Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,721,400 T→A 8.7% intergenic (‑362/‑80) PP_2381 ← / → PP_2382 hypothetical protein/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,721,4000TA 8.7% 131.7 / 3.5 46intergenic (‑362/‑80)PP_2381/PP_2382hypothetical protein/putative transporter
Reads supporting (aligned to +/- strand):  ref base T (17/25);  new base A (2/2);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

CCGTCGGGTCTGCATTCAAAGAATGGTTGTTGTAGGAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGACATTGCGCATTAACCCCGGAACGCGTTCGCTGTCGCTGGGCTACAGCTTTTCCAAATGTG  >  NC_002947/2721253‑2721546
                                                                                                                                                   |                                                                                                                                                  
ccGTCGGGTCTGCATTCAAAGAATGGTTGTTGTAGGAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAAtc                                                                                                                                                   <  1:169429/149‑1 (MQ=255)
     gggTCTGCATTCAAAGAATGGTTGTTGTAGGAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAAt                                                                                                                                              <  8:44521/149‑1 (MQ=255)
        tCTGCATTCAAAGAATGGTTGTTGTAGGAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGATTGCCAAATATTGGCCAACTCAATcta                                                                                                                                           <  3:247763/149‑3 (MQ=38)
            cATTCAAAGAATGGTTGTTGTAGGAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTc                                                                                                                                        <  4:69806/148‑1 (MQ=255)
            cATTCAAAGAATGGTTGTTGTAGGAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGATTGCCAAATATTGGCCAACTCAATctaa                                                                                                                                          <  2:65083/146‑4 (MQ=38)
             aTTCAAAGAATGGTTGTTGTAGGAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCa                                                                                                                                       >  4:136959/1‑148 (MQ=255)
                 aaaGAATGGTTGTTGTAGGAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGa                                                                                                                                  <  3:311908/149‑1 (MQ=255)
                   aGAATGGTTGTTGTAGGAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGt                                                                                                                                <  7:139996/149‑1 (MQ=255)
                   aGAATGGTTGTTGTAGGAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGt                                                                                                                                <  5:138419/149‑1 (MQ=255)
                        ggTTGTTGTAGGAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCa                                                                                                                           >  4:382184/1‑149 (MQ=255)
                               gtAGGAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGaa                                                                                                                     <  6:415647/148‑1 (MQ=255)
                                tAGGAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATc                                                                                                                   >  4:26978/1‑149 (MQ=255)
                                  ggAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAAtag                                                                                                                                            >  1:265023/1‑120 (MQ=255)
                                  ggAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCg                                                                                                                                            <  2:265023/122‑1 (MQ=255)
                                  ggAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCt                                                                                                                  <  6:121081/148‑1 (MQ=255)
                                  ggAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCt                                                                                                                  >  7:380032/1‑148 (MQ=255)
                                   gAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTg                                                                                                                 >  5:178517/1‑148 (MQ=255)
                                     aaaTTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCGCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCaa                                                                                                              <  2:328877/149‑1 (MQ=255)
                                        ttCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATc                                                                                                            >  8:132589/1‑148 (MQ=255)
                                           cGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCaa                                                                                                         <  3:136959/148‑1 (MQ=255)
                                                  cTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGccc                                                                                                                            <  1:181558/122‑1 (MQ=255)
                                                  cTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGccc                                                                                                                            >  2:181558/1‑122 (MQ=255)
                                                   tGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAg                                                                                                 >  7:67048/1‑148 (MQ=255)
                                                     aTTTTCGCTTGCAGATAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATGCTTGCCGAGTCTTAGATTGAGTTGGCCATTATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGa                                                                                               <  5:411482/148‑1 (MQ=255)
                                                     aTTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAg                                                                                                 <  8:237735/146‑1 (MQ=255)
                                                          cGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGAt                                                                                          <  8:67048/148‑1 (MQ=255)
                                                             ttGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCg                                                                                      >  4:146754/1‑149 (MQ=255)
                                                                    aaGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGaa                                                                               <  5:216460/149‑1 (MQ=255)
                                                                           ggCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTAccc                                                                         <  1:305766/148‑1 (MQ=255)
                                                                            gCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGc                                                                       <  3:26978/149‑1 (MQ=255)
                                                                              gttgttACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTa                                                                     <  7:132589/149‑1 (MQ=255)
                                                                                     cAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGa                                                              <  8:380032/149‑1 (MQ=255)
                                                                                      aaCTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCAATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGa                                                              >  7:225300/1‑148 (MQ=255)
                                                                                         tttCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGACAtt                                                          >  4:161551/1‑149 (MQ=255)
                                                                                         tttCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGACAtt                                                          >  1:406643/1‑149 (MQ=255)
                                                                                          ttCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGcc                                                                                    >  7:330040/1‑122 (MQ=255)
                                                                                          ttCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGcc                                                                                    <  8:330040/122‑1 (MQ=255)
                                                                                           tCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGACATTg                                                         <  7:46413/148‑1 (MQ=255)
                                                                                             aTGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGACATTgcg                                                       <  3:382184/148‑1 (MQ=255)
                                                                                             aTGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGACATTgcg                                                       <  2:66093/148‑1 (MQ=255)
                                                                                                                       ttAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGTGATGTCGCCAGGAAGTACCCGCTAATGCTGACATTGCGCATTAACCCCGGAACGCGTtcgctgtc                            >  2:105479/1‑149 (MQ=255)
                                                                                                                          gATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGACATTGCGCATTAACCCCGGAACGCGTtcgctgtcgct                         >  5:132300/1‑149 (MQ=255)
                                                                                                                          gATTGAGATTGCCAAATATTGGCCAACTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGACATTGCGCATTAACCCCGGAACGCGTtcgctgtcgct                         >  2:218972/1‑149 (MQ=255)
                                                                                                                           aTTGAGATTGCCAAATATTGGCCAACTCAATCGGCTCAATGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGACATTGCGCATTAACCCCGGAACGCGTtcgctgtcgctg                        >  4:75567/1‑149 (MQ=255)
                                                                                                                               aGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGACATTGCGCATTAACCCCGGAACGCGTTCGCTGTCGCTGGGc                     <  6:178517/148‑1 (MQ=255)
                                                                                                                                      cAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGACATTGCGCATTAACCCCGGAACGCGTTCGCTGTCGCTGGGCTACAGCtt             >  1:7149/1‑149 (MQ=255)
                                                                                                                                                 aaTCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGACATTGCGCATTAACCCCGGAACGCGTTCGCTGTCGCTGGGCTACAGCTTTTCCAAAtgtg  <  3:161551/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCGTCGGGTCTGCATTCAAAGAATGGTTGTTGTAGGAAAATTCCGGGTTTCTGATTTTCGCTTGCAGAAAGCATCGGCGTTGTTACAACTTTCATGTTGAGCTCAATCCTGGCCGAGTCTTAGATTGAGTTGGCCAATATTTGGCAATCTCAATCGGCTCAAAGGAGTGCCCATATGAATCTGCAATCCAAGTGTTCAGGAGCGATGTCGCCAGGAAGTACCCGCTAATGCTGACATTGCGCATTAACCCCGGAACGCGTTCGCTGTCGCTGGGCTACAGCTTTTCCAAATGTG  >  NC_002947/2721253‑2721546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑