Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 213,220 G→C 7.8% V6242V (GTG→GTC)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947213,2200GC 7.8% 226.7 / 7.4 74V6242V (GTG→GTC) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (29/39);  new base C (4/2);  total (33/41)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

TGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGA  >  NC_002947/213075‑213362
                                                                                                                                                 |                                                                                                                                              
tGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGAcc                                                                                                                                             <  2:103083/149‑1 (MQ=255)
  ggCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGATGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACcga                                                                                                                                           <  3:380208/149‑1 (MQ=255)
    cACTGCCACCGTCACTGCCCCGGTCAATGTCTAGGTCGGTACCAACGACACTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTAGGCAAGATCGATCACCTGACGCAGGACAAGACGCCGGTCAGAACGTCGGTCACagacg                                                                                                                                         >  1:319065/1‑149 (MQ=17)
     aCTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACcgacga                                                                                                                                        <  7:108687/149‑1 (MQ=255)
         ccACCTTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGTCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACTAGACGCCGGTCAGCACGTCGGTCACCGACGTGc                                                                                                                                      <  2:319065/147‑1 (MQ=17)
         ccACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGc                                                                                                                                      <  8:111944/147‑1 (MQ=255)
          cACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCgg                                                                                                                                   <  5:406558/149‑1 (MQ=255)
           aCCGTCACTGCCCCGGACAATGTCTACGTCGGTAACAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGCGCAACTGACGCTGGACAAGACGCCGGTCAGCACCTCGGTGACCGCCGAGCCggg                                                                                                                                  >  6:215544/1‑149 (MQ=255)
            ccGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCggg                                                                                                                                  >  3:211072/1‑148 (MQ=255)
             cGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTa                                                                                                                                <  4:377769/149‑1 (MQ=255)
                 aCTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTAccc                                                                                                                             <  4:211072/148‑1 (MQ=255)
                  cTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTAccc                                                                                                                             >  4:37052/1‑147 (MQ=255)
                     ccccGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAgg                                                                                                                         >  1:98143/1‑148 (MQ=255)
                      cccGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGc                                                                                                                        >  7:129438/1‑148 (MQ=255)
                          gACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACg                                                                                                                    >  8:107560/1‑148 (MQ=255)
                          gACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACg                                                                                                                    >  7:135798/1‑148 (MQ=255)
                               tGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGc                                                                                                               >  7:232749/1‑148 (MQ=255)
                                   tACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAg                                                                                                                 <  2:121875/142‑1 (MQ=255)
                                   tACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAg                                                                                                                 >  1:121875/1‑142 (MQ=255)
                                     cGTCGGTACCAACGATCCTGTCGTGATGTCTTTCGCAACCGTTGTAGGCGCTGACGTTGGCTAGTTCGATCAAGTGAGGCTGGTCATTTCGCGGGTCAGCAGGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGTCGACttt                                                                                                         <  6:89280/148‑2 (MQ=17)
                                      gTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGt                                                                                                       <  3:37052/149‑1 (MQ=255)
                                      gTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGATACCCCAGGCAACGAAGGCGACTTGGt                                                                                                       <  7:222103/149‑1 (MQ=255)
                                           tACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAgg                                                                                                  >  4:332128/1‑149 (MQ=255)
                                            aCCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGt                                                                                                 >  4:187454/1‑149 (MQ=255)
                                             ccAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGt                                                                                                 <  8:135798/148‑1 (MQ=255)
                                               aaCGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAATGAAGGCGACTTGGTCAAGGTCAc                                                                                              <  6:411/149‑1 (MQ=255)
                                               aaCGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGaa                                                                                                                  <  4:300151/129‑1 (MQ=255)
                                               aaCGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGaa                                                                                                                  >  3:300151/1‑129 (MQ=255)
                                               aaCGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGATCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCAc                                                                                              >  4:223697/1‑149 (MQ=255)
                                                aCGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCAcc                                                                                             <  8:67133/149‑1 (MQ=255)
                                                    cccTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCa                                                                                         <  6:175988/149‑1 (MQ=255)
                                                              aTGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCa                                                                               <  1:392006/149‑1 (MQ=255)
                                                               tGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGc                                                                                                                        <  2:301228/107‑1 (MQ=255)
                                                               tGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGc                                                                                                                        >  1:301228/1‑107 (MQ=255)
                                                               tGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCa                                                                               <  7:107560/148‑1 (MQ=255)
                                                               tGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCa                                                                               <  8:232749/148‑1 (MQ=255)
                                                                 tCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGAc                                                                            >  1:377234/1‑149 (MQ=255)
                                                                     tCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCg                                                                        <  7:381727/149‑1 (MQ=255)
                                                                     tCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCg                                                                        <  1:92812/149‑1 (MQ=255)
                                                                         aaCCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGc                                                                                                               <  4:182503/106‑1 (MQ=255)
                                                                         aaCCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTgg                                                                    >  3:7799/1‑149 (MQ=255)
                                                                         aaCCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTgg                                                                    >  6:18467/1‑149 (MQ=255)
                                                                          aCCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAGTGTCACCATCACGGCCGACCAGACCTCGGTGGc                                                                   >  2:348483/1‑149 (MQ=255)
                                                                           ccGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGcc                                                                  >  5:281982/1‑149 (MQ=255)
                                                                             gTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCga                                                                <  4:303584/149‑1 (MQ=255)
                                                                                 aaGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAAc                                                            <  5:58513/149‑1 (MQ=255)
                                                                                   ggCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACgc                                                          >  5:244623/1‑148 (MQ=255)
                                                                                   ggCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGt                                                          >  2:143823/1‑149 (MQ=255)
                                                                                    gcgcGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTc                                                         <  5:18467/149‑1 (MQ=255)
                                                                                         gACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGTCGACCAGACCTCGGTGGCCGAGAACGTCAAGcc                                                    <  3:332128/149‑1 (MQ=255)
                                                                                         gACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGcc                                                    >  2:331765/1‑149 (MQ=255)
                                                                                            gTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGAc                                                 <  3:187454/149‑1 (MQ=255)
                                                                                                gCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGtt                                              >  4:107708/1‑148 (MQ=255)
                                                                                                gCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTc                                             <  5:68021/149‑1 (MQ=255)
                                                                                                  aaGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCAc                                           <  1:143823/149‑1 (MQ=255)
                                                                                                              cTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTCCACATCaa                               >  7:234189/1‑149 (MQ=255)
                                                                                                                   gCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCg                          <  3:85619/149‑1 (MQ=255)
                                                                                                                        aCAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTg                     <  2:377234/149‑1 (MQ=255)
                                                                                                                         cAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTgg                    <  2:98143/149‑1 (MQ=255)
                                                                                                                            gACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGc                                                                                                               <  7:366168/55‑1 (MQ=255)
                                                                                                                            gACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGc                                                                                                               >  8:366168/1‑55 (MQ=255)
                                                                                                                            gACGCCGGTCAGCACGTCGGTGACCGACCAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCt                  >  1:229473/1‑148 (MQ=255)
                                                                                                                             aCGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCa                >  5:13691/1‑149 (MQ=255)
                                                                                                                                 cGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGCCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGAc            <  6:244623/149‑1 (MQ=255)
                                                                                                                                      aGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTTGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGt       <  4:7799/149‑1 (MQ=255)
                                                                                                                                      aGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGt       >  2:67277/1‑149 (MQ=255)
                                                                                                                                       gctcGTCGGTCACCGACGTGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTc      <  5:64209/146‑1 (MQ=255)
                                                                                                                                       gctcGTCGGTCACCGACGAGCCGGGTA‑CCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCg     >  3:83802/4‑149 (MQ=255)
                                                                                                                                       gctcGTCGGTCACCGACGAGCCGGGTA‑CCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCg     >  3:89303/4‑149 (MQ=255)
                                                                                                                                       gCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTc      <  5:267581/149‑1 (MQ=255)
                                                                                                                                       gCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTc      >  6:267581/1‑149 (MQ=255)
                                                                                                                                         tcGTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCGAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCATGACCTGGTCGt    >  3:255530/2‑149 (MQ=255)
                                                                                                                                         aCGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGt    <  8:129438/149‑1 (MQ=255)
                                                                                                                                           gTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGa  <  1:67277/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGA  >  NC_002947/213075‑213362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑