Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,019,936 T→A 8.6% N158K (AAT→AAA)  PP_2638 → cellulose synthase operon protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,019,9360TA 8.6% 195.9 / 7.3 70N158K (AAT→AAA) PP_2638cellulose synthase operon protein C
Reads supporting (aligned to +/- strand):  ref base T (30/34);  new base A (3/3);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.20e-01

AACCGGCGCCTGCACGGCGCTTGCAGCAGGCGCGTCTGCTGGCAGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGCCTTGGCCCTGCTCGATCAGCTG  >  NC_002947/3019788‑3020083
                                                                                                                                                    |                                                                                                                                                   
aacCGGCGCCTGCACGGCGCTTGCAGCAGGCGCGTCTGCTGGCAGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAAt                                                                                                                                                     <  1:174307/149‑1 (MQ=255)
            cACGGCGCGTGCAGCAGGCGCGTCTGCTGGCAGTTACCGGCCGCAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCa                                                                                                                                          <  6:131139/148‑1 (MQ=255)
                    tgccagcagACGCGCCTGCTGGCAGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCt                                                                                                                                   >  5:240503/4‑147 (MQ=255)
                      gcagcaGGCGCGTCTGCTGGCAGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAg                                                                                                                                <  6:416188/148‑1 (MQ=255)
                      gcagcaGGCGCGTCTGCTGGCAGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAg                                                                                                                                <  3:63468/148‑1 (MQ=255)
                       cagcagGCGCGTCTGCTGGCAGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGc                                                                                                                              >  1:182784/1‑149 (MQ=255)
                       cagcagGCGCGTCTGCTGGCAGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGc                                                                                                                              >  6:228429/1‑149 (MQ=255)
                        agcagGCGCGTCTGCTGGCAGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCAAACGCCCTGAGGc                                                                                                                              >  2:74673/1‑148 (MQ=255)
                           agGCGCGTCTGCTGGCAGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATc                                                                                                                          <  5:166894/149‑1 (MQ=255)
                                cgTCTGCTGGCAGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCa                                                                                                                     <  5:277409/149‑1 (MQ=255)
                                      ctgGCAGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGCCCAACGCCCTGAGGCGATCCGCCAGTTg                                                                                                                 >  2:12577/1‑147 (MQ=255)
                                         gCAGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCg                                                                                                            <  7:180062/149‑1 (MQ=255)
                                           aGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGt                                                                                                          <  5:3376/149‑1 (MQ=255)
                                           aGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGt                                                                                                          >  1:223202/1‑149 (MQ=255)
                                            gTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGtt                                                                                                         <  6:240503/149‑1 (MQ=255)
                                            gTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGtt                                                                                                         <  3:132798/149‑1 (MQ=255)
                                                ccGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGAt                                                                                                     <  8:382313/149‑1 (MQ=255)
                                                 cGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGCCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATc                                                                                                    <  1:12577/149‑1 (MQ=255)
                                                  ggCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGAt                                                                                                     >  4:217681/1‑147 (MQ=255)
                                                  ggCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGAt                                                                                                     >  8:40897/1‑147 (MQ=255)
                                                  ggCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAAACTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGAt                                                                                                     >  1:46791/1‑147 (MQ=255)
                                                  ggCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAAACTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGAt                                                                                                     >  1:40802/1‑147 (MQ=255)
                                                  ggCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAAACTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGAt                                                                                                     >  1:258842/1‑147 (MQ=255)
                                                     cGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTTCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATcagca                                                                                                <  8:285113/149‑1 (MQ=255)
                                                           gAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTAccc                                                                                          >  1:239063/1‑149 (MQ=255)
                                                              gagGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCggg                                                                                       >  4:255966/1‑149 (MQ=255)
                                                                ggCGCTGGCGGTATACGTACAGTTGTTTGCCGGCGAACCACCGAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGa                                                                                                                                 <  8:210496/105‑1 (MQ=255)
                                                                 gcgcTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAg                                                                                    >  2:374550/1‑149 (MQ=255)
                                                                       gCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCgg                                                                              <  2:182784/149‑1 (MQ=255)
                                                                        cGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCggg                                                                             <  5:351214/149‑1 (MQ=255)
                                                                         ggTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGc                                                                            >  6:254060/1‑149 (MQ=255)
                                                                             ctcgtaaaGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCGTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACGCGGGCAGCGCCGGGCTgc                                                                         <  2:337612/141‑1 (MQ=255)
                                                                               cGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTgcgc                                                                       <  2:223202/148‑1 (MQ=255)
                                                                               cGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCAAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTgcgc                                                                       >  5:260754/1‑148 (MQ=255)
                                                                                gAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGATGCAACGGATGGATCAGCAGTACCCGGGCAGCGCCGGGCTgc                                                                         >  1:337612/1‑145 (MQ=255)
                                                                                 aaCAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTgcgc                                                                       >  5:107726/1‑146 (MQ=255)
                                                                                     gttgttTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGCGGCGAGCCGCCAGTTGCAGCGGTTGGAACAGCGGTACCCGGGACGCGCCGGGCTCCGCCAGcccc                                                                >  4:232219/1‑149 (MQ=255)
                                                                                     gttgttTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGAccc                                                                <  5:130411/149‑1 (MQ=255)
                                                                                      ttgttTGCTGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCct                                                               >  4:247771/1‑149 (MQ=255)
                                                                                         tttGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCctggc                                                            <  5:228429/149‑1 (MQ=255)
                                                                                         tttGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAAACTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCctggc                                                            <  2:258842/149‑1 (MQ=255)
                                                                                         tttGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAAACTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCctggc                                                            <  2:40802/149‑1 (MQ=255)
                                                                                         tttGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAAACTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCctggc                                                            <  2:46791/149‑1 (MQ=255)
                                                                                             ccGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCctggctgg                                                         <  5:254060/148‑1 (MQ=255)
                                                                                              cGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGAGCCGGGCTGCGCCAGACCCTGGCtggtt                                                       >  7:26440/1‑149 (MQ=255)
                                                                                               ggCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCtggttg                                                      >  7:164002/1‑149 (MQ=255)
                                                                                                gCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCtggttgg                                                     <  1:374550/149‑1 (MQ=255)
                                                                                                 cGAACCACCCCGCCGTGAATTGGCCCTGGTCGTCCGGCGGGGGCGGGGGAATCTGGCATGGCAACGGCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCtggttggt                                                    <  3:232219/149‑1 (MQ=255)
                                                                                                     ccaccCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGgttgtt                                                <  2:239063/149‑1 (MQ=255)
                                                                                                      caccCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTgcgc                                                                       >  4:196647/1‑125 (MQ=255)
                                                                                                      caccCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTgcgc                                                                       <  3:196647/125‑1 (MQ=255)
                                                                                                       accCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGACCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCg                                              >  8:90283/1‑149 (MQ=255)
                                                                                                         ccAGCCTTGAATTGGCCCCGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGcc                                            >  5:256893/1‑149 (MQ=255)
                                                                                                                     tGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGct                                                                                                                                                >  1:66070/1‑36 (MQ=255)
                                                                                                                     tGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGc                                                                                                                                                 <  2:66070/36‑1 (MQ=255)
                                                                                                                         ccTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACcgc                             >  2:193051/1‑148 (MQ=255)
                                                                                                                             gACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGc                        <  6:107726/149‑1 (MQ=255)
                                                                                                                             gACTACTGGCGCGTGCGCGGCAATCTGCCAGGCAAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAg                          <  1:74673/147‑1 (MQ=255)
                                                                                                                                tactGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGCCtt                     >  6:105065/1‑149 (MQ=255)
                                                                                                                                  ctGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGCCtt                     <  3:255966/147‑1 (MQ=255)
                                                                                                                                  ctGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGCCTTgg                   >  3:236748/1‑149 (MQ=255)
                                                                                                                                  ctGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGCCTTgg                   <  3:217681/149‑1 (MQ=255)
                                                                                                                                  ctGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGCCTTg                    <  7:40897/148‑1 (MQ=255)
                                                                                                                                  ctGGCGCGTGCGCGGCAATCTGCCAGGCCAACCCCCTGAGGCGATCCGCCAGATGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGATGTTCGCCGAAAAGCGTGACCGCGAGGCCTTgg                   >  2:87295/1‑149 (MQ=255)
                                                                                                                                     gcgcGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGCCTTGGccc                >  6:410051/1‑149 (MQ=255)
                                                                                                                                         gTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGCCTTGGCCCTGCt            <  8:164002/149‑1 (MQ=255)
                                                                                                                                          tGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGCCTTGGCCCTGCTc           <  4:236748/149‑1 (MQ=255)
                                                                                                                                            cgcgGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGCCTTGGCCCTGCTCg          <  8:26440/148‑1 (MQ=255)
                                                                                                                                                  aaTCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGCCTTGGCCCTGCTCGATCAGCt   >  7:47473/1‑149 (MQ=255)
                                                                                                                                                  aaTCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGCCTTGGCCCTGCTCGATCAGCt   >  4:352930/1‑149 (MQ=255)
                                                                                                                                                   aTCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGCCTTGGCCCTGCTCGATCAGCTg  <  2:116083/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AACCGGCGCCTGCACGGCGCTTGCAGCAGGCGCGTCTGCTGGCAGTTACCGGCCGTAATGAAGAGGCGCTGGCGGTATACGAACAGTTGTTTGCCGGCGAACCACCCAGCCTTGAATTGGCCCTGGACTACTGGCGCGTGCGCGGCAATCTGCCAGGCCAACGCCCTGAGGCGATCCGCCAGTTGCAGCGGTTGGATCAGCAGTACCCGGGCAGCGCCGGGCTGCGCCAGACCCTGGCTGGTTGGTTGTTCGCCGAAAAGCGTGACCGCGAGGCCTTGGCCCTGCTCGATCAGCTG  >  NC_002947/3019788‑3020083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑