Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,669,671	T→G	9.7%	intergenic (+38/+37)	prpD → / ← acnB	2‑methylcitrate dehydratase/aconitate hydratase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,669,671	0	T	G	9.7%	181.8 / 9.8	62	intergenic (+38/+37)	prpD/acnB	2‑methylcitrate dehydratase/aconitate hydratase B
Reads supporting (aligned to +/- strand):  ref base T (32/24);  new base G (2/4);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

GACCCGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGATGTCTTTC  >  NC_002947/2669525‑2669819                                                                                                                                                      |                                                                                                                                                     gACCCGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCg                                                                                                                                                    >  2:396600/1‑149 (MQ=255) gACCCGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCg                                                                                                                                                    >  7:150400/1‑149 (MQ=255)    ccGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTg                                                                                                                                                 >  7:330562/1‑149 (MQ=255)       cTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCg                                                                                                                                              >  5:228257/1‑149 (MQ=255)             ccGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCAGCGATGCCGGGGCtt                                                                                                                                        <  4:112821/149‑1 (MQ=255)              cGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCttt                                                                                                                                       >  5:373687/1‑149 (MQ=255)              cGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCAGCGATGCCGGGGCttt                                                                                                                                       <  7:47815/149‑1 (MQ=255)                 cAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGtt                                                                                                                                    <  8:150400/149‑1 (MQ=255)                  aGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGttt                                                                                                                                   <  6:373687/149‑1 (MQ=255)                  aGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGttt                                                                                                                                   <  5:63838/149‑1 (MQ=255)                      gtgtCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACaa                                                                                                                               <  5:324667/149‑1 (MQ=255)                       tgtCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAAc                                                                                                                              <  8:17638/149‑1 (MQ=255)                         tCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCCCTCTTAGTGTGCACAGGTTCGTGTACTTGTTGGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACa                                                                                                                                <  1:335163/148‑1 (MQ=255)                           agcagGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTg                                                                                                                           >  2:112715/1‑148 (MQ=255)                              agGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTa                                                                                                                                  <  3:368199/138‑1 (MQ=255)                              agGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCCGCATCGCTGCCGGGGCTTTGTTTa                                                                                                                                  >  4:368199/1‑138 (MQ=255)                                gTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTc                                                                                                                     <  6:228257/149‑1 (MQ=255)                                   ttCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAg                                                                                                                   <  2:148177/148‑1 (MQ=255)                                    tCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAAc                                                                                                                              <  3:303541/136‑1 (MQ=255)                                    tCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAAc                                                                                                                              >  4:303541/1‑136 (MQ=255)                                    tCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGc                                                                                                                  <  4:192968/148‑1 (MQ=255)                                     cGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCt                                                                                                                 >  2:45857/1‑148 (MQ=255)                                     cGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCt                                                                                                                 <  8:280538/148‑1 (MQ=255)                                      gAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGc                                                                                                                                          >  7:61575/1‑122 (MQ=255)                                      gAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGc                                                                                                                                          <  8:61575/122‑1 (MQ=255)                                              cAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGaa                                                                                                       <  1:396600/149‑1 (MQ=255)                                                     cagcagAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCgg                                                                                                <  8:330562/149‑1 (MQ=255)                                                      agcagAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGATCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTATGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCgg                                                                                                >  1:122827/1‑148 (MQ=255)                                                           aaGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTTGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCtt                                                                                          <  3:103382/149‑1 (MQ=255)                                                            aGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCACCTTACGCCTGAACCACCGGGATCTTg                                                                                         <  1:216778/149‑1 (MQ=255)                                                                 gAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGtt                                                                                    >  3:170897/1‑149 (MQ=255)                                                                       aC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCAGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGc                                                                              >  2:336838/1‑149 (MQ=255)                                                                            ggCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCg                                                                            <  3:275961/149‑1 (MQ=255)                                                                                        gTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACt                                                                >  6:234382/1‑149 (MQ=255)                                                                                               gACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGAt                                                         >  8:242244/1‑149 (MQ=255)                                                                                                     tttgtGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATc                                                        <  4:383803/144‑1 (MQ=255)                                                                                                     tttgtGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATc                                                        >  3:383803/1‑144 (MQ=255)                                                                                                     tttgtGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGt                                                    <  1:45857/148‑1 (MQ=255)                                                                                                               gAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAgg                                         >  1:67636/1‑149 (MQ=255)                                                                                                               gAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCCCAgg                                         >  1:409099/1‑149 (MQ=255)                                                                                                                 aGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAgg                                         >  1:156212/1‑147 (MQ=255)                                                                                                                             aGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTc                           >  8:334919/1‑149 (MQ=255)                                                                                                                             aGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACATc                           >  1:163104/1‑149 (MQ=255)                                                                                                                             aGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACCCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTc                           >  5:408950/1‑149 (MQ=255)                                                                                                                               gCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCTCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCgg                         >  8:194555/1‑149 (MQ=255)                                                                                                                               gCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCgg                         >  3:328091/1‑149 (MQ=255)                                                                                                                               gCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCgg                         >  7:386005/1‑149 (MQ=255)                                                                                                                                   aaaCAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCAAAGCTCAGGTAGCGGTAAACGTCGGCAGc                     >  2:111632/1‑149 (MQ=255)                                                                                                                                       aacaaAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCTGCGATCTGGTCGAAGCTCATGTAGCGGTAAACGTCGGCAGCCATg                 <  2:122827/149‑1 (MQ=255)                                                                                                                                       aacaaAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTATCGGTAAACGTAGGCAGCCAt                  >  6:285407/1‑148 (MQ=255)                                                                                                                                         caaAGCCCCGGCAGCGATGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCt               >  5:264609/1‑149 (MQ=255)                                                                                                                                          aaaGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTg              >  7:249778/1‑149 (MQ=255)                                                                                                                                          aaaGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTg              >  2:298022/1‑149 (MQ=255)                                                                                                                                          aaaGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTg              >  4:107351/1‑149 (MQ=255)                                                                                                                                           aaGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGt             <  2:156212/149‑1 (MQ=255)                                                                                                                                            aGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTg              >  2:365282/1‑147 (MQ=255)                                                                                                                                              ccccGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGa          <  7:242244/149‑1 (MQ=255)                                                                                                                                              ccccGGCAGCGATGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGa          <  6:264609/149‑1 (MQ=255)                                                                                                                                               cccGGCAGCGATGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTc            <  8:298675/146‑1 (MQ=255)                                                                                                                                               cccGGCAGCGATGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTc            >  7:298675/1‑146 (MQ=255)                                                                                                                                                 cGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCCCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGATGt       <  2:409099/149‑1 (MQ=255)                                                                                                                                                   gCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGATGTCt     >  4:146520/1‑149 (MQ=255)                                                                                                                                                      tCGCTGCCGGTGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGATGTCTTTc  <  3:107351/149‑1 (MQ=255)                                                                                                                                                      tCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGATGTCTTTc  <  5:234382/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     GACCCGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCAC‑‑‑GGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGATGTCTTTC  >  NC_002947/2669525‑2669819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑