Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,474,155	G→A	24.9%	intergenic (‑60/+120)	PP_2167 ← / ← tal	PhoD‑like phosphatase/transaldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,474,155	0	G	A	24.9%	61.6 / 16.2	37	intergenic (‑60/+120)	PP_2167/tal	PhoD‑like phosphatase/transaldolase
Reads supporting (aligned to +/- strand):  ref base G (15/12);  new base A (5/4);  total (21/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

TGACGAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAAC  >  NC_002947/2474008‑2474296                                                                                                                                                    |                                                                                                                                              tGACGAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAgt                                                                                                                                              <  5:299675/149‑1 (MQ=255)           gacggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCgg                                                                                                                                    <  6:339207/149‑1 (MQ=255)             cggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGTTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAGGGCggg                                                                                                                                   <  4:197276/148‑1 (MQ=37)                 cagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGTGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCTCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGc                                                                                                                              <  5:133697/149‑1 (MQ=255)                 cagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAGGGCGGGCTTGc                                                                                                                              <  6:349612/149‑1 (MQ=37)                   gacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGccc                                                                                                                            <  7:101034/149‑1 (MQ=255)                        gggggTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTGGCCCGCGaa                                                                                                                       <  5:321776/148‑1 (MQ=255)                         ggggTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCg                                                                                                                                     >  3:151697/1‑134 (MQ=255)                         ggggTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCg                                                                                                                                     <  4:151697/134‑1 (MQ=255)                             ttCCAGGCGGCGCAGAACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGCTGGCTATGGCGGCCTATTCGCGGCCAAGCCAGCCCTTACATCGATCAGTGTAGGACCGGGCTTGCCCGCGAACAGGc                                                                                                                  >  2:281546/1‑149 (MQ=255)                                    cggcgAAGTACCGGGCCTGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAGGGCGGGCTTGCCcg                                                                                                                           <  8:350776/133‑1 (MQ=21)                                     ggcgCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCtt                                                                                                                                <  3:212624/127‑1 (MQ=255)                                     ggcgCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCtt                                                                                                                                >  4:212624/1‑127 (MQ=255)                                                          gaACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAGGGCGGGCTTGc                                                                                                                              >  2:259522/1‑108 (MQ=18)                                                          gaACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAGGGCGGGCTTGc                                                                                                                              <  1:259522/108‑1 (MQ=18)                                                           aaCGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAgtg                                                                                                                                             <  3:254806/92‑1 (MQ=255)                                                           aaCGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAgtg                                                                                                                                             >  4:254806/1‑92 (MQ=255)                                                                ggggCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAAtt                                                                                                         <  3:233932/123‑1 (MQ=255)                                                                ggggCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCAGTTAGCGGGCCAGCCCCCCCATACATCGATCACTTTAGGAGCGGGCGTGGCCCCGAAACGGCCCCACAAtt                                                                                                         >  4:233932/1‑123 (MQ=255)                                                                                            cAGCTGGGGTGGGTTTGGCGGCCTGTGCGCGGGCATGCCCGCCCTTACATCGATCAGTTTAGCTGGGGGCTTGCCCGCGAACAGGCCCGAGATTTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTc                                                   <  4:379436/149‑1 (MQ=255)                                                                                                                    gTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACt                           >  5:174146/1‑149 (MQ=255)                                                                                                                        gcgGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTa                        >  3:377581/1‑148 (MQ=255)                                                                                                                              aaGCCCGCTCCTACACTGATCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGcc                 >  2:2524/1‑149 (MQ=37)                                                                                                                                gcccgctcCTACACTGATCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCtt               >  1:153701/1‑149 (MQ=37)                                                                                                                                gcccgctcCTACACTGATCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCtt               >  1:159419/1‑149 (MQ=37)                                                                                                                                 cccgcccTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCttt              >  5:260410/1‑149 (MQ=255)                                                                                                                                  ccgctcCTACACTGATCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGACAGGCAAACACGAACTGTATCAGGCCttt              >  7:89724/1‑148 (MQ=37)                                                                                                                                  ccgcccTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTa                        <  5:382472/138‑1 (MQ=255)                                                                                                                                  ccgcccTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTa                        >  6:382472/1‑138 (MQ=255)                                                                                                                                  ccgcccTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCttt              >  4:228020/1‑148 (MQ=255)                                                                                                                                    gcccTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCtttt             >  5:46425/1‑147 (MQ=255)                                                                                                                                        ttACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTa       >  5:182110/1‑149 (MQ=255)                                                                                                                                        ttACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTa       >  5:294231/1‑149 (MQ=255)                                                                                                                                        ttACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTa       >  8:159181/1‑149 (MQ=255)                                                                                                                                           cATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCTGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATc     <  4:377581/148‑1 (MQ=255)                                                                                                                                           cATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATc     >  7:326876/1‑148 (MQ=255)                                                                                                                                           cATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATc     >  7:77931/1‑148 (MQ=255)                                                                                                                                           cATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATc     <  3:228020/148‑1 (MQ=255)                                                                                                                                            aTCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCaa   >  1:306717/1‑149 (MQ=255)                                                                                                                                             tCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAAc  <  6:294231/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              TGACGAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAAC  >  NC_002947/2474008‑2474296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑