Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,799,302	G→C	8.1%	A330G (GCT→GGT) ‡	ansB ←	glutaminase‑asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,799,302	0	G	C	8.1%	193.4 / 6.1	62	A330G (GCT→GGT) ‡	ansB	glutaminase‑asparaginase
Reads supporting (aligned to +/- strand):  ref base G (28/29);  new base C (2/3);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

GGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTGCGCAGTTCTTGCAGCGCCGGGACTACCCGCGACGATACCGAACCGT  >  NC_002947/2799162‑2799449                                                                                                                                                |                                                                                                                                                    ggCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGAcc                                                                                                                                             <  3:347424/149‑1 (MQ=255) ggCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGAcc                                                                                                                                             <  2:307609/149‑1 (MQ=255)  gCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGGCTCACGAcct                                                                                                                                            <  8:100153/149‑2 (MQ=255)   cccTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGAcc                                                                                                                                             >  3:350471/1‑147 (MQ=255)   cccTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGAc                                                                                                                                              >  7:165619/1‑146 (MQ=255)   cccTCAGGGCCGGTGCGCCGGCGCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGAc                                                                                                                                              <  8:165619/146‑1 (MQ=255)      tCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGCGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTc                                                                                                                                        >  1:127270/1‑149 (MQ=255)      tCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTCCCATCGCCAGGATCCGCGCTTGTTGCGGGTTCCTGTCGTGAGCCCCGCCCCGGTc                                                                                                                                        >  4:310220/1‑149 (MQ=255)      tCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTc                                                                                                                                        >  1:218856/1‑149 (MQ=255)        agagCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGGCTCACGAcctgaacc                                                                                                                                       <  3:184753/148‑7 (MQ=255)        agagCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGGCTCACGAcctgaacc                                                                                                                                       <  1:147810/148‑7 (MQ=255)        agagCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTc                                                                                                                                        <  6:45834/147‑1 (MQ=255)          agCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTc                                                                                                                                    <  4:251285/149‑1 (MQ=255)               gcgcACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATACTCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCtt                                                                                                                                  <  1:126033/149‑1 (MQ=255)               gcgcACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTc                                                                                                                               <  8:6304/149‑1 (MQ=255)                    ccGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCg                                                                                                                          >  6:393763/1‑149 (MQ=255)                          cTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGtt                                                                                                                    <  3:334203/149‑1 (MQ=255)                                    aGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACg                                                                                                          <  2:287445/149‑1 (MQ=255)                                      cACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCa                                                                                                        <  2:59035/149‑1 (MQ=255)                                         tCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTa                                                                                                     >  4:274304/1‑149 (MQ=255)                                         tCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTa                                                                                                     >  1:65935/1‑149 (MQ=255)                                         tCAGTACTCCCAGAAGATCCGCTGCAGCTCTTAGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCAACGCCAGGATACTCCCGTATTGCGGGATCACGACGTGAGCCACGCCCCAGTCGTTCTTGTAATCCGGATCTACGCCAATACGCAGTa                                                                                                     >  5:13162/1‑149 (MQ=255)                                           aGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTTAGCCACGACCCAGTCGTTCTTGTCATACGGCTGTTCGGCATTACGCa                                                                                                        <  6:173918/144‑1 (MQ=255)                                           aGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTAc                                                                                                    >  2:251122/1‑148 (MQ=255)                                           aGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACg                                                                                                   <  2:218856/149‑1 (MQ=255)                                           aGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTAATCCGGCTGTTCGGCATTACGCa                                                                                                        >  5:173918/1‑144 (MQ=255)                                               cTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAac                                                                                               >  6:352143/1‑149 (MQ=255)                                                   cAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCtt                                                                                                                                  <  3:225884/110‑1 (MQ=255)                                                   cAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCtt                                                                                                                                  >  4:225884/1‑110 (MQ=255)                                                      aagaTCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGACACGACCCAGTCGTTATTGTCGTCCGGCTGTTCGGCATTACGCAGTACGAAACCAcccgg                                                                                        >  8:149133/1‑147 (MQ=255)                                                              cTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACAt                                                                                <  3:171228/149‑1 (MQ=255)                                                                  aGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGcga                                                                            <  5:86852/149‑1 (MQ=255)                                                                         tGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCg                                                                      >  3:176315/1‑148 (MQ=255)                                                                           cTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGga                                                                    <  5:352143/148‑1 (MQ=255)                                                                           cTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGACATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGgat                                                                   >  4:202949/1‑149 (MQ=255)                                                                              tCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGgatgat                                                                <  4:125905/149‑1 (MQ=255)                                                                                cTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATAAGGCACTACGAACCCACCCGGGGTGACATGCGCCGACCGGATGctct                                                              >  4:98628/1‑149 (MQ=255)                                                                                   ggTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGc                                                                                                                        <  5:176665/88‑1 (MQ=255)                                                                                   ggTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGc                                                                                                                        >  6:176665/1‑88 (MQ=255)                                                                                     gcTTGGGCA‑‑‑TCGCTACCATCGCCAGTATCAGAGCTTTTTGAGGTTTCAGGTCGTGAGCCACGACCCAGTAGGTCTTGTAATCCTGCTTTTCGGCATTACTAAGTACGAAACCACACTTTTTGACATGCGACGAGCTGATGATCTGAACg                                                         <  6:77500/148‑1 (MQ=255)                                                                                             a‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCtt                                                 <  4:176936/149‑1 (MQ=255)                                                                                             a‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCtt                                                 <  2:127270/149‑1 (MQ=255)                                                                                                  cGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTgc                                               >  2:153853/1‑149 (MQ=255)                                                                                                   gCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTgc                                               <  4:350471/148‑1 (MQ=255)                                                                                                       ccATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATc                                                               >  8:54455/1‑128 (MQ=255)                                                                                                       ccATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATc                                                               <  7:54455/128‑1 (MQ=255)                                                                                                       ccATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTGCGCa                                            <  6:161755/147‑1 (MQ=255)                                                                                                        cATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTGCGCAGtt                                         <  2:65935/149‑1 (MQ=255)                                                                                                         aTCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTGCGCAGTTc                                        >  5:370269/1‑149 (MQ=255)                                                                                                                   tCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTGCGCAGTTCTTGCAGCGcc                              <  3:310220/149‑1 (MQ=255)                                                                                                                     cgcgCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCgg                                                                     <  8:82996/108‑1 (MQ=255)                                                                                                                     cgcgCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCgg                                                                     >  7:82996/1‑108 (MQ=255)                                                                                                                      gcgcTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTGCGCAGTTCTTGCAGCGCCgg                            >  5:34645/1‑148 (MQ=255)                                                                                                                      gcgcTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTGCGCAGTTCTTGCAGCGCCgg                            >  3:216295/1‑148 (MQ=255)                                                                                                                                     tCAGGTCGTGAGCCACGACCCAGTCGTTATTGTCATCCGGCTGTTCGGCATTACTCAGTACGACAACACCCTGGTTGACATGCGACGCGCGGATGATCTGAACGCCGTTCTTGGGCAGTTCTTGCAGCGCCGGGACAAGCCGcgccga             >  4:10237/1‑148 (MQ=255)                                                                                                                                      tgggTCGTGGCTCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTGCGCAGTTCTTGCAGCGCCGGGACTACCCGCGACGATa           >  1:305971/3‑149 (MQ=255)                                                                                                                                       aGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTGCGCAGTTCTTGCAGCGCCGGGACTACCCGCGACGATAc          >  1:374120/1‑149 (MQ=255)                                                                                                                                       aGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTGCGCAGTTCTTGCAGCGCCGGGACTACCCGCGACGATAc          <  5:393763/149‑1 (MQ=255)                                                                                                                                        ggTCGTGGCTCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGGATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTGCGCAGTTCTTGCAGCGCCGGGACTACCCGCGACGATAcc         >  1:286200/1‑149 (MQ=255)                                                                                                                                            gTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTGCGCAGTTCTTGCAGCGCCgg                            <  6:280702/126‑1 (MQ=255)                                                                                                                                            gTGAGCCACGACCCAGTCGTTCTTGTCATACGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTGCGCAGTTCTTGCAGCGCCgg                            >  5:280702/1‑126 (MQ=255)                                                                                                                                               aGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACACTGGTTGACATGCGAAGAGCGGATGATCTGCACGCCGTTCTTGCGCAGTTCTTGCAGCGCCGGGACTACCCGCGACCATACCGAACCGt  >  5:38445/1‑149 (MQ=255)                                                                                                                                                gCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGtt                                                                                     >  8:144837/1‑65 (MQ=255)                                                                                                                                                gCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGtt                                                                                     <  7:144837/65‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                    GGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCA‑‑‑TCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGTTCGGCATTACGCAGTACGAAACCACCCTGGTTGACATGCGACGAGCGGATGATCTGCACGCCGTTCTTGCGCAGTTCTTGCAGCGCCGGGACTACCCGCGACGATACCGAACCGT  >  NC_002947/2799162‑2799449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑