Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,659,276 T→C 5.3% T354A (ACC→GCC)  PP_2331 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,659,2760TC 5.3% 223.7 / 2.1 75T354A (ACC→GCC) PP_2331membrane protein
Reads supporting (aligned to +/- strand):  ref base T (41/29);  new base C (2/2);  total (43/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

GCGGCAATCAGCACCACGACGAAGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCG  >  NC_002947/2659134‑2659424
                                                                                                                                              |                                                                                                                                                    
gcggcAATCAGCACCACGACGAAGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTcgc                                                                                                                                                 <  3:213425/148‑1 (MQ=255)
gcggcAATCAGCACCACGACGAAGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAACCCAGCTGGGCTTcgg                                                                                                                                                 <  5:394579/148‑2 (MQ=255)
 cggcAATCAGCACCACGACGAAGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTcgcg                                                                                                                                                >  3:226477/1‑148 (MQ=255)
      aTCAGCACCACGACGAAGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAACCCAGCTGGGCTTcg                                                                                                                                                  >  5:289094/1‑141 (MQ=255)
      aTCAGCACCACGACGAAGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAACCCAGCTGGGCTTcg                                                                                                                                                  <  6:289094/141‑1 (MQ=255)
       tCAGCACCACGACGAAGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTcgc                                                                                                                                                 >  1:162900/1‑141 (MQ=255)
         aGCACCACGACGAAGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCa                                                                                                                                        <  8:48496/148‑1 (MQ=255)
          gCACCACGACGAAGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAgg                                                                                                                                      >  7:126058/1‑149 (MQ=255)
              cacGACGAAGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGa                                                                                                                                  >  1:206842/1‑149 (MQ=255)
              cacGACGAAGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGa                                                                                                                                  >  2:84300/1‑149 (MQ=255)
               acgacgAAGGTCAATACCACCCACAGCCGTGGCAGCAGCTCCAGCTTGCGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGAGCACCGTTACCAGCATGATACCGAAGCCCAGCTGGGGTTCGCGGAACGCCAGGGCGAt                                                                                                                                 <  8:288225/149‑1 (MQ=255)
                 gacgaAGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGAt                                                                                                                                 >  5:32557/1‑147 (MQ=255)
                   cgaAGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAg                                                                                                                              >  2:292256/1‑148 (MQ=255)
                      aGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCAcc                                                                                                                          >  5:75251/1‑149 (MQ=255)
                        gTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCgg                                                                                                                        >  2:142378/1‑149 (MQ=255)
                         tCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCggg                                                                                                                       >  3:308795/1‑149 (MQ=255)
                         tCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCggg                                                                                                                       >  2:342672/1‑149 (MQ=255)
                         tCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCggg                                                                                                                       <  1:84300/149‑1 (MQ=255)
                              accaccGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGaa                                                                                                                  <  3:261043/149‑1 (MQ=255)
                               ccaccGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGaa                                                                                                                  <  4:226477/148‑1 (MQ=255)
                               ccaccGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAAt                                                                                                                 <  6:32557/149‑1 (MQ=255)
                                caccGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAAtg                                                                                                                >  5:186271/1‑149 (MQ=255)
                                      cAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCa                                                                                                          <  4:193438/149‑1 (MQ=255)
                                       aGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAg                                                                                                         <  6:186271/149‑1 (MQ=255)
                                       aGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAg                                                                                                         <  8:126058/149‑1 (MQ=255)
                                         gCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAggg                                                                                                       >  4:383352/1‑149 (MQ=255)
                                            tGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCt                                                                                                    >  2:112905/1‑149 (MQ=255)
                                              gcagcaTCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAg                                                                                                         >  3:169469/1‑142 (MQ=255)
                                              gcagcaTCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAg                                                                                                         <  4:169469/142‑1 (MQ=255)
                                                agcaTCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATGCCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGAt                                                                                                <  4:100624/149‑1 (MQ=255)
                                                 gcaTCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATg                                                                                               <  1:292256/149‑1 (MQ=255)
                                                  caTCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGc                                                                                              <  1:142378/149‑1 (MQ=255)
                                                    tCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGcc                                                                                             >  2:383549/1‑148 (MQ=255)
                                                        cAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGa                                                                                           <  2:206842/146‑1 (MQ=255)
                                                                    ttCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGTTCAGGTTGCgca                                                                               >  1:343164/1‑146 (MQ=255)
                                                                    ttCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCgcagca                                                                            >  4:12617/1‑149 (MQ=255)
                                                                    ttCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCgcagca                                                                            >  6:386154/1‑149 (MQ=255)
                                                                    ttCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCgca                                                                               <  2:343164/146‑1 (MQ=255)
                                                                      ccAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCAc                                                                           <  6:75251/148‑1 (MQ=255)
                                                                       cAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCAc                                                                           >  3:365891/1‑147 (MQ=255)
                                                                        aGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCGAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCAc                                                                           <  4:246919/146‑1 (MQ=255)
                                                                        aGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCAc                                                                           >  3:246919/1‑146 (MQ=255)
                                                                        aGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAg                                                                        <  2:372980/149‑1 (MQ=255)
                                                                          gTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCa                                                                         <  3:77613/146‑1 (MQ=255)
                                                                          gTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCa                                                                         >  4:77613/1‑146 (MQ=255)
                                                                           tACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGAt                                                                     >  6:235151/1‑149 (MQ=255)
                                                                           tACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGAt                                                                     <  5:386154/149‑1 (MQ=255)
                                                                             cGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATc                                                                    <  1:112905/148‑1 (MQ=255)
                                                                                gcgcAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAgg                                                                                                        >  6:18812/1‑109 (MQ=255)
                                                                                gcgcAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAgg                                                                                                        <  5:18812/109‑1 (MQ=255)
                                                                                  gcAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAg                                                               >  3:76336/1‑148 (MQ=255)
                                                                                    aGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGCGTTTCGCGGAACGCCAGGGCGATCAGCTCCGGGGTGAATGTGCCCAGTGCCTGGATGCCGCTCAGGTTGCGCAGCACCACGATAAACAGCAc                                                            >  7:149106/1‑149 (MQ=255)
                                                                                          aGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGAt                                                      >  4:403037/1‑149 (MQ=255)
                                                                                            gCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCg                                                    >  4:50519/1‑149 (MQ=255)
                                                                                                       tgATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGAc                                          <  4:365891/148‑1 (MQ=255)
                                                                                                       tgATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGACCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGAc                                          >  7:227790/1‑148 (MQ=255)
                                                                                                         aTCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAgg                                                                                                                                      >  5:107367/1‑54 (MQ=255)
                                                                                                         aTCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAgg                                                                                                                                      <  6:107367/54‑1 (MQ=255)
                                                                                                         aTCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAgg                                                                       >  8:80857/1‑117 (MQ=255)
                                                                                                         aTCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAgg                                                                       <  7:80857/117‑1 (MQ=255)
                                                                                                         aTCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCAt                                       >  7:30137/1‑149 (MQ=255)
                                                                                                              cGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCa                                  <  3:12617/149‑1 (MQ=255)
                                                                                                               gTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTCGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCAGATCGGGATCATGACCATGATCAt                                 >  7:5782/1‑149 (MQ=255)
                                                                                                                 gAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATg                                >  2:2702/1‑148 (MQ=255)
                                                                                                                    cAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAgg                            >  4:44329/1‑149 (MQ=255)
                                                                                                                          gATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCt                      >  7:19862/1‑149 (MQ=255)
                                                                                                                             aCCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTggg                   >  4:131321/1‑149 (MQ=255)
                                                                                                                              gcGAAACCCAGCTGGGCTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGt                  >  1:47642/2‑149 (MQ=255)
                                                                                                                               cGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGtt                 >  5:88362/1‑149 (MQ=255)
                                                                                                                               cGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGtt                 <  1:342672/149‑1 (MQ=255)
                                                                                                                               cGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGtt                 >  5:161040/1‑149 (MQ=255)
                                                                                                                                    cccAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAg            >  3:119385/1‑149 (MQ=255)
                                                                                                                                     ccAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGt           <  3:383352/149‑1 (MQ=255)
                                                                                                                                       aGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTgg         >  4:402180/1‑149 (MQ=255)
                                                                                                                                            ggTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGc    <  1:2702/149‑1 (MQ=255)
                                                                                                                                              tttCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCg  >  2:284646/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GCGGCAATCAGCACCACGACGAAGGTCAATACCACCGACAGGCGTGGCAGCATCTGCAGCTTGAGGTGTTCCAGGTACGAGCGCAGCGACAGGCCCAGTGCTGTGATCACCGTGAACAGCATGATACCGAAGCCCAGCTGGGTTTCGCGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCG  >  NC_002947/2659134‑2659424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑