Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,951,334 G→C 9.5% intergenic (‑113/+48) oguA ← / ← PP_2585 8‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,951,3340GC 9.5% 116.3 / 4.9 42intergenic (‑113/+48)oguA/PP_25858‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (22/16);  new base C (2/2);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.35e-01

GAGGAGCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAG  >  NC_002947/2951189‑2951478
                                                                                                                                                 |                                                                                                                                                
gaggagCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTcgcg                                                                                                                                               >  3:148292/1‑149 (MQ=255)
 aggagCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTggcgg                                                                                                                                              <  7:306469/149‑1 (MQ=255)
      cGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCtct                                                                                                                                          <  6:304392/148‑3 (MQ=255)
       gTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTagag                                                                                                                                        >  3:338196/1‑149 (MQ=255)
              cAGGGTTTGTAGATGACATGGTGCAAGCCTTCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCaa                                                                                                                                 <  4:148292/149‑1 (MQ=255)
              cAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGATCGAAGTGCCCGTTCCGGCCTCTTGGCGGCTAGAGCcggg                                                                                                                                   >  5:10380/1‑145 (MQ=255)
                             aCATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGt                                                                                                                  >  2:217690/1‑149 (MQ=255)
                              cATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAACCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGtt                                                                                                                 <  2:319296/149‑1 (MQ=255)
                                  gTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCa                                                                                                              <  4:338196/148‑1 (MQ=255)
                                   tGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGc                                                                                                            >  6:269422/1‑149 (MQ=255)
                                           tGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGa                                                                                                       >  3:342916/1‑146 (MQ=255)
                                                          ggCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTcgc                                                                                     <  5:111263/149‑1 (MQ=255)
                                                             gATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGgc                                                                                  >  1:243507/1‑149 (MQ=255)
                                                                   cGATGTTATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTca                                                                            >  3:68449/1‑149 (MQ=255)
                                                                   cGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTCATTGTATACAGCTCCAAGTCCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGCGGCCGGCACGGGTTCCAGCCATGAAAGCTCACACCGCCTCGCGGCGGCTca                                                                            >  8:88138/1‑149 (MQ=255)
                                                                            aGGAGCTCGGTATTGTAAGCAAGCTTTTTACTGATTTTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGATCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCt                                                                      <  6:10380/146‑1 (MQ=255)
                                                                                 cTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGCGGCCGGCCCGGGTTGCAGCCCTGAAAGATCACCCCGCCTCGCGGCTGCGCACACACGCCAGcccc                                                              >  8:310855/1‑146 (MQ=255)
                                                                                  tCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAAcc                                                             >  7:340024/1‑149 (MQ=255)
                                                                                     gTATTGTAATCAAGCTTTTTACTGATTGTATACCGCTCTAAGTGCCCGGGCCCGCCTCGTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCggg                                                          <  8:188412/149‑1 (MQ=255)
                                                                                      tATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCggg                                                          <  8:340024/148‑1 (MQ=255)
                                                                                           tAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATcc                                                    >  4:346422/1‑149 (MQ=255)
                                                                                              gcaagcTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCa                                                 <  4:68449/149‑1 (MQ=255)
                                                                                                    tttttACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGt                                           <  2:243507/149‑1 (MQ=255)
                                                                                                     ctttACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGt                                           >  1:150792/2‑148 (MQ=255)
                                                                                                           tGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGtt                                    >  5:59373/1‑149 (MQ=255)
                                                                                                           tGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGtt                                    >  6:173800/1‑149 (MQ=255)
                                                                                                            gATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACCCCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGttt                                   >  5:281149/1‑149 (MQ=255)
                                                                                                            gATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGttt                                   >  2:46084/1‑149 (MQ=255)
                                                                                                                         tCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCg                      >  8:76135/1‑149 (MQ=255)
                                                                                                                         gcGAAGGGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCg                      <  7:310855/148‑1 (MQ=255)
                                                                                                                           gAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCgg                     >  7:204678/1‑148 (MQ=255)
                                                                                                                           gAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCgg                     >  3:64419/1‑148 (MQ=255)
                                                                                                                           gAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCgg                     <  8:255094/148‑1 (MQ=255)
                                                                                                                            aaGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCgg                     >  7:47726/1‑147 (MQ=255)
                                                                                                                            aaGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGAGCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCTACGCATCCGgca                   <  4:342916/149‑1 (MQ=255)
                                                                                                                                      gCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGtt                                    <  7:156770/122‑1 (MQ=255)
                                                                                                                                      gCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGtt                                    >  8:156770/1‑122 (MQ=255)
                                                                                                                                         ggCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAgg      <  1:46084/149‑1 (MQ=255)
                                                                                                                                          gCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAggg     <  6:59373/149‑1 (MQ=255)
                                                                                                                                          gCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAggg     <  8:47726/149‑1 (MQ=255)
                                                                                                                                            ctctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAggg     >  5:145823/1‑147 (MQ=255)
                                                                                                                                             tctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAg  >  8:94769/1‑149 (MQ=255)
                                                                                                                                               ttGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCa        >  3:339119/1‑141 (MQ=255)
                                                                                                                                               ttGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCa        <  4:339119/141‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GAGGAGCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAG  >  NC_002947/2951189‑2951478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑