Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,801,973 C→A 5.6% R119R (CGC→CGA)  rbsA‑I → ribose ABC transporter ‑ ATP‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,801,9730CA 5.6% 227.1 / 2.1 70R119R (CGC→CGA) rbsA‑Iribose ABC transporter ‑ ATP‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (33/33);  new base A (2/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

CATGACCTACCGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGCCCGCAACCTGATC  >  NC_002947/2801829‑2802120
                                                                                                                                                |                                                                                                                                                   
caTGACCTACCGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCg                                                                                                                                                 <  6:269693/149‑1 (MQ=255)
 aTGACCTACCGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAATCTGCCCAGCCGCTTCgg                                                                                                                                                >  6:418457/1‑149 (MQ=255)
 aTGACCTACCGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCgg                                                                                                                                                <  5:313173/149‑1 (MQ=255)
 aTGACCTACCGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCgg                                                                                                                                                >  4:356087/1‑149 (MQ=255)
  tGACCTACCGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAATCTGCCCAGCCGCTTCGGc                                                                                                                                               <  5:418457/149‑1 (MQ=255)
   gACCTACCGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCt                                                                                                                                              >  4:163811/1‑149 (MQ=255)
    aCCTACCGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCt                                                                                                                                              <  2:45927/148‑1 (MQ=255)
       tACCGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGAAGCGGCTggg                                                                                                                                           >  8:73145/1‑147 (MQ=255)
         ccGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGAAGCGGCTgggcag                                                                                                                                        <  7:73145/149‑5 (MQ=255)
         ccGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGAAGCGGCTgggca                                                                                                                                         <  6:266800/148‑4 (MQ=255)
          ggcgGCCAGGCCTAGGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCa                                                                                                                                        <  5:92845/147‑1 (MQ=255)
           gcgGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGc                                                                                                                                      >  5:176263/1‑149 (MQ=255)
              gCCAGGCCTACGCGCCCGTCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCcac                                                                                                                                   <  3:259381/149‑1 (MQ=255)
                   gCCTACGTGCGGGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGGTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACGTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGc                                                                                                                               <  7:126769/148‑1 (MQ=255)
                   gCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCg                                                                                                                              >  5:109447/1‑149 (MQ=255)
                   gCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCg                                                                                                                              <  6:176263/149‑1 (MQ=255)
                    ccTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCca                                                                                                                                    <  5:152560/142‑1 (MQ=255)
                    ccTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCca                                                                                                                                    >  6:152560/1‑142 (MQ=255)
                           gcCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCcac                                                                                                                                   <  2:70028/136‑1 (MQ=255)
                           gcCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCcac                                                                                                                                   >  1:70028/1‑136 (MQ=255)
                            cccGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCa                                                                                                                     <  2:186626/149‑1 (MQ=255)
                             ccGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAg                                                                                                                    >  1:1541/1‑149 (MQ=255)
                             ccGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTCGATCAGCCACAAGCGTTTGCGCCAg                                                                                                                    >  4:218887/1‑149 (MQ=255)
                                 cAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTgg                                                                                                                <  5:419464/149‑1 (MQ=255)
                                  aGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGc                                                                                                               <  8:100209/149‑1 (MQ=255)
                                    ccGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGCCGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCaa                                                                                                             >  8:110741/1‑149 (MQ=255)
                                       cggcgAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACgg                                                                                                          >  3:316646/1‑149 (MQ=255)
                                                 gAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGc                                                                                                >  8:246502/1‑149 (MQ=255)
                                                   aCGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCtc                                                                                              <  7:9962/149‑1 (MQ=255)
                                                                 gcATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGAc                                                                                >  2:195187/1‑149 (MQ=255)
                                                                    tGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGc                                                                              >  4:120280/1‑148 (MQ=255)
                                                                     ggTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCa                                                                            >  3:122042/1‑149 (MQ=255)
                                                                       tCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCAtt                                                                          <  6:415560/149‑1 (MQ=255)
                                                                        cATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCAtt                                                                          >  1:245162/1‑148 (MQ=255)
                                                                          tGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGAc                                                                       <  2:245162/149‑1 (MQ=255)
                                                                            cAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGAccc                                                                     >  1:102874/1‑149 (MQ=255)
                                                                             aGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCg                                                                    <  8:117557/149‑1 (MQ=255)
                                                                                       ccTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGCTCGGGCTCGACGCCATTGACCCGGACACCCCTg                                                          >  5:109282/1‑149 (MQ=255)
                                                                                           ctgcCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCg                                                       <  4:122042/148‑1 (MQ=255)
                                                                                              ccGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCg                                                    >  8:64054/1‑148 (MQ=255)
                                                                                                   gCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTgg                                              <  6:109447/149‑1 (MQ=255)
                                                                                                    cTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCCGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTggg                                             <  4:316646/149‑1 (MQ=255)
                                                                                                     tGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTggg                                             >  4:219658/1‑148 (MQ=255)
                                                                                                     tGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTggg                                             >  2:385113/1‑148 (MQ=255)
                                                                                                     tGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTgg                                              >  8:338815/1‑147 (MQ=255)
                                                                                                     tGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTgg                                              <  7:338815/147‑1 (MQ=255)
                                                                                                       aCCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCAt                                          >  6:25117/1‑149 (MQ=255)
                                                                                                         cGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCg                                        <  3:163811/149‑1 (MQ=255)
                                                                                                               ggAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAAc                                                 >  4:160833/1‑134 (MQ=255)
                                                                                                               ggAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAAc                                                 <  3:160833/134‑1 (MQ=255)
                                                                                                                gAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCAGTGACCCGGACACACCAGTCGCAGAACTGGGCATCGGACATCa                                 >  4:319204/1‑149 (MQ=255)
                                                                                                                gAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACAGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCAt                                   >  3:217214/1‑147 (MQ=255)
                                                                                                                 aaaaCCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAg                                <  3:218887/149‑1 (MQ=255)
                                                                                                                           tctcGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTc                       <  7:246502/148‑1 (MQ=255)
                                                                                                                            ctcGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCg                      <  3:120280/148‑1 (MQ=255)
                                                                                                                            ctcGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCAGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCg                      <  6:119713/148‑1 (MQ=255)
                                                                                                                               gACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACCCCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGAt                  >  2:340884/1‑149 (MQ=255)
                                                                                                                               gACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCga                     <  3:219658/146‑1 (MQ=255)
                                                                                                                               gACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCga                     <  1:195187/146‑1 (MQ=255)
                                                                                                                               gACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTACCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCCTCGGCCATCAGCAAATGGGCGAGAt                  >  3:54347/1‑149 (MQ=255)
                                                                                                                                     tgatccAGCCGAAGCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACCCCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGCCCg            >  6:65560/5‑149 (MQ=255)
                                                                                                                                     cTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGac                                                                 >  8:67862/1‑96 (MQ=255)
                                                                                                                                     cTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGac                                                                 <  7:67862/96‑1 (MQ=255)
                                                                                                                                     cTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGCCCg            >  3:272843/1‑149 (MQ=255)
                                                                                                                                      tGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCAATGGCTCGGGTCGGGCTGGGCGCCATTGACCCGGCCACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATTGGGGAGATTGCCCg            >  1:114979/1‑148 (MQ=255)
                                                                                                                                         ccAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGCCCGCAAc        <  7:64054/149‑1 (MQ=255)
                                                                                                                                          cAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATc                                  <  3:275069/122‑1 (MQ=255)
                                                                                                                                          cAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATc                                  >  4:275069/1‑122 (MQ=255)
                                                                                                                                              cGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGCCCGCAACCTGAt   >  1:227333/1‑149 (MQ=255)
                                                                                                                                               gCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGCCCGCAACCTGATc  <  7:110741/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CATGACCTACCGCGGCCAGGCCTACGCGCCCGGCAGCCGCGGCGAGGCCGAACGCCTTGGGGTGCGCATGGTCATGCAGGAGCTGAACCTGCTGCCGACGCTGACCGTGGCGGAAAACCTGTTTCTCGACAACCTGCCCAGCCGCTTCGGCTGGATCAGCCACAAGCGTTTGCGCCAGCTGGCAACGGCTGCCATGGCTCGGGTCGGGCTCGACGCCATTGACCCGGACACCCCTGTCGGCGAACTGGGCATCGGCCATCAGCAAATGGTCGAGATTGCCCGCAACCTGATC  >  NC_002947/2801829‑2802120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑