Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,644,841 G→C 5.3% P825A (CCG→GCG) ‡ PP_2316 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,644,8410GC 5.3% 248.4 / 3.4 76P825A (CCG→GCG) ‡PP_2316ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (33/39);  new base C (2/2);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01

GTGAAGCGACCTGGTATGTGAGCAGCATCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCATAGCGGTACAGCACCCAGGTAATCAGTTCGCA  >  NC_002947/2644693‑2644988
                                                                                                                                                    |                                                                                                                                                   
gTGAAGCGACCTGGTATGTGAGCAGCATCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCgg                                                                                                                                                     >  4:278777/1‑149 (MQ=255)
     gCGACCTGGTATGTGAGCAGCATCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTgg                                                                                                                                                >  5:226399/1‑149 (MQ=255)
     gCGACCTGGTATGTGAGCAGCATCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCCCGCTgg                                                                                                                                                >  3:322786/1‑149 (MQ=255)
        aCCTGGTATGTGAGCAGCATCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCAt                                                                                                                                             >  3:43406/1‑149 (MQ=255)
        aCCTGGTATGTGAGCAGCATCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCCCGCTGGcgg                                                                                                                                             <  4:322786/149‑3 (MQ=255)
         ccTGGTATGTGAGCAGCATCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCAtt                                                                                                                                            <  8:319019/149‑1 (MQ=255)
          cTGGTATGTGAGCAGCATCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCAtt                                                                                                                                            >  8:45373/1‑148 (MQ=255)
             gTATGTGAGCAGCATCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCaa                                                                                                                                         <  8:126999/148‑1 (MQ=255)
                tgtgAGCAGCATCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAAcgc                                                                                                                                      <  7:45373/148‑1 (MQ=255)
                  tgAGCAGCATCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAAcgcccg                                                                                                                                   >  8:72869/1‑149 (MQ=255)
                   gAGCAGCATCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAAcgcccgc                                                                                                                                  >  5:207759/1‑149 (MQ=255)
                   gAGCAGCATCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAAcgcccgc                                                                                                                                  >  3:52438/1‑149 (MQ=255)
                       agcaTCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCgtg                                                                                                                              <  4:43406/149‑1 (MQ=255)
                        gcaTCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCgtgt                                                                                                                             <  1:332883/149‑1 (MQ=255)
                          aTCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTc                                                                                                                           >  8:57525/1‑149 (MQ=255)
                           tCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGc                                                                                                                                                   >  5:256462/1‑124 (MQ=255)
                           tCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGc                                                                                                                                                   <  6:256462/124‑1 (MQ=255)
                           tCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTcc                                                                                                                          >  3:24955/1‑149 (MQ=255)
                           tCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTcc                                                                                                                          >  7:26900/1‑149 (MQ=255)
                           tCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTcc                                                                                                                          >  4:161342/1‑149 (MQ=255)
                            ccGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAAc                                                                                                                                        >  6:370722/1‑134 (MQ=255)
                            ccGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAAc                                                                                                                                        <  5:370722/134‑1 (MQ=255)
                             cGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTccca                                                                                                                        <  7:113462/149‑1 (MQ=255)
                             cGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTccca                                                                                                                        <  6:226399/149‑1 (MQ=255)
                             cGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTccca                                                                                                                        <  4:295925/149‑1 (MQ=255)
                             cGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTccc                                                                                                                         <  3:278777/148‑1 (MQ=255)
                              gTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTcccag                                                                                                                       <  4:293962/149‑1 (MQ=255)
                                  cGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTcccagcac                                                                                                                    <  8:26900/148‑1 (MQ=255)
                                   ggCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCCCGCTGGcgg                                                                                                                                             <  5:182210/122‑3 (MQ=255)
                                   ggCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCCCGCTGGcgg                                                                                                                                             >  6:182210/1‑120 (MQ=255)
                                    gCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTcccagcaccc                                                                                                                  >  5:9228/1‑148 (MQ=255)
                                     ccATGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTcccagcacccca                                                                                                                <  3:124118/149‑1 (MQ=255)
                                     ccAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTcccagcaccc                                                                                                                  >  1:393257/1‑147 (MQ=255)
                                          gCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGTGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTcc                                                                                                                          <  6:394016/134‑1 (MQ=255)
                                          gCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGCGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTcc                                                                                                                          >  5:394016/1‑134 (MQ=255)
                                             tctcGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCaccacc                                                                                                        <  7:72869/149‑1 (MQ=255)
                                               tcGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCCGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCCGCACCCCAGCAccaccca                                                                                                      >  5:28448/1‑149 (MQ=255)
                                                 gACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCAccacccac                                                                                                     >  6:400910/1‑148 (MQ=255)
                                                 gACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCAccacccac                                                                                                     <  6:11040/148‑1 (MQ=255)
                                                  aCGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACccacca                                                                                                   <  4:70994/149‑1 (MQ=255)
                                                    ggACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACcag                                                                                                  >  2:1733/1‑148 (MQ=255)
                                                     gactgactGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACcagca                                                                                                <  3:203354/149‑1 (MQ=255)
                                                        tgactgGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTg                                                                                             >  1:62420/1‑149 (MQ=255)
                                                          actgGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGcc                                                                                           >  7:18283/1‑149 (MQ=255)
                                                          actgGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGc                                                                                            <  5:400910/148‑1 (MQ=255)
                                                              gCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGccccc                                                                                        <  4:52438/148‑1 (MQ=255)
                                                                   tCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGc                                                                                  <  1:57955/149‑1 (MQ=255)
                                                                    cTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAg                                                                                   >  5:383625/1‑147 (MQ=255)
                                                                    cTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAg                                                                                   <  6:383625/147‑1 (MQ=255)
                                                                       gCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGc                                                                               >  2:64908/1‑148 (MQ=255)
                                                                            aGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAacac                                                                          <  6:9228/148‑1 (MQ=255)
                                                                                 gAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTccca                                                                                                                        <  2:347761/97‑1 (MQ=255)
                                                                                 gAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTccca                                                                                                                        >  1:347761/1‑97 (MQ=255)
                                                                                     cATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCAc                                                                <  4:24955/149‑1 (MQ=255)
                                                                                         tgCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGt                                                            <  3:161342/149‑1 (MQ=255)
                                                                                         tgCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGt                                                            <  1:1733/149‑1 (MQ=255)
                                                                                            tGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTg                                                                                                                                                 >  5:262711/1‑61 (MQ=255)
                                                                                            tGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTg                                                                                                                                                 <  6:262711/61‑1 (MQ=255)
                                                                                                ccGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCt                                                     >  6:385456/1‑149 (MQ=255)
                                                                                                 cGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACcagcag                                                                    <  3:267325/133‑1 (MQ=255)
                                                                                                 cGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCAACACCACTGCCCCCCTCAGCGGCAACACcagcag                                                                    >  4:267325/1‑133 (MQ=255)
                                                                                                     aGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACt                                                >  8:305005/1‑149 (MQ=255)
                                                                                                        aGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGc                                              <  6:207759/148‑1 (MQ=255)
                                                                                                              gACTGCCAGGCCTCAAGCCGCGCTCAACACCGCCCGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGa                                       <  8:305162/149‑1 (MQ=255)
                                                                                                                 tGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAgg                                    >  6:59653/1‑149 (MQ=255)
                                                                                                                     aGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCa                                  >  8:99092/1‑147 (MQ=255)
                                                                                                                      ggCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCATAg                               <  7:305005/149‑1 (MQ=255)
                                                                                                                       gCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGAGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCATAGc                              <  5:61073/149‑1 (MQ=255)
                                                                                                                         cTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCATAGAgg                            >  4:274546/1‑149 (MQ=255)
                                                                                                                          tCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCATAGCgg                            >  7:314489/1‑148 (MQ=255)
                                                                                                                          tCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCATAGCgg                            >  6:198639/1‑148 (MQ=255)
                                                                                                                          tCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCATAGCg                             >  4:352609/1‑147 (MQ=255)
                                                                                                                          tCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCATAGCg                             <  3:352609/147‑1 (MQ=255)
                                                                                                                                     gcAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCATAGCGGTACAGCACCCAg                <  2:62420/149‑1 (MQ=255)
                                                                                                                                     gcAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCATAGCGGTACAGCACCCAg                <  7:57525/149‑1 (MQ=255)
                                                                                                                                     gcAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCATAGCGGTACAGCACCCAg                <  2:393257/149‑1 (MQ=255)
                                                                                                                                               cAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCATAGCGGTACAGCACCCAGGTAATCAGtt      <  5:59653/149‑1 (MQ=255)
                                                                                                                                                   gggCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCATAGCGGTACAGCACCCAGGTAATCAGTTCGCa  <  1:64908/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTGAAGCGACCTGGTATGTGAGCAGCATCCGTTACGGCCAGGGCGTCTCGACGGACTGACTGGCCCGTCTTGCGTCAGCTTGAGGCATGTGCTGTCCCGGCAGAAGCGCTGACTGCCAGGCCTCAAGCCTCGCGCAACACCGCCAGCGGGCTGGCATTCAACGCCCGCCGTGTTCCCAGCACCCCAGCACCACCCACCAGCAGTGCCCCCGTCAGCGGCAACACCAGCAGCCACGGGTGCGGGCTCCACTGCAAATCGAAGGCATAGCGGTACAGCACCCAGGTAATCAGTTCGCA  >  NC_002947/2644693‑2644988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: