Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,774,810 T→C 9.3% P234P (CCA→CCG) ‡ PP_5521 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,774,8100TC 9.3% 144.0 / 5.1 55P234P (CCA→CCG) ‡PP_5521hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (22/27);  new base C (3/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

GTCCAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCAAC  >  NC_002947/2774663‑2774957
                                                                                                                                                   |                                                                                                                                                   
gtCCAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGGCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCtgt                                                                                                                                                     <  8:385532/148‑1 (MQ=255)
      aaCAAGATATCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACAGCACCATGCTCCATGCTCAGCCCCAGGCGGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTTCTGTGgcag                                                                                                                                                >  2:219268/1‑147 (MQ=255)
       aCAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGCCAcagcag                                                                                                                                             >  4:250188/1‑149 (MQ=255)
         aaGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCTGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGc                                                                                                                                           <  1:42486/149‑1 (MQ=255)
         aaGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCTGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGc                                                                                                                                           >  2:42486/1‑149 (MQ=255)
           gATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGc                                                                                                                                           >  5:226973/1‑147 (MQ=255)
           gATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGCCACAGCAGGcac                                                                                                                                         <  3:250188/149‑3 (MQ=255)
             tGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGt                                                                                                                                       <  7:283898/149‑1 (MQ=255)
              gTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTc                                                                                                                                      >  7:130734/1‑149 (MQ=255)
               tCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCg                                                                                                                                     <  6:354264/149‑1 (MQ=255)
                           cTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTg                                                                                                                          <  5:99792/148‑1 (MQ=255)
                            tCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGCCACAGCAGGcac                                                                                                                                         >  6:394435/1‑130 (MQ=255)
                            tCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGCCACAGCAGGcac                                                                                                                                         <  5:394435/132‑3 (MQ=255)
                              aCCCACGACGATCAGCCGGCCTTCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCGCGGCGAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCa                                                                                                                      <  1:219268/149‑1 (MQ=255)
                              aCCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGc                                                                                                                        <  7:201476/147‑1 (MQ=255)
                              aCCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCa                                                                                                                      <  8:130734/149‑1 (MQ=255)
                                ccACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTc                                                                                                                                      >  6:163179/1‑131 (MQ=255)
                                ccACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTc                                                                                                                                      <  5:163179/131‑1 (MQ=255)
                                ccACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAg                                                                                                                     >  4:25857/1‑148 (MQ=255)
                                 cACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCTGAGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTa                                                                                                                   >  3:28763/1‑149 (MQ=255)
                                 cACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTa                                                                                                                   >  4:143671/1‑149 (MQ=255)
                                  acgacgATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTAc                                                                                                                  >  4:105029/1‑149 (MQ=255)
                                  acgacgATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTCCCTGCTGAGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTAc                                                                                                                  >  8:246915/1‑149 (MQ=255)
                                        aTCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGAt                                                                                                            <  7:26565/149‑1 (MQ=255)
                                                          gACACCGCACCCTGCTCCATGCTCACCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGTCCAGTACGAAGATACCTTCGGCGCTCACcag                                                                                          <  4:37118/149‑1 (MQ=255)
                                                          gACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACcag                                                                                          <  5:220143/149‑1 (MQ=255)
                                                            cacCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACcagca                                                                                        <  8:146866/149‑1 (MQ=255)
                                                            cacCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACcagca                                                                                        <  7:54411/149‑1 (MQ=255)
                                                             acCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACcagcag                                                                                       >  1:369364/1‑149 (MQ=255)
                                                                  aCCATGCTCCATGCCCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTc                                                                                  >  2:16364/1‑149 (MQ=255)
                                                                     atgctccatgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCt                                                                               <  2:369364/149‑1 (MQ=255)
                                                                                tcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCggg                                                                     >  1:357508/1‑148 (MQ=255)
                                                                                tcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCACACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCggg                                                                     >  8:175821/1‑148 (MQ=255)
                                                                                   gCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAg                                                                 <  3:105029/149‑1 (MQ=255)
                                                                                    ccccAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAgg                                                                <  3:143671/149‑1 (MQ=255)
                                                                                     cccAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAggg                                                               <  3:25857/149‑1 (MQ=255)
                                                                                       cAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGa                                                             >  8:336273/1‑149 (MQ=255)
                                                                                        aGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGAt                                                            >  4:307269/1‑149 (MQ=255)
                                                                                              aTGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAgcgc                                                      <  5:208940/149‑1 (MQ=255)
                                                                                                gtcgtcCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCg                                                                                                                                     >  7:169036/1‑68 (MQ=255)
                                                                                                gtcgtcCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCg                                                                                                                                     <  8:169036/68‑1 (MQ=255)
                                                                                                gtcgtcCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGcgg                                                    >  6:56364/1‑149 (MQ=255)
                                                                                                    tcCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGcggtcgg                                                >  4:351750/1‑149 (MQ=255)
                                                                                                      cGGGTCGGTGGCCTCGGCCATCTCTTCGTGCTCGCGTGCCTGCTGTGGCAGCAGGAGAGTCGGACCTTGACACGCCAGTACGAAGATACCTTGGGCGCTCACCTGCAGTTGTCGCTGTCCATCGTGGAAGGGGAGCTGCGcgggcggt                                               <  4:28763/148‑1 (MQ=255)
                                                                                                       gggTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGgtgt                                             >  7:242351/1‑149 (MQ=255)
                                                                                                        ggTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGtgtg                                            <  1:16364/149‑1 (MQ=255)
                                                                                                                cccTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAg                                    <  6:226973/149‑1 (MQ=255)
                                                                                                                 ccTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAgg                                   <  7:336273/149‑1 (MQ=255)
                                                                                                                 ccTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAgg                                   >  3:119948/1‑149 (MQ=255)
                                                                                                                      gCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGaa                              <  2:357508/149‑1 (MQ=255)
                                                                                                                           ctctTCGAGCACGCGTGCCGGCTGTGGCAGCAGGCGAGTCGCACCTTGAGGGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCt                         <  3:299768/149‑1 (MQ=255)
                                                                                                                              ttCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTc                        <  3:307269/147‑1 (MQ=255)
                                                                                                                                   gCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACggg                 >  3:65925/1‑149 (MQ=255)
                                                                                                                                         tcgCCTGCTGCCACAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCt           >  3:364832/3‑149 (MQ=255)
                                                                                                                                           gCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTgg         >  2:223596/1‑149 (MQ=255)
                                                                                                                                            ccTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTGACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTggc        <  7:246915/149‑1 (MQ=255)
                                                                                                                                                  gtgGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTaa              >  1:407099/1‑137 (MQ=255)
                                                                                                                                                  gtgGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTaa              <  2:407099/137‑1 (MQ=255)
                                                                                                                                                  gtgGCAGCAGGCGAGTCACACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCAAc  <  7:175821/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTCCAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCAGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCAAC  >  NC_002947/2774663‑2774957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑