Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,633,457	T→G	6.5%	intergenic (+100/‑82)	hupB → / → PP_2304	DNA binding regulator subunit beta B/peptidyl‑prolyl cis‑trans isomerase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,633,457	0	T	G	6.5%	187.1 / 4.6	62	intergenic (+100/‑82)	hupB/PP_2304	DNA binding regulator subunit beta B/peptidyl‑prolyl cis‑trans isomerase D
Reads supporting (aligned to +/- strand):  ref base T (26/32);  new base G (2/2);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

GTTCCAGGTTTCAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATCGTC  >  NC_002947/2633310‑2633604                                                                                                                                                    |                                                                                                                                                        gTTCCAGGTGTCAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCAACCTc                                                                                                                                                        <  8:335223/149‑1 (MQ=255)      aGGTTTCAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCATTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATg                                                                                                                                                   >  7:277621/1‑149 (MQ=255)            cAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCc‑t                                                                                                                                             <  3:118092/148‑1 (MQ=255)                  cGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCtttt                                                                                                                                      <  5:322798/149‑1 (MQ=255)                  cGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCGAGGATGCGCc‑ttttctta                                                                                                                                      <  5:146419/149‑6 (MQ=255)                    gCAAAGGACTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGTATGCGCC‑TTTCTTTTAt                                                                                                                                    <  4:91855/149‑1 (MQ=255)                     cAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTAt                                                                                                                                    >  4:55920/1‑148 (MQ=255)                         ggCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGGACTACGATAGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGa                                                                                                                               <  1:131459/149‑1 (MQ=255)                           ccTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGAtt                                                                                                                             <  1:43908/149‑1 (MQ=255)                                         tCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCcg                                                                                                               >  5:406401/1‑149 (MQ=255)                                           aaCTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCcgc                                                                                                              <  1:1398/148‑1 (MQ=255)                                             cTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGt                                                                                                           >  4:390875/1‑149 (MQ=255)                                             cTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGt                                                                                                           >  2:18061/1‑149 (MQ=255)                                                 gtcgtcTTTCAGCCGGACCCTGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTc                                                                                                       <  2:406869/149‑1 (MQ=255)                                                 gtcgtcTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTc                                                                                                       <  5:376496/149‑1 (MQ=255)                                                 gtcgtcTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTc                                                                                                       >  4:307485/1‑149 (MQ=255)                                                  tcgtcTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCGGGATTCTACCCACGCTCCGCGGTTGTc                                                                                                       >  1:49129/1‑148 (MQ=255)                                                    ctggttCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGa                                                                                                     <  1:241915/144‑1 (MQ=255)                                                          cAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTg                                                                                              >  2:136445/1‑149 (MQ=255)                                                           aGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCGCCTCGGTTGTCGACGCAGTgg                                                                                             >  6:284613/1‑149 (MQ=255)                                                            gCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TGTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGt                                                                                            >  5:247510/1‑149 (MQ=255)                                                               ggACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACa                                                                                         <  3:296853/149‑1 (MQ=255)                                                               ggACCAGGCCAGGCCGGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCgg                                                                                                            <  3:268708/130‑1 (MQ=255)                                                               ggACCAGGCCAGGCCGGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCgg                                                                                                            >  4:268708/1‑130 (MQ=255)                                                                gACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAG‑AAAGGCGCATCCGAGGATGCGCc‑ttttctta                                                                                                                                      <  7:227805/102‑6 (MQ=37)                                                                gACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAG‑AAAGGCGCATCCGAGGATGCGCc‑ttttctta                                                                                                                                      >  8:227805/1‑97 (MQ=37)                                                                   ccGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCg                                                                                     >  4:183303/1‑149 (MQ=255)                                                                           gcctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTggg                                                                          <  3:55920/149‑1 (MQ=255)                                                                           gcctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTggg                                                                          <  4:138824/149‑1 (MQ=255)                                                                           gcctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTg                                                                            <  3:390875/147‑1 (MQ=255)                                                                           gcctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTg                                                                            <  1:375062/147‑1 (MQ=255)                                                                           gcctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTg                                                                            <  2:289098/147‑1 (MQ=255)                                                                             ctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACCCGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCACTGGTACAGCCG‑‑‑TTTCTggggg                                                                        >  5:171283/1‑149 (MQ=255)                                                                                gTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCcgcg                                                                                                             >  5:284309/1‑112 (MQ=255)                                                                                gTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCcgcg                                                                                                             <  6:284309/112‑1 (MQ=255)                                                                                 tCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACg                                                                     >  6:161206/1‑148 (MQ=255)                                                                                       cACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCAtgct                                                               <  4:245783/148‑1 (MQ=255)                                                                                                 gCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCa                                                    <  3:307485/149‑1 (MQ=255)                                                                                                 gCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATCCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATc                                                     <  6:285611/148‑1 (MQ=255)                                                                                                    gggCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCAtg                                                                 <  2:152544/133‑1 (MQ=255)                                                                                                    gggCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCAtg                                                                 >  1:152544/1‑133 (MQ=255)                                                                                                        gcAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACaa                                             >  8:97200/1‑149 (MQ=255)                                                                                                           aaCGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCTTCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTc                                          <  8:277621/149‑1 (MQ=255)                                                                                                                  ccGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGt                                   >  6:132772/1‑149 (MQ=255)                                                                                                                  ccGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGt                                   >  8:276175/1‑149 (MQ=255)                                                                                                                   cGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGtt                                  <  6:406401/149‑1 (MQ=255)                                                                                                                    gCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTg                                 <  7:97200/149‑1 (MQ=255)                                                                                                                     ccTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACTGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTgg                                <  1:105132/149‑1 (MQ=255)                                                                                                                         acacGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCAGAGAGTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGa                               >  1:334732/1‑149 (MQ=255)                                                                                                                              tttGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGcc                          >  1:347649/1‑146 (MQ=255)                                                                                                                                   tAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAg                                                                                        <  5:391643/82‑1 (MQ=255)                                                                                                                                   tAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAg                                                                                        >  6:391643/1‑82 (MQ=255)                                                                                                                                   tAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAgg                                                  <  6:112096/117‑1 (MQ=255)                                                                                                                                   tAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAgg                                                  >  5:112096/1‑117 (MQ=255)                                                                                                                                   aaaagaaaGGCGCATCCGAGGATGCGCCTTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACca                   >  7:13415/6‑149 (MQ=39)                                                                                                                                     aGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTTGATTGCCCAGACcatca                >  8:158040/1‑149 (MQ=255)                                                                                                                                        aaaGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCg             <  5:132772/149‑1 (MQ=255)                                                                                                                                              gcATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTa       >  1:399923/1‑149 (MQ=255)                                                                                                                                                aTCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTg                            >  7:287631/1‑126 (MQ=255)                                                                                                                                                aTCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTg                            <  8:287631/126‑1 (MQ=255)                                                                                                                                                   cTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTAtcgtc  <  2:399923/149‑1 (MQ=255)                                                                                                                                                   cTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTAtcgt   <  3:183303/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                        GTTCCAGGTTTCAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCG‑‑‑TTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATCGTC  >  NC_002947/2633310‑2633604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑