Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,745,175	G→T	10.4%	intergenic (+18/+17)	PP_2399 → / ← PP_2400	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,745,175	0	G	T	10.4%	150.5 / 7.7	57	intergenic (+18/+17)	PP_2399/PP_2400	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (31/20);  new base T (3/3);  total (34/23)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.25e-01

GCGTATCGGTTGCTGGTGGAGCAGGGGACCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATG  >  NC_002947/2745029‑2745321                                                                                                                                                   |                                                                                                                                                   gccgaTCGGTTGCTGGTGGAGCAGGGGACCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGGGAAGGCATTGGGGGGCTGCgca                                                                                                                                                  <  2:126303/145‑1 (MQ=255)   gTATCGGTTGCTGGTGGAGCAGGGGACCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCgcagc                                                                                                                                                <  4:196099/149‑1 (MQ=255)        ggTTGCTGGTGGAGCAGGGGACCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGccc                                                                                                                                           <  7:169437/149‑1 (MQ=255)           tGCTGGTGGAGCAGGGGACCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCgcag                                                                                                                                                 >  1:361806/1‑140 (MQ=255)           tGCTGGTGGAGCAGGGGACCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCgcag                                                                                                                                                 <  2:361806/140‑1 (MQ=255)              tggtggAGCAGGGGACCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATCGGGGGGCTGCTGCGCAGccccccaat                                                                                                                                     <  2:188808/149‑5 (MQ=255)                gtggAGCAGGGGACCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTAGGCATTGCTGGAAGCATTTCCCGAGCAGCGTGTCATTGACGGAAAAGTGTGAAGGCATTGGGGGGATGCGCAGCAGCCCCCGATcc                                                                                                                                    >  7:166302/1‑148 (MQ=255)                           gACCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTAGGAACAGTGTGAAGGAATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGaa                                                                                                                        >  5:40186/1‑149 (MQ=255)                              cGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGcccc                                                                                                                                          >  7:113393/1‑128 (MQ=255)                              cGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGcccc                                                                                                                                          <  8:113393/128‑1 (MQ=255)                              cGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCg                                                                                                                     >  6:224167/1‑149 (MQ=255)                                    agagCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCTGCGCAGcccc                                                                                                                                          >  7:149522/1‑122 (MQ=255)                                    agagCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCTGCGCAGcccc                                                                                                                                          <  8:149522/122‑1 (MQ=255)                                         ggCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGc                                                                                                           >  5:133236/1‑148 (MQ=255)                                           cTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCATCATCTGCCGAACCGCATGCTCAGCCaa                                                                                                        >  1:406507/1‑149 (MQ=255)                                            tGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAAt                                                                                                       >  5:451/1‑149 (MQ=255)                                             gCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCTGCGCAGcccccc                                                                                                                                        >  1:409354/1‑114 (MQ=255)                                              cAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAAt                                                                                                       <  7:14234/147‑1 (MQ=255)                                                 gCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGGGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGc                                                                                                           <  7:169348/140‑1 (MQ=255)                                                 gCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGGGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGc                                                                                                           >  8:169348/1‑140 (MQ=255)                                                   aTTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATc                                                                                                   >  5:74716/1‑146 (MQ=255)                                                   aTTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGa                                                                                                 <  6:74716/148‑1 (MQ=255)                                                       ggCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCt                                                                                             >  3:280858/1‑148 (MQ=255)                                                         cGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCTGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAg                                                                                          <  8:239078/149‑1 (MQ=255)                                                                gtgCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAggcgcccg                                                                                   >  3:32745/1‑149 (MQ=255)                                                                  gcagcaAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAggcgcccggc                                                                                 >  3:96222/1‑149 (MQ=255)                                                                   cagcaAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAggcgcccggcg                                                                                >  4:8062/1‑149 (MQ=255)                                                                   cagcaAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAggcgcccggcg                                                                                >  1:164905/1‑149 (MQ=255)                                                                     gcaAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAggcgcccggcgcc                                                                              >  5:191913/1‑149 (MQ=255)                                                                      caAGGGGTGGCATTGCGGGAAGCATTTCGCGAGCAGGGGGTCATTGTCGGAACAGTGGGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAggcgcccggcgccc                                                                             <  7:235302/149‑1 (MQ=255)                                                                        aGGGTTGGAATTGCTGTAAGCATTTCGCGAGCTGGGTGTCATTGTCGGAACTGTGTTAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCtt                                                                           <  4:32745/149‑1 (MQ=255)                                                                          ggTTGGGTTTGGTGGAAGCATTTCGGTAGCAGGGGGGCTTTGTCGGCTCTGTGTGATGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCGATTGATGGAGGCTAAGGCGCCCGGCGCCCTTGa                                                                         <  8:166302/149‑1 (MQ=255)                                                                           gTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAggcgcccggcgc                                                                               <  3:113164/142‑1 (MQ=255)                                                                           gTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAggcgcccggcgc                                                                               >  4:113164/1‑142 (MQ=255)                                                                               gCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGagcag                                                                     >  2:127824/1‑148 (MQ=255)                                                                                cATTGCTGGAAGCATTTCGCGAGCAGGGTGCCATTGGCGGAACAGGGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAgcagca                                                                   <  7:184491/149‑1 (MQ=255)                                                                                 atgtcgggAACCATTTCGCGAGCCGGGGGTCAGTGTCGTAACAGTGTGAAGGCATGGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGcagcag                                                                  <  8:230234/143‑1 (MQ=255)                                                                                 aTTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTACGGCGCCCGGCGCCCTTGAGcagcag                                                                  >  8:349307/1‑149 (MQ=255)                                                                                 aTTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGcagcag                                                                  >  5:99588/1‑149 (MQ=255)                                                                                    gCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTgg                                                               >  6:336099/1‑149 (MQ=255)                                                                                     cTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTgg                                                               <  3:333488/148‑1 (MQ=255)                                                                                     cTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTgg                                                               <  1:127824/148‑1 (MQ=255)                                                                                             aTTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGAAACGc                                                      >  4:246324/1‑149 (MQ=255)                                                                                              tttCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGc                                                      >  2:173154/1‑148 (MQ=255)                                                                                                  gcgAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGccac                                                   >  3:351345/1‑147 (MQ=255)                                                                                                     cgccgcgtgcCATTGCCCGAACCGTCTGAACGCATGGGGCGGCCGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGt                                              <  4:280858/143‑1 (MQ=255)                                                                                                              tCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCg                                     >  8:116384/1‑149 (MQ=255)                                                                                                               cATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCgg                                    >  8:119458/1‑149 (MQ=255)                                                                                                                 ttGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGc                                   >  3:152806/1‑148 (MQ=255)                                                                                                                    tCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACt                               >  6:405249/1‑149 (MQ=255)                                                                                                                     cGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACtt                              <  1:173154/149‑1 (MQ=255)                                                                                                                         aCAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTaa                          <  4:96222/149‑1 (MQ=255)                                                                                                                             tgtgAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAAtt                        <  2:274752/147‑1 (MQ=255)                                                                                                                             tgtgAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAAtt                        >  1:274752/1‑147 (MQ=255)                                                                                                                                  aGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGaa                  <  5:336099/148‑1 (MQ=255)                                                                                                                                      aTTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCt             >  4:263443/1‑149 (MQ=255)                                                                                                                                        tGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTgg           >  6:164125/1‑149 (MQ=255)                                                                                                                                        ggggctgCTGCGCAGC‑‑CCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTc         >  6:251402/7‑149 (MQ=255)                                                                                                                                         cgggggCTGCTGCGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGt          >  3:307252/2‑149 (MQ=255)                                                                                                                                          gggggCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGt          <  7:349307/148‑1 (MQ=255)                                                                                                                                           ggggCTGCTGCGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCg        <  4:307252/149‑1 (MQ=255)                                                                                                                                                 gcgcAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAg                 <  7:119009/134‑1 (MQ=255)                                                                                                                                                 gcgcAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAg                 >  8:119009/1‑134 (MQ=255)                                                                                                                                                 gcgcAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATg  <  3:8062/149‑1 (MQ=255)                                                                                                                                                  cgcAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATg  <  7:116384/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GCGTATCGGTTGCTGGTGGAGCAGGGGACCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCATTGGGGGGCTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATG  >  NC_002947/2745029‑2745321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑