Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,023,034 G→A 7.7% intergenic (+53/‑285) PP_2638 → / → dapA‑II cellulose synthase operon protein C/4‑hydroxy‑tetrahydrodipicolinate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,023,0340GA 7.7% 146.7 / 2.1 52intergenic (+53/‑285)PP_2638/dapA‑IIcellulose synthase operon protein C/4‑hydroxy‑tetrahydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  ref base G (23/25);  new base A (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01

ATGGCATGCTCTACCTGCGCTACCTGTTCGAACCGTGGCGAGGC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCCAGGCAGGCGCGTGCGTTGCCGTTGCCAAGAAATCCAAGA  >  NC_002947/3022887‑3023182
                                                                                                                                                    |                                                                                                                                                    
aTGGCATGCTCTACCTGCGCTACCTGTTCGAACCGTGGCGAGGC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAgg                                                                                                                                                     >  6:156520/1‑149 (MQ=255)
   gCATGCTCTACCTGCGCTACCTGTTCGAACCGTGGCGAGGC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGt                                                                                                                                                   <  2:392056/148‑1 (MQ=255)
           tACCTGCGCTACCTGTTCGAACCGTGGCGAGGCAAACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGacac                                                                                                                                             >  1:266527/1‑147 (MQ=255)
             ccTGCGCTACCTGTTCGAACCGTGGCGAGGC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAgg                                                                                                                                         <  3:99985/148‑1 (MQ=255)
                      ccTGTTCGAACCGTGGCGAGGC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCAcgccgc                                                                                                                                 <  8:125507/147‑1 (MQ=255)
                        tGTTCGAACCGTGGCGAGGC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGt                                                                                                                                                   <  8:214127/127‑1 (MQ=255)
                        tGTTCGAACCGTGGCGAGGC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACAACAGGCACGCCGCCcca                                                                                                                             >  2:146590/1‑149 (MQ=255)
                          ttCGAACCGTGGCGAGGC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCccac                                                                                                                            >  8:235899/1‑148 (MQ=255)
                          ttCGAACCGTGGCGAGGC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCccac                                                                                                                            <  8:227430/148‑1 (MQ=255)
                          ttCGAACCGTGGCGAGGC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCccac                                                                                                                            >  2:395571/1‑148 (MQ=255)
                                ccGTGGCGAGGC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATc                                                                                                                     >  2:126019/1‑149 (MQ=255)
                                ccGTGGCGAGGC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATc                                                                                                                     <  8:114957/149‑1 (MQ=255)
                                ccGGGGCGAGGC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATc                                                                                                                     <  1:29638/149‑1 (MQ=255)
                                  gTGGCGACGC‑AACCTAGCCCTGCCGGGGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGGTGACACCAGTCACGCCGCCCCACCATTATCCa                                                                                                                   <  3:221256/149‑1 (MQ=255)
                                         ggC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCATCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATAc                                                                                                            <  3:178261/149‑1 (MQ=255)
                                          gC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATAcc                                                                                                           >  3:156919/1‑149 (MQ=255)
                                           c‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCt                                                                                                          <  7:235899/149‑1 (MQ=255)
                                              agcTAGCCCTGCCGGTGACACCGCTGGAGCCTTCGAGCGAGTGGCGTTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCGACCATTATCCAGCCATACCTAc                                                                                                        <  2:266527/147‑1 (MQ=255)
                                              aCCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCa                                                                                                                   >  2:197934/1‑138 (MQ=255)
                                              aCCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCa                                                                                                                   <  1:197934/138‑1 (MQ=255)
                                              aCCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTAc                                                                                                        >  4:341274/1‑149 (MQ=255)
                                                     ccTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACgg                                                                                                 <  7:197500/149‑1 (MQ=255)
                                                         ccGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCtt                                                                                             <  1:395571/149‑1 (MQ=255)
                                                               acacCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCaa                                                                                        <  2:334098/148‑1 (MQ=255)
                                                                 acCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACAt                                                                                     >  5:168290/1‑149 (MQ=255)
                                                                  tcGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCTCACCATTATCCa                                                                                                                   >  5:295990/2‑118 (MQ=255)
                                                                  tcGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCTCACCATTATCCa                                                                                                                   <  6:295990/117‑1 (MQ=255)
                                                                    gCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTc                                                                                  <  3:341274/149‑1 (MQ=255)
                                                                        gAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAAc                                                                                       <  8:68335/140‑1 (MQ=255)
                                                                        gAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAAc                                                                                       >  7:68335/1‑140 (MQ=255)
                                                                         aGCCGTCCAGCGTGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGGGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCAGCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACAt                                                                             <  6:54570/149‑1 (MQ=255)
                                                                           ccTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAAGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATc                                                                            >  3:297331/1‑148 (MQ=255)
                                                                           ccTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTACAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAAGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATc                                                                            >  1:70906/1‑148 (MQ=255)
                                                                                 aGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCAc                                                                     >  3:318409/1‑149 (MQ=255)
                                                                                     aGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCa                                                                 >  8:284335/1‑149 (MQ=255)
                                                                                      gTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCAAt                                                                >  8:325452/1‑149 (MQ=255)
                                                                                       tGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCAt                                                                <  5:33854/148‑1 (MQ=255)
                                                                                         gCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTc                                                             >  6:401172/1‑149 (MQ=255)
                                                                                                 ggCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCATCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCgg                                                     >  2:80332/1‑149 (MQ=255)
                                                                                                            cGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGcc                                          >  1:405349/1‑149 (MQ=255)
                                                                                                                 cAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCcagg                                      <  6:168290/148‑1 (MQ=255)
                                                                                                                 cAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCcagg                                      >  6:11909/1‑148 (MQ=255)
                                                                                                                 cAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAAGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCcagg                                      <  2:70906/148‑1 (MQ=255)
                                                                                                                 cAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAAGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCcagg                                      <  4:297331/148‑1 (MQ=255)
                                                                                                                  aaGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACC‑ACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCcaggcag                                   <  3:149148/149‑1 (MQ=255)
                                                                                                                          cgcCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCCAGGCAGGCgcctg                            >  8:321345/1‑149 (MQ=255)
                                                                                                                             cTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTTTCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCAGCTTCGGGCTTCATCGCCCAGGCAGGcgcgcccct                         >  6:76886/1‑144 (MQ=255)
                                                                                                                                cACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCCAGGCAGGCGCGTGcgtt                        <  4:318409/147‑1 (MQ=255)
                                                                                                                                   ggggCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCCAGGCAGGCGCGTGcgttgccgt                   >  4:362602/1‑149 (MQ=255)
                                                                                                                                    gggCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCCAGGTAGGCGCGTGcgttgccgtt                  <  1:146590/149‑1 (MQ=255)
                                                                                                                                     ggCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCCAGGCAGGCGCGTGcgttgccgttg                 <  5:401172/149‑1 (MQ=255)
                                                                                                                                             gggCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCCAGGCAGGCGCGTGCGTTGCCGTTGCCAAGaaa         >  6:217931/1‑149 (MQ=255)
                                                                                                                                                ccAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCCAGGCAGGCGCGTGCGTTGCCGTTGCCAAGAAATc       >  7:335991/1‑148 (MQ=255)
                                                                                                                                                    gggTTGACACCAGGCGCGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCCAGGCAGGCGCGTGCGTTGCCGTTGCCAAGAAATCCAAg   >  7:73733/1‑148 (MQ=255)
                                                                                                                                                    gggTTGACACCAGGCACGCCGCCCCATCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCCAGGCAGGCGCGTGCGTTGCCGTTGCCAAGAAATCCAAGa  <  1:80332/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATGGCATGCTCTACCTGCGCTACCTGTTCGAACCGTGGCGAGGC‑AACCTAGCCCTGCCGGTGACACCGCTGGAGCCTTCCAGCGAGTGGCGGTGACGGCAATCGCTGCGTTGCAAGGGGCGCGCCTGCACGGGGCGATGCGGGCCAAGGGTTGACACCAGGCACGCCGCCCCACCATTATCCAGCCATACCTACGCAACGGGCTTGCCAACATGTCCACATCCAACCACGCCATTTCCGCTTCGGGCTTCATCGCCCAGGCAGGCGCGTGCGTTGCCGTTGCCAAGAAATCCAAGA  >  NC_002947/3022887‑3023182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑