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAgg                                                                                                                                                >  4:172705/1‑149 (MQ=255)
  gccTTCGGAAATGACCACCGGCAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCACTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGACCTTGTCGCTTAACTGGCGCAACAGgt                                                                                                                                               >  3:274891/1‑149 (MQ=255)
     ttCGGAAATGACCACCGGCAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGtt                                                                                                                                            >  5:182629/1‑149 (MQ=255)
        ggAAATGACCACCGGCAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGc                                                                                                                                          >  6:210299/1‑148 (MQ=255)
        ggAAATGACCACCGGCAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGc                                                                                                                                          <  4:1682/148‑1 (MQ=255)
        ggAAATGACCACCGGCAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGc                                                                                                                                          >  3:281975/1‑148 (MQ=255)
           aaTGACCACCGGCAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACt                                                                                                                                      <  3:378490/149‑1 (MQ=255)
           aaTGACCACCGGCAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACACCTGTTGCgcca                                                                                                                                      <  5:242134/149‑4 (MQ=255)
            aTGACCACCGGCAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGTCGCAACACCTGTTGCgccag                                                                                                                                     <  6:45891/149‑5 (MQ=255)
                  accGGCAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTc                                                                                                                               <  5:326760/149‑1 (MQ=255)
                   ccGGCAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCg                                                                                                                              <  7:323457/149‑1 (MQ=255)
                      gCAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGc                                                                                                                            >  4:111497/1‑148 (MQ=255)
                       cAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGc                                                                                                                                          <  8:129179/133‑1 (MQ=255)
                       cAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGc                                                                                                                                          >  7:129179/1‑133 (MQ=255)
                             cTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTg                                                                                                                      >  4:92972/1‑147 (MQ=255)
                                gCCGACTTCGCCATGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGGCAGGTGGAGc                                                                                                                  <  8:50753/148‑1 (MQ=255)
                                    aCTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCt                                                                                                             <  6:244308/149‑1 (MQ=255)
                                    aCTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCt                                                                                                             <  3:131982/149‑1 (MQ=255)
                                      ttCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCt                                                                                                             >  7:161532/1‑147 (MQ=255)
                                      ttCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCt                                                                                                             >  7:258643/1‑147 (MQ=255)
                                        cGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGa                                                                                                           <  7:35128/147‑1 (MQ=255)
                                          ccAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAg                                                                                                       >  4:359614/1‑149 (MQ=255)
                                            aaGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGt                                                                                                     >  7:237259/1‑149 (MQ=255)
                                                       gCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAgcggc                                                                                          >  1:87087/1‑149 (MQ=255)
                                                        cccAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTGGCTTACCTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGAAGCTCGCTGAGCAGGTTGAACAgcggc                                                                                          >  4:242913/1‑148 (MQ=255)
                                                         ccAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAgcggcg                                                                                         >  8:52604/1‑148 (MQ=255)
                                                         ccAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGa                                                                                        <  3:111497/149‑1 (MQ=255)
                                                                 cgcgCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCtt                                                                                >  7:303833/1‑149 (MQ=255)
                                                                              tCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGACCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTgg                                                                   <  4:274891/149‑1 (MQ=255)
                                                                                    cgcTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAg                                                             <  3:172705/149‑1 (MQ=255)
                                                                                       ttGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGc                                                          <  8:303833/149‑1 (MQ=255)
                                                                                         gTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCt                                                                     >  6:45863/1‑136 (MQ=255)
                                                                                         gTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCt                                                                     <  5:45863/136‑1 (MQ=255)
                                                                                          ttCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGAGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTc                                                       <  5:391662/149‑1 (MQ=255)
                                                                                            cTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCg                                                      <  8:237259/148‑1 (MQ=255)
                                                                                               tgCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCcg                                                  <  2:87087/149‑1 (MQ=255)
                                                                                               tgCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCACTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTAGCAACACATCTGGTTCAAGGGCTTCGGCcg                                                  >  1:332707/1‑149 (MQ=255)
                                                                                                gCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCcgc                                                 >  8:236975/1‑149 (MQ=255)
                                                                                                   cGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAAAACATCAGGTTAAAGGGCTTCCGCCGCGCt                                              >  8:168208/1‑149 (MQ=255)
                                                                                                    gCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCt                                              >  1:33645/1‑148 (MQ=255)
                                                                                                    gCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCt                                              >  6:278693/1‑148 (MQ=255)
                                                                                                         aaGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAgggg                                        >  7:90993/1‑149 (MQ=255)
                                                                                                              ggcgAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTa                                   >  4:130862/1‑149 (MQ=255)
                                                                                                              ggcgAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTa                                   >  4:376905/1‑149 (MQ=255)
                                                                                                                    ggTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGaa                             <  3:92972/149‑1 (MQ=255)
                                                                                                                    ggTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGaa                             <  6:182629/149‑1 (MQ=255)
                                                                                                                      tGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAggggg                                       <  6:138386/137‑1 (MQ=255)
                                                                                                                      tGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAggggg                                       >  5:138386/1‑137 (MQ=255)
                                                                                                                         ggTCCTTGTCGCTTAACTGGCGAAACAGGTGTTGCGACTCGAAGTCCGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGAAAAACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGAAGAGc                         >  5:327126/1‑148 (MQ=255)
                                                                                                                         ggTCCTTGTCACTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTAGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGAAGTGcg                        <  2:332707/149‑1 (MQ=255)
                                                                                                                                 tCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGAAGTGcgatccgatc                <  8:371365/149‑1 (MQ=255)
                                                                                                                                        aCTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGAAGTGCGATCCGATCTTCGATg         <  3:359614/149‑1 (MQ=255)
                                                                                                                                         cTGGCGCACCAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGAAGTGCGATCCGATCTTCGATGc        <  4:281975/149‑1 (MQ=255)
                                                                                                                                          tGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGTGCTTCGGCCGCGCTAAGGCGGCTTATTTGAAGTGCGATCCGATCTTCGATGCg       <  5:278693/149‑1 (MQ=255)
                                                                                                                                          tGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGAAGTGCGATCCGATCTTCGATGCg       >  1:102690/1‑149 (MQ=255)
                                                                                                                                           gtcgcAACACCTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGAAGTGCGATCCGATCTTCGATGCgg      >  4:353126/3‑149 (MQ=255)
                                                                                                                                            tcgcAACACCTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCACCACATCTGGTTCAAGGGCTTAGGCCGCGCTAAGTGGGCTTATTTGAAGTGCGATCAGATCTTCGATGCgg      >  1:192714/2‑148 (MQ=255)
                                                                                                                                            gcgcAACAGGTGTTGCGACTCGAAGGAGTCCAGTTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGAAGTGCGATCCGATCTTCGATGCGgt     <  7:168208/149‑1 (MQ=255)
                                                                                                                                             cgcAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGAAGTGCGATCCGATCTTCGATGCGgta    <  7:52604/149‑1 (MQ=255)
                                                                                                                                              gcAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGAAGTGCGATCCGATCTTCGATGCGgtag   <  4:397857/149‑1 (MQ=255)
                                                                                                                                               cAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGAAGTGCGATCCGATCTTCGATGCGgtagt  <  5:210299/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CCGCCTTCGGAAATGACCACCGGCAGCGGCTCGCCGACTTCGCCAAGCAGGGTTTGCCCAACCACCGCGCTGAGCAAGTCGAGGCGCTTGTTCTGTGCCCGCAGGAAGGCGGCGAGGGTGCGGTCCTTGTCGCTTAACTGGCGCAACAGGTGTTGCGACTCGAAGTCGGCCAGGTGCAGCTCGCTGAGCAGGTTGAACAGCGGCGAATCATCTTGCAACACATCTGGTTCAAGGGCTTCGGCCGCGCTAAGGGGGCTTATTTGAAGTGCGATCCGATCTTCGATGCGGTAGT  >  NC_002947/2460583‑2460874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑