Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,973,809	A→G	7.5%	L22P (CTG→CCG)	PP_2600 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,973,809	0	A	G	7.5%	181.4 / 4.0	66	L22P (CTG→CCG)	PP_2600	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/33);  new base G (2/3);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

TCTTCCTCGATGCCGGCCCTGCGCGCCAGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTCCACATTCTTATCAGCAGCGGGTGGTCCTGCAAGGTCAGGTTGGT  >  NC_002947/2973662‑2973952                                                                                                                                                    |                                                                                                                                                   tCTTCCTCGATGCCGGCCCTGCGCGCCAGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTACTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAg                                                                                                                                                   >  1:310648/1‑149 (MQ=255)      cTCGATGCCGGCCCTGCGCGCCAGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCcg                                                                                                                                               <  4:311897/148‑1 (MQ=255)       tCGATGCCGGCCCTGCGCGCCAGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCcggc                                                                                                                                             >  4:130870/1‑149 (MQ=255)             ccGGCCCTGCGCGCCAGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGgcgc                                                                                                                                        <  1:337442/148‑1 (MQ=255)              cGGCCCTGCGCGCCAGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCGGGCTGgcggcggcc                                                                                                                                      <  4:321854/149‑4 (MQ=255)              cGGCCCTGCGCGCCAGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCGGGCTGgcggcgg                                                                                                                                        >  6:260214/1‑146 (MQ=255)              cGGCCCTGCGCGCCAGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCGGGCTGgcggcgg                                                                                                                                        <  5:260214/147‑2 (MQ=255)               ggCCCTGCGCGCCAGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGgcgc                                                                                                                                        >  1:191505/1‑146 (MQ=255)                 cccTGCTCGCCAGCGTGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCTGTTCTTTCTCCTCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGTCCGCCGCCAGCCCGGCGGCGCCAGaa                                                                                                                                   <  6:46178/149‑1 (MQ=255)                   cTGCGCGCCAGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCGGGCTGgcggcgg                                                                                                                                        >  4:307003/1‑141 (MQ=255)                   cTGCGCGCCAGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCGGGCTGgcggcgg                                                                                                                                        <  3:307003/142‑2 (MQ=255)                      cgcgcCAGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTAt                                                                                                                              <  8:337665/149‑1 (MQ=255)                      cgcgcCAGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTAt                                                                                                                              <  5:138445/149‑1 (MQ=255)                            aGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAg                                                                                                                        <  4:177534/149‑1 (MQ=255)                                  ttGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATc                                                                                                                  >  5:314281/1‑149 (MQ=255)                                   ccgttctcACCCCGGGCCAGCCGCCGGTCCATGCGGTGGCAACGCCAAACACCGGTTCTTTCTCCGCCACGATCCCCTTCTGCCCATGCCAGGCATCCGTGACGGCCGCCGCCAGCCCGGGGGCGCCATAAACTATGACCAGTACATc                                                                                                                  <  6:247579/141‑1 (MQ=255)                                       tGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACAACGCAt                                                                                                              >  8:185492/1‑148 (MQ=255)                                         gCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCg                                                                                                           >  1:40074/1‑149 (MQ=255)                                          cACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTGCTCCGCCACGAGACCCTTCTGCACATGCCAGGCAGCAGTGAAGGCCGCCGCCAGCCCGGCTGCGCCCTCACAGAGGACCAGTACATCTCAGTCgg                                                                                                          >  6:6443/1‑149 (MQ=255)                                           aCCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGt                                                                                                         >  4:180466/1‑149 (MQ=255)                                           aCCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTAAATCGCGATCgg                                                                                                          >  5:247579/1‑148 (MQ=255)                                            cccAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGtt                                                                                                        >  7:114236/1‑149 (MQ=255)                                             ccAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGttt                                                                                                       >  4:400729/1‑149 (MQ=255)                                                 gCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCgag                                                                                                   >  1:72510/1‑149 (MQ=255)                                                    cAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCga                                                                                                    <  3:254750/145‑1 (MQ=255)                                                    cAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCga                                                                                                    >  4:254750/1‑145 (MQ=255)                                                    cAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGagag                                                                                                 >  3:358719/1‑148 (MQ=255)                                                     aGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAgagga                                                                                               <  3:400729/149‑1 (MQ=255)                                                         gccgGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCg                                                                                            >  4:85234/1‑148 (MQ=255)                                                           cgGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGaa                                                                                         <  8:244643/149‑1 (MQ=255)                                                            ggACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAg                                                                                        <  7:185492/149‑1 (MQ=255)                                                               ccAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCa                                                                                     >  2:90125/1‑149 (MQ=255)                                                                   gCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGTCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGAcc                                                                                                                          >  7:206170/1‑108 (MQ=255)                                                                   gCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGTCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGAcc                                                                                                                          <  8:206170/108‑1 (MQ=255)                                                                   gCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGc                                                                                 <  8:114236/149‑1 (MQ=255)                                                                    cGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCg                                                                                <  6:314281/149‑1 (MQ=255)                                                                         gCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑ttt                                                                        <  2:72510/149‑1 (MQ=255)                                                                          cACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTc                                                                       <  3:180466/149‑1 (MQ=255)                                                                                  aaCACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCtt                                                               <  5:134559/149‑1 (MQ=255)                                                                                    cacCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCtttt                                                             <  5:288913/149‑1 (MQ=255)                                                                                     acCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTtta                                                            <  3:130870/149‑1 (MQ=255)                                                                                     acCGGTTCTTTCTCCGCCACGATCACCTACTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTtta                                                            <  2:310648/149‑1 (MQ=255)                                                                                       cGGTTCTTTCTCCGCCACGATCCCCTTCTGCACATGCCCGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACAGCGCAGGCGGTTGGGAGAGGAGCGGACGGCATTGCGGG‑‑‑TTTCATGTGCTTttatt                                                          >  7:256712/1‑149 (MQ=255)                                                                                       cGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTttat                                                           >  2:285895/1‑148 (MQ=255)                                                                                        ggTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAATACATCGCATTCGGTTTCGAGAGGAGCGGAATGCATAGCGGG‑‑‑TTTCCTGTGCTTttatt                                                          >  4:288421/1‑148 (MQ=255)                                                                                         gTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTttat                                                           >  1:385528/1‑146 (MQ=255)                                                                                                   cGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATg                                                 <  4:358719/146‑1 (MQ=255)                                                                                                    gCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGATAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTcc                                             <  7:139840/149‑1 (MQ=255)                                                                                                    gCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTc                                              >  8:139840/1‑148 (MQ=255)                                                                                                         gATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTCCACAtt                                        <  1:285895/149‑1 (MQ=255)                                                                                                          aTCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTCCACATTc                                       <  3:85234/149‑1 (MQ=255)                                                                                                              ccTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTCCACATTCTTAt                                   >  5:354062/1‑149 (MQ=255)                                                                                                               cTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTCCACATTCTTAt                                   >  2:330562/1‑148 (MQ=255)                                                                                                               cTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATATTCCACATTCTTAt                                   >  1:269259/1‑148 (MQ=255)                                                                                                                       cATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTCCACATTCTTATCAGCAGCgg                          >  5:325654/1‑149 (MQ=255)                                                                                                                        aTGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTCCACATTCTTATCAGCAGCggg                         <  1:90125/149‑1 (MQ=255)                                                                                                                           ccAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTCCACATTCTTATCAGCAGCGggtgg                      <  2:40074/149‑1 (MQ=255)                                                                                                                             aGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTCCACATTCTTATCAGCAGCGGGTGGTc                    >  2:211726/1‑149 (MQ=255)                                                                                                                               gCAGCCGTGACTGCCGCCGCCAGCCCGGAGGCGCCAGAACCTTTGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTCTTAGAACAATGTTCCACATTCTTATCAGCAGCGggtgtgcct                  <  5:6443/149‑6 (MQ=255)                                                                                                                                    cGTGACGGCCGCCTCCAGCCCGTCTGCGCCAGTACCTATTAACAGTTCATCTCATTCGGTTTCGAGAGGATCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTCCACATTCTTATCAGCAGCGGGTGGTCCTGCAAg             <  3:288421/149‑1 (MQ=255)                                                                                                                                     gTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTCCACATTCTTATCAGCAGCGGGTGGTCCt                  >  2:351783/1‑143 (MQ=255)                                                                                                                                     gTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTCCACATTCTTATCAGCAGCGGGTGGTCCt                  <  1:351783/143‑1 (MQ=255)                                                                                                                                         cGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTCCACATTCTTATCAGCAGCGGGTGGTCCTGCAAGGTCAg        <  4:41638/149‑1 (MQ=255)                                                                                                                                             cgccgccACGCCGGCGGCGCCATTACCTATGTCCATGACATGGGATTCGGTTTGGTGATGAGGGGAAGGGATTTGGGG‑‑‑GTTCCTGTTGTTTTATTAGTACAATGTTCCACATTCTTATCAGCAGCGGGGGGTCCTGCAAGGTCAGGTTg    <  6:354062/149‑1 (MQ=255)                                                                                                                                               ccgccAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATATTCCACATTCTTATCAGCAGCGGGTGGTCCTGCAAGGTCAGGTTGGt  <  2:269259/149‑1 (MQ=255)                                                                                                                                                ggccAGCCGGGGGGCGCAATAACCTTTTACATTTACATGGAATTGGGTTTGTTGTGTAGCGGTATGCATTGGGGCTCCTTTCCTGTGCTTTTATTAGAACAATGTTCCACATTCTTATCAGCAGCGGGTGGTCCTGCAAGGTCAGGTTg    <  1:330562/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TCTTCCTCGATGCCGGCCCTGCGCGCCAGCGGGTTGCGTGGCACCCAGGCCCAGCCGCCGGACCAGGCGGTGGCACCGCCAAACACCGGTTCTTTCTCCGCCACGATCACCTTCTGCCCATGCCAGGCAGCCGTGACGGCCGCCGCCAGCCCGGCGGCGCCAGAACCTATGACCAGTACATCGCATTCGGTTTCGAGAGGAGCGGAAGGCATTGCGGG‑‑‑TTTCCTGTGCTTTTATTAGAACAATGTTCCACATTCTTATCAGCAGCGGGTGGTCCTGCAAGGTCAGGTTGGT  >  NC_002947/2973662‑2973952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑