Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	233,834	A→T	13.1%	intergenic (+22/+41)	PP_0180 → / ← PP_0181	cytochrome c family protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	233,834	0	A	T	13.1%	148.4 / 14.1	68	intergenic (+22/+41)	PP_0180/PP_0181	cytochrome c family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/33);  new base T (5/4);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.47e-01

TTCGACTGGCTGGGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGT  >  NC_002947/233687‑233976                                                                                                                                                    |                                                                                                                                                ttCGACTGGCTGGGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTTGCTGGCAGAAAAGCGCAGTGCTGCGGCCAACTGCCTGGAGCCTGCGCGGGCCCGCAg                                                                                                                                                >  8:277008/1‑149 (MQ=255) ttCGACTGGCTGGGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAg                                                                                                                                                >  5:132321/1‑149 (MQ=255)   cGACTGGCGGGGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCGCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAgg                                                                                                                                               <  4:316618/148‑1 (MQ=255)      ctggctggGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGAcc                                                                                                                                           <  5:81297/149‑1 (MQ=255)      ctggctggGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGAcc                                                                                                                                           <  6:132321/149‑1 (MQ=255)         gctggGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACcgc                                                                                                                                         <  7:135349/148‑1 (MQ=255)         gctggGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACcg                                                                                                                                          <  8:134768/147‑1 (MQ=255)           tggGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCgcgc                                                                                                                                       >  1:228624/1‑148 (MQ=255)             gggATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCTGTGGGACCGCGCa                                                                                                                                      <  4:303944/147‑1 (MQ=255)             cggctacATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCaa                                                                                                                                     <  5:49769/142‑1 (MQ=255)               gATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGt                                                                                                                                 <  2:234072/149‑1 (MQ=255)                aTTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGtc                                                                                                                                <  4:309666/149‑1 (MQ=255)                aTTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGt                                                                                                                                 >  8:32780/1‑148 (MQ=255)                 ttCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGtct                                                                                                                               >  8:369783/1‑149 (MQ=255)                   cATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGGAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGtct                                                                                                                               >  1:300726/1‑147 (MQ=255)                           tGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTgc                                                                                                                     <  1:70226/149‑1 (MQ=255)                                  tCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGTCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGc                                                                                                               >  2:26332/1‑148 (MQ=255)                                  tCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGGCTCAGATCTTGCGCAAGcc                                                                                                              >  8:247125/1‑149 (MQ=255)                                    gCTGTCGGCACAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTg                                                                                                            <  1:196701/149‑1 (MQ=255)                                          ggCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑g                                                                                                                                   <  6:112579/120‑1 (MQ=255)                                          ggCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑g                                                                                                                                   >  5:112579/1‑120 (MQ=255)                                          ggCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGtctc                                                                                                                              <  1:17782/125‑1 (MQ=255)                                          ggCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGtctc                                                                                                                              >  2:17782/1‑125 (MQ=255)                                          ggCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTc                                                                                                      <  7:363964/149‑1 (MQ=255)                                          ggCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTc                                                                                                      <  7:358633/149‑1 (MQ=255)                                           gcgcAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGCTGCCCCCGGGACCCCCCCA‑CGGCTCAAAGAGTGAGCAAGCCGGAAGCTc                                                                                                      >  4:5726/1‑148 (MQ=255)                                                 gCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGGAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGAGCATCAggcttg                                                                                               <  2:300726/149‑3 (MQ=255)                                                      ggCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCCAGCCTGAGCATCAggcttccgcaa                                                                                          <  7:369783/149‑8 (MQ=255)                                                       gCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTgg                                                                                         >  1:90939/1‑149 (MQ=255)                                                        ccTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTg                                                                                                                      <  3:210783/119‑1 (MQ=255)                                                        ccTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCaa                                                                                                                 >  1:340465/1‑124 (MQ=255)                                                        ccTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCaa                                                                                                                 <  2:340465/124‑1 (MQ=255)                                                            gCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTTGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGgcgc                                                                                      <  6:288983/147‑1 (MQ=255)                                                                gCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGTCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTga                                                                                 <  1:26332/148‑1 (MQ=255)                                                                gCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTga                                                                                 <  7:247125/148‑1 (MQ=255)                                                                gCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTg                                                                                  <  2:90939/147‑1 (MQ=255)                                                                gCCATCGTGTTCCTTTACGGCCGCGCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCTGTGGGACCGCGCa                                                                                                                                      <  3:69259/96‑1 (MQ=39)                                                                  cATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCCCAGGGCCCGCGCCA‑GGTCTCAGATCTTTCGCAAGCCGGATGCGCAGGCTGGCAGTGGCGCCGTGAGaa                                                                              >  7:168599/1‑149 (MQ=255)                                                                   aTCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAAt                                                                             <  2:228624/149‑1 (MQ=255)                                                                   aTCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCCCCGTCCCACAGGGACCCCGCAA‑GGTCACAGATCATGCGCAAGCCAGAAGCTCAAGCTGGCAGTGGCGCCATGAGTAt                                                                             >  6:135715/1‑149 (MQ=255)                                                                        gtTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCCGTGCCACAGGGACCGCGCAA‑GGTGTCAGATCATGCGCAAGCCAGATGCTCAGGCTGGCTGTGGGGGCGTGAGaaatttat                                                                        >  1:327265/1‑143 (MQ=255)                                                                           ccTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACACGGTCCCCGCAA‑GGTCTCAGATATTTCGCAACCCAGATGCTAAGGCTGGCTGTGGCGCCATGAGAATGTTCTg                                                                       >  8:20202/1‑147 (MQ=255)                                                                           ccTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGGCCCCCACGGGCCCCGCCA‑GGTGGCAGATATTTGGGAAACCAGATGGTAAGGGGGGCGGTGGGGGCGGGAGAATGTTGTGTa                                                                     >  4:315025/1‑149 (MQ=255)                                                                               tACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGc                                                                   <  7:32780/147‑1 (MQ=255)                                                                                 agggcGCTAGTGGCTGGCCGAAAAGGGGATTGCTGCGGCCAACTGTCTGGAGCCTGCGCGGTCCCACAGGGAACGCGCAA‑TGTGTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAAt                                                                <  7:277008/144‑1 (MQ=255)                                                                                           tggctggcCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTc                                                     <  7:20202/149‑1 (MQ=255)                                                                                             gctggcCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCaa                                                   <  3:315025/149‑1 (MQ=255)                                                                                                 gcCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTgg                                                                                         <  7:155681/107‑1 (MQ=255)                                                                                                    gAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTc                                            <  1:4481/149‑1 (MQ=255)                                                                                                    gAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTc                                            <  5:135715/149‑1 (MQ=255)                                                                                                                 ctgcGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCt                                <  3:5726/148‑1 (MQ=255)                                                                                                                     ggCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCa                           <  8:168599/149‑1 (MQ=255)                                                                                                                           ctgactgGAGCCTGCGCGGGCCCACACGGACCGCCCAA‑AGTCGCAGATCTTGCGCAAGCCGGATGGTCACGCTGGCTGTGGCGCCGTGAGAAAGTTCTGTTTCAATTTGACACATTAAACGGCGTCATGACCCACGCGGGTCAGGTAga                     >  6:105759/1‑148 (MQ=255)                                                                                                                              actgGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAgccg                   <  5:105759/148‑1 (MQ=255)                                                                                                                                     ccTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCgg           >  6:258248/1‑149 (MQ=255)                                                                                                                                      ttGCGCGGTCCCTGTGGGACCGCGCAAGGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCgg           >  1:24771/2‑149 (MQ=255)                                                                                                                                      ttGCGCGGTCCCTGTGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGc          >  7:196100/2‑149 (MQ=255)                                                                                                                                         cgcgGTCCCTGTGGGACCGCGCAG‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTc                            >  4:44941/1‑128 (MQ=255)                                                                                                                                         cgcgGTCCCTGTGGGACCGCGCAG‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTc                            <  3:44941/128‑1 (MQ=255)                                                                                                                                          gcgGTCCCTGTGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGAt      >  6:266355/1‑149 (MQ=255)                                                                                                                                          gcgGTCCCTGTGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGACTGAt      >  2:230358/1‑149 (MQ=255)                                                                                                                                           cgGTCCCTGTGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATc     <  8:196100/149‑1 (MQ=255)                                                                                                                                            ggTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCg    >  1:163953/1‑149 (MQ=255)                                                                                                                                              tCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCATGCTGGCTGTGGCGCCTTGAGAATGTACTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGTTCAGGTAGACGCCATCCGGCTGaactgt  >  8:262673/1‑144 (MQ=255)                                                                                                                                              tCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGt  >  8:262670/1‑149 (MQ=255)                                                                                                                                                ccACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGt  >  3:270721/1‑147 (MQ=255)                                                                                                                                                ccACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGt  >  3:107584/1‑147 (MQ=255)                                                                                                                                                ccACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGt  >  4:24745/1‑147 (MQ=255)                                                                                                                                                    |                                                                                                                                                TTCGACTGGCTGGGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAA‑GGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGT  >  NC_002947/233687‑233976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑