Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,776,839 G→A 11.9% V396I (GTT→ATT) ‡ sotB → sugar efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,776,8390GA11.9% 172.2 / 11.9 67V396I (GTT→ATT) ‡sotBsugar efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (29/30);  new base A (3/5);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01

ATGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCC  >  NC_002947/2776693‑2776984
                                                                                                                                                  |                                                                                                                                                 
aTGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGtt                                                                                                                                                 <  4:271407/149‑1 (MQ=255)
     gCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAg                                                                                                                                            >  5:229322/1‑149 (MQ=255)
           gcgcTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCAACGGCCAGTAcca                                                                                                                                       <  1:243149/148‑2 (MQ=255)
           gcgcTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCAACGGCCAGTAcca                                                                                                                                       <  8:352934/148‑2 (MQ=255)
             gcTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGgc                                                                                                                                    <  6:229322/149‑1 (MQ=255)
                       ggTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGa                                                                                                                          <  7:164146/149‑1 (MQ=255)
                        gTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAg                                                                                                                         >  3:63566/1‑149 (MQ=255)
                             tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCtt                                                                                                                                >  6:18236/1‑137 (MQ=255)
                             tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCtt                                                                                                                                <  5:18236/137‑1 (MQ=255)
                             tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGtt                                                                                                                    >  1:128701/1‑149 (MQ=255)
                             tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGtt                                                                                                                    >  6:320828/1‑149 (MQ=255)
                              acaacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGTCGGATATCGGCCTGGTGGGCGGCAGCGTGGAAGTGGTCGGCCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGtt                                                                                                                    >  1:214970/1‑148 (MQ=255)
                              acaacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGtt                                                                                                                    >  6:191864/1‑148 (MQ=255)
                              acaacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGtt                                                                                                                    >  7:97751/1‑148 (MQ=255)
                               caacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGtt                                                                                                                    <  5:320828/147‑1 (MQ=255)
                               caacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGt                                                                                                                     <  5:185374/146‑1 (MQ=255)
                               caacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGa                                                                                                                  >  2:188492/1‑149 (MQ=255)
                               caacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGa                                                                                                                  >  7:67710/1‑149 (MQ=255)
                               caacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCAAGATGGATGTGGAGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCATGCGCTTTAGCGAGGCGTTg                                                                                                                   >  6:287009/1‑148 (MQ=255)
                                    tCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCAACGGCCAGTAc                                                                                                                                         <  2:79952/121‑1 (MQ=255)
                                    tCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCAACGGCCAGTAc                                                                                                                                         >  1:79952/1‑121 (MQ=255)
                                     cGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGtt                                                                                                            >  1:68280/1‑149 (MQ=255)
                                      ggCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCGGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGtt                                                                                                            <  7:260586/148‑1 (MQ=255)
                                       gCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCTGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGa                                                                                                          <  5:287009/149‑1 (MQ=255)
                                        cATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAg                                                                                                         >  8:131500/1‑149 (MQ=255)
                                             gCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGa                                                                                                     <  6:368041/148‑1 (MQ=255)
                                             gCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAg                                                                                                    <  6:384879/149‑1 (MQ=255)
                                              cGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAgg                                                                                                   <  2:188357/149‑1 (MQ=255)
                                                    tgCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTg                                                                                                           >  4:397009/1‑135 (MQ=255)
                                                    tgCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTg                                                                                                           <  3:397009/135‑1 (MQ=255)
                                                                    aTCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGt                                                                              <  8:67710/148‑1 (MQ=255)
                                                                        tCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGt                                                                         <  7:120824/149‑1 (MQ=255)
                                                                         cagcagCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCa                                                                            >  4:228/1‑145 (MQ=255)
                                                                         cagcagCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCa                                                                            <  3:228/145‑1 (MQ=255)
                                                                          agcagcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAg                                                                          >  6:142875/1‑146 (MQ=255)
                                                                          agcagcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTa                                                                        <  5:84258/148‑1 (MQ=255)
                                                                           gcagcCAGATGGATGTGGCGGATATCGGCGTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTa                                                                        >  5:238301/1‑147 (MQ=255)
                                                                             agcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAgg                                                                    <  5:142875/149‑1 (MQ=255)
                                                                                    tggatgTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGc                                                             >  4:4210/1‑149 (MQ=255)
                                                                                     ggatgTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGc                                                             <  2:128701/148‑1 (MQ=255)
                                                                                        tgtgGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAAt                                                         <  2:68280/149‑1 (MQ=255)
                                                                                          tgGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATa                                                        <  1:188492/148‑1 (MQ=255)
                                                                                            gCGGATATCGGCCTGGTGGGCGGCAGCGTGGTAGTGGTCGGGCTGGTACTGGCAGTTGCCAGTAGCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCGGTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTg                                                     <  2:214970/149‑1 (MQ=255)
                                                                                            gCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACt                                                      <  3:4210/148‑1 (MQ=255)
                                                                                             cGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGc                                                    <  5:191864/149‑1 (MQ=255)
                                                                                                      gCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGc                                           >  3:265475/1‑149 (MQ=255)
                                                                                                          ggtggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGa                                        >  1:242909/1‑148 (MQ=255)
                                                                                                           gtggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGata                                      >  8:56030/1‑149 (MQ=255)
                                                                                                            tggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGatat                                     <  4:63566/149‑1 (MQ=255)
                                                                                                              ggcggcAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCTAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATc                                    >  2:369232/1‑148 (MQ=255)
                                                                                                                  ggaGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCGATTACTGCAGCCCATGCCGATATgaaca                                >  7:53473/3‑148 (MQ=255)
                                                                                                                  gcAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATcaaca                                >  7:53475/1‑148 (MQ=255)
                                                                                                                     gggtagCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCGTTAGCGAGGCGTTGACACGTTGAGCCGAGGCCGCGCAGGCCCCGTGTCGGGCAAGTAGAGGCTCAGGGCAAAACTGCAGCCCAGTCCGATATCAACAAAAc                            >  2:127094/6‑149 (MQ=255)
                                                                                                                      cGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAAcc                           <  7:138469/149‑1 (MQ=255)
                                                                                                                             gTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGcc                     >  8:14606/1‑148 (MQ=255)
                                                                                                                                     gCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTgg            >  1:394151/1‑149 (MQ=255)
                                                                                                                                     ccgggTACTGGCAACGGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGc                                                    <  7:97205/106‑1 (MQ=255)
                                                                                                                                     ccgggTACTGGCAACGGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGc                                                    >  8:97205/4‑109 (MQ=255)
                                                                                                                                      cgggTACTGGCAACGGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGcccc                                                                                      >  6:130966/3‑74 (MQ=37)
                                                                                                                                      cgggTACTGGCAACGGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGcccc                                                                                      <  5:130966/72‑1 (MQ=37)
                                                                                                                                       tGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTgg            <  8:386314/147‑1 (MQ=255)
                                                                                                                                       tGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTgg            >  7:386314/1‑147 (MQ=255)
                                                                                                                                         gTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGtt          <  6:238301/147‑1 (MQ=255)
                                                                                                                                          tACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGaa       <  7:131500/149‑1 (MQ=255)
                                                                                                                                             tGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAaccc    >  1:393793/1‑147 (MQ=255)
                                                                                                                                               gCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACAccc  <  2:242909/149‑1 (MQ=255)
                                                                                                                                               gCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACAccc  <  7:14606/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCC  >  NC_002947/2776693‑2776984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑